{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T11:52:39Z","timestamp":1768477959437,"version":"3.49.0"},"posted":{"date-parts":[[2019,3,21]]},"group-title":"Developmental Biology","reference-count":70,"publisher":"openRxiv","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2020,11,20]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                <jats:p>\n                  The vertebrate body is built during embryonic development by the sequential addition of new tissue as the embryo grows at its caudal end. During this process, the neuro-mesodermal progenitors (NMPs) generate the postcranial neural tube and paraxial mesoderm. Recently, several approaches have been designed to determine their molecular fingerprint but a simple method to isolate NMPs from embryos without the need for transgenic markers is still missing. We isolated NMPs using a genetic strategy that exploits their self-renew properties, and searched their transcriptome for cell surface markers. We found a distinct Epha1 expression profile in progenitor-containing areas of the mouse embryo, consisting of two cell subpopulations with different Epha1 expression levels. We show that Sox2\n                  <jats:sup>+<\/jats:sup>\n                  \/T\n                  <jats:sup>+<\/jats:sup>\n                  cells are preferentially associated with the Epha1 compartment, indicating that NMPs might be contained within this cell pool. Transcriptional profiling showed enrichment of high Epha1-expressing cells in known NMP and early mesoderm markers. Also, tail bud cells with lower Epha1 levels contained a molecular signature suggesting the presence of notochord progenitors. Our results thus indicate that Epha1 could represent a valuable cell surface marker for different subsets of axial progenitors, most particularly for NMPs taking mesodermal fates.\n                <\/jats:p>","DOI":"10.1101\/584524","type":"posted-content","created":{"date-parts":[[2019,3,21]],"date-time":"2019-03-21T22:19:17Z","timestamp":1553206757000},"source":"Crossref","is-referenced-by-count":4,"title":["Epha1 is a cell surface marker for neuromesodermal progenitors and their early mesoderm derivatives"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4666-5388","authenticated-orcid":false,"given":"Luisa","family":"de Lemos","sequence":"first","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3337-6373","authenticated-orcid":false,"given":"Andr\u00e9","family":"Dias","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5668-5630","authenticated-orcid":false,"given":"Ana","family":"N\u00f3voa","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9744-0912","authenticated-orcid":false,"given":"Mois\u00e9s","family":"Mallo","sequence":"additional","affiliation":[]}],"member":"54368","reference":[{"key":"2020112307500999000_584524v2.1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.303504"},{"key":"2020112307500999000_584524v2.2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.855001"},{"key":"2020112307500999000_584524v2.3","doi-asserted-by":"publisher","DOI":"10.1172\/JCI38019"},{"key":"2020112307500999000_584524v2.4","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceb.2018.05.009"},{"key":"2020112307500999000_584524v2.5","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2018.12.004"},{"key":"2020112307500999000_584524v2.6","doi-asserted-by":"crossref","first-page":"803","DOI":"10.1242\/dev.120.4.803","article-title":"Expression of inhibin subunits and follistatin during postimplantation mouse development: decidual expression of activin and expression of follistatin in primitive streak, somites and hindbrain","volume":"120","year":"1994","journal-title":"Development"},{"key":"2020112307500999000_584524v2.7","doi-asserted-by":"publisher","DOI":"10.1242\/dev.164319"},{"key":"2020112307500999000_584524v2.8","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2016.11.069"},{"key":"2020112307500999000_584524v2.9","doi-asserted-by":"crossref","first-page":"1301","DOI":"10.1242\/dev.119.4.1301","article-title":"The formation and maintenance of the definitive endoderm lineage in the mouse: involvement of HNF3\/forkhead proteins","volume":"119","year":"1993","journal-title":"Development"},{"key":"2020112307500999000_584524v2.10","doi-asserted-by":"publisher","DOI":"10.1038\/75556"},{"key":"2020112307500999000_584524v2.11","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1734197100"},{"key":"2020112307500999000_584524v2.12","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2012.08.017"},{"key":"2020112307500999000_584524v2.13","doi-asserted-by":"publisher","DOI":"10.1016\/s0925-4773(98)00015-x"},{"key":"2020112307500999000_584524v2.14","doi-asserted-by":"publisher","DOI":"10.1242\/dev.02877"},{"key":"2020112307500999000_584524v2.15","doi-asserted-by":"crossref","first-page":"1123","DOI":"10.1242\/dev.116.4.1123","article-title":"Mox-1 and Mox-2 define a novel homeobox gene subfamily and are differentially expressed during early mesodermal patterning in mouse embryos","volume":"116","year":"1992","journal-title":"Development"},{"key":"2020112307500999000_584524v2.16","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.21.23.8184-8188.2001"},{"key":"2020112307500999000_584524v2.17","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0401654101"},{"key":"2020112307500999000_584524v2.18","doi-asserted-by":"crossref","unstructured":"Dias, A. and Aires, R. (2020). Axial Stem Cells and the Formation of the Vertebrate Body. In Concepts and Applications of Stem Cell Biology. (ed. Rodrigues, G. ) and Roelen, B. A. J. ), pp. 131\u2013158. Springer, Cham.","DOI":"10.1007\/978-3-030-43939-2_8"},{"key":"2020112307500999000_584524v2.19","doi-asserted-by":"crossref","first-page":"e56615","DOI":"10.7554\/eLife.56615","article-title":"A TgfbRI\/Snai1-dependent developmental module at the core of vertebrate axial elongation","volume":"9","year":"2020","journal-title":"Elife"},{"key":"2020112307500999000_584524v2.20","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(92)90232-6"},{"key":"2020112307500999000_584524v2.21","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)90627-3"},{"key":"2020112307500999000_584524v2.22","doi-asserted-by":"publisher","DOI":"10.1242\/dev.180190"},{"key":"2020112307500999000_584524v2.23","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1001937"},{"key":"2020112307500999000_584524v2.24","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2017.04.002"},{"key":"2020112307500999000_584524v2.25","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1996.0297"},{"key":"2020112307500999000_584524v2.26","doi-asserted-by":"publisher","DOI":"10.1101\/gad.10.3.313"},{"key":"2020112307500999000_584524v2.27","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.2000.9878"},{"key":"2020112307500999000_584524v2.28","unstructured":"Hay, B. (1968). Organization and fine structure of epithelium and mesenchyme in the developing chick embryo. In Epithelial-Mesenchymal Interactions (ed. Fleischmajer, R. ) and Billingham, R. E. ), pp. 31\u201355. Philadelphia: Williams & Wilkins Co."},{"key":"2020112307500999000_584524v2.29","doi-asserted-by":"publisher","DOI":"10.1242\/dev.119768"},{"key":"2020112307500999000_584524v2.30","doi-asserted-by":"publisher","DOI":"10.1038\/343617a0"},{"key":"2020112307500999000_584524v2.31","doi-asserted-by":"publisher","DOI":"10.1242\/dev.153262"},{"key":"2020112307500999000_584524v2.32","doi-asserted-by":"publisher","DOI":"10.1016\/0925-4773(94)90098-1"},{"key":"2020112307500999000_584524v2.33","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2013.05.009"},{"key":"2020112307500999000_584524v2.34","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2017.07.021"},{"key":"2020112307500999000_584524v2.35","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.18-01-00237.1998"},{"key":"2020112307500999000_584524v2.36","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1923"},{"key":"2020112307500999000_584524v2.37","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-014-0550-8"},{"key":"2020112307500999000_584524v2.38","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2011.11.001"},{"key":"2020112307500999000_584524v2.39","doi-asserted-by":"publisher","DOI":"10.1242\/dev.022020"},{"key":"2020112307500999000_584524v2.40","doi-asserted-by":"publisher","DOI":"10.1038\/10320"},{"key":"2020112307500999000_584524v2.41","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1242\/dev.111.1.61","article-title":"Expression of the mouse labial-like homeobox-containing genes, Hox 2.9 and Hox 1.6, during segmentation of the hindbrain","volume":"111","year":"1991","journal-title":"Development"},{"key":"2020112307500999000_584524v2.42","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1007417108"},{"key":"2020112307500999000_584524v2.43","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4773(96)00653-3"},{"key":"2020112307500999000_584524v2.44","doi-asserted-by":"crossref","unstructured":"Olivera-Martinez, I. , Harada, H. , Halley, P. A. and Storey, K. G. (2012). Loss of FGF-Dependent Mesoderm Identity and Rise of Endogenous Retinoid Signalling Determine Cessation of Body Axis Elongation. PLoS Biol. 10,.","DOI":"10.1371\/journal.pbio.1001415"},{"key":"2020112307500999000_584524v2.45","doi-asserted-by":"publisher","DOI":"10.1242\/dev.078071"},{"key":"2020112307500999000_584524v2.46","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2014.006"},{"key":"2020112307500999000_584524v2.47","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-019-0933-9"},{"key":"2020112307500999000_584524v2.48","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2018.12.016"},{"key":"2020112307500999000_584524v2.49","doi-asserted-by":"crossref","first-page":"1327","DOI":"10.1002\/jor.23205","article-title":"Spatiotemporal analysis of putative notochordal cell markers reveals CD24 and keratins 8, 18, and 19 as notochord-specific markers during early human intervertebral disc development","volume":"34","year":"2016","journal-title":"J. Orthop. Res."},{"key":"2020112307500999000_584524v2.50","doi-asserted-by":"publisher","DOI":"10.1101\/gad.851501"},{"key":"2020112307500999000_584524v2.51","doi-asserted-by":"publisher","DOI":"10.1242\/dev.056622"},{"key":"2020112307500999000_584524v2.52","doi-asserted-by":"publisher","DOI":"10.1101\/gad.250603"},{"key":"2020112307500999000_584524v2.53","doi-asserted-by":"publisher","DOI":"10.1038\/5007"},{"key":"2020112307500999000_584524v2.54","doi-asserted-by":"publisher","DOI":"10.1186\/1471-213X-1-4"},{"key":"2020112307500999000_584524v2.55","doi-asserted-by":"publisher","DOI":"10.1387\/ijdb.052095cs"},{"key":"2020112307500999000_584524v2.56","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2017.01.021"},{"key":"2020112307500999000_584524v2.57","doi-asserted-by":"publisher","DOI":"10.1101\/gad.7.11.2071"},{"key":"2020112307500999000_584524v2.58","doi-asserted-by":"publisher","DOI":"10.1101\/gad.8.2.174"},{"key":"2020112307500999000_584524v2.59","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4773(02)00021-7"},{"key":"2020112307500999000_584524v2.60","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.2450"},{"key":"2020112307500999000_584524v2.61","doi-asserted-by":"publisher","DOI":"10.1242\/dev.101014"},{"key":"2020112307500999000_584524v2.62","doi-asserted-by":"publisher","DOI":"10.1242\/dev.112979"},{"key":"2020112307500999000_584524v2.63","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2009.08.002"},{"key":"2020112307500999000_584524v2.64","doi-asserted-by":"publisher","DOI":"10.1242\/dev.079848"},{"key":"2020112307500999000_584524v2.65","doi-asserted-by":"publisher","DOI":"10.1242\/dev.166215"},{"key":"2020112307500999000_584524v2.66","doi-asserted-by":"crossref","first-page":"1435","DOI":"10.1242\/dev.113.4.1435","article-title":"Pax-6, a murine paired box gene, is expressed in the developing CNS","volume":"113","year":"1991","journal-title":"Development"},{"key":"2020112307500999000_584524v2.67","doi-asserted-by":"publisher","DOI":"10.1242\/dev.039172"},{"key":"2020112307500999000_584524v2.68","doi-asserted-by":"publisher","DOI":"10.7554\/elife.10042"},{"key":"2020112307500999000_584524v2.69","doi-asserted-by":"publisher","DOI":"10.1242\/dev.168161"},{"key":"2020112307500999000_584524v2.70","doi-asserted-by":"crossref","first-page":"1211","DOI":"10.1242\/dev.126.6.1211","article-title":"A Wnt5a pathway underlies outgrowth of multiple structures in the vertebrate embryo","volume":"126","year":"1999","journal-title":"Development"}],"container-title":[],"original-title":[],"link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1101\/584524","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,14]],"date-time":"2026-01-14T22:28:51Z","timestamp":1768429731000},"score":1,"resource":{"primary":{"URL":"http:\/\/biorxiv.org\/lookup\/doi\/10.1101\/584524"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,3,21]]},"references-count":70,"URL":"https:\/\/doi.org\/10.1101\/584524","relation":{},"subject":[],"published":{"date-parts":[[2019,3,21]]},"subtype":"preprint"}}