{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T14:29:17Z","timestamp":1768487357605,"version":"3.49.0"},"posted":{"date-parts":[[2019,10,17]]},"group-title":"Developmental Biology","reference-count":94,"publisher":"openRxiv","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2019,12,31]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Somitogenesis starts with cyclic waves of expression of segmentation clock genes in the presomitic mesoderm (PSM) and culminates with periodic budding of somites in its anterior-most region. How cyclic clock gene expression is translated into timely morphological somite formation has remained unclear. A posterior to anterior gradient of increasing PSM tissue cohesion correlates with increasing fibronectin matrix complexity around the PSM, suggesting that fibronectin-dependent tissue mechanics may be involved in this transition. Here we address whether the mechanical properties of the PSM tissue play a role in regulating the pathway leading to cleft formation in the anterior PSM. We first interfered with cytoskeletal contractility in the chick PSM by disrupting actomyosin-mediated contractility directly or via Rho-associated protein kinase function. Then we perturbed fibronectin matrix accumulation around the PSM tissue by blocking integrin-fibronectin binding or fibronectin matrix assembly. All four treatments perturbed\n                  <jats:italic>hairy1<\/jats:italic>\n                  and\n                  <jats:italic>meso1<\/jats:italic>\n                  expression dynamics and resulted in defective somitic clefts. A model is presented where a gradient of fibronectin-dependent tissue mechanics participates in the PSM wavefront of maturation by ensuring the correct spatio-temporal conversion of cyclic segmentation clock gene expression into periodic somite formation.\n                <\/jats:p>","DOI":"10.1101\/808121","type":"posted-content","created":{"date-parts":[[2019,10,17]],"date-time":"2019-10-17T22:55:10Z","timestamp":1571352910000},"source":"Crossref","is-referenced-by-count":1,"title":["Fibronectin-dependent tissue mechanics regulate the translation of segmentation clock oscillations into periodic somite formation"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2369-2874","authenticated-orcid":false,"given":"Patr\u00edcia","family":"Gomes de 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