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Here we show that during prophase the centrosomes-nucleus axis reorients, so that centrosomes are positioned on the shortest nuclear axis at nuclear envelope (NE) breakdown. This centrosomes-nucleus configuration depends on mechanical cues generated by mitotic chromosome condensation on the prophase nucleus. We further show these mechanosensitive cues act through SUN1\/2 and NudE+NudEL to enable the polarized loading of Dynein on the NE. Finally, we observe this centrosome configuration favors the establishment of an initial bipolar spindle scaffold, facilitating chromosome capture and accurate segregation, without compromising division plane orientation. We propose that chromosome segregation fidelity depends on the mechanical properties of the prophase nucleus that facilitate spindle assembly by regulating NE-Dynein localization.<\/jats:p>","DOI":"10.1101\/526939","type":"posted-content","created":{"date-parts":[[2019,1,22]],"date-time":"2019-01-22T08:37:23Z","timestamp":1548146243000},"source":"Crossref","is-referenced-by-count":4,"title":["Mechanosensitive nuclear asymmetries define a bipolar spindle scaffold to ensure mitotic fidelity"],"prefix":"10.64898","author":[{"given":"Vanessa","family":"Nunes","sequence":"first","affiliation":[]},{"given":"Margarida","family":"Dantas","sequence":"additional","affiliation":[]},{"given":"Domingos","family":"Castro","sequence":"additional","affiliation":[]},{"given":"Elisa","family":"Vitiello","sequence":"additional","affiliation":[]},{"given":"Ir\u00e8ne","family":"Wang","sequence":"additional","affiliation":[]},{"given":"Nicolas","family":"Carpi","sequence":"additional","affiliation":[]},{"given":"Martial","family":"Balland","sequence":"additional","affiliation":[]},{"given":"Matthieu","family":"Piel","sequence":"additional","affiliation":[]},{"given":"Paulo","family":"Aguiar","sequence":"additional","affiliation":[]},{"given":"Helder","family":"Maiato","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6802-3696","authenticated-orcid":false,"given":"Jorge G.","family":"Ferreira","sequence":"additional","affiliation":[]}],"member":"54368","reference":[{"key":"2019112004550463000_526939v2.1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm.2016.162"},{"key":"2019112004550463000_526939v2.2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0169(1996)35:4<298::AID-CM3>3.0.CO;2-3"},{"key":"2019112004550463000_526939v2.3","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.150.5.975"},{"key":"2019112004550463000_526939v2.4","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2016.01.077"},{"key":"2019112004550463000_526939v2.5","doi-asserted-by":"crossref","unstructured":"Boudreau, V. , Chen, R. , Edwards, A. , Sulaimain, M. , and Maddox, P.S . 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