{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T13:56:44Z","timestamp":1768485404680,"version":"3.49.0"},"posted":{"date-parts":[[2019,11,18]]},"group-title":"Evolutionary Biology","reference-count":42,"publisher":"openRxiv","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2019,11,18]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Reptiles show a remarkable diversity of sex determination mechanisms and sex chromosome systems, derived from different autosomal pairs. The origin of the ZW sex chromosomes of\n                  <jats:italic>Lacerta agilis<\/jats:italic>\n                  , a widespread Eurasian lizard species, is a matter of discussion: is it a small macrochromosome from the 11-18 group, common to all lacertids, or this species has unique ZW pair derived from the large chromosome 5. Using independent molecular cytogenetic methods, we investigated the karyotype of\n                  <jats:italic>L. agilis exigua<\/jats:italic>\n                  from Siberia, Russia, to identify the sex chromosomes. FISH with the flow-sorted chromosome painting probe, derived from\n                  <jats:italic>L. strigata<\/jats:italic>\n                  and specific to chromosomes 13, 14, and Z, confirmed that the Z chromosome of\n                  <jats:italic>L. agilis<\/jats:italic>\n                  is a small macrochromosome, the same as in\n                  <jats:italic>L. strigata<\/jats:italic>\n                  . FISH with the telomeric probe showed an extensive accumulation of the telomeric repeat on the W chromosome in agreement with previous studies, excluding the possibility that the lineages of\n                  <jats:italic>L. agilis<\/jats:italic>\n                  studied in different works could have different sex chromosome systems due to a putative intra-species polymorphism. Our results reinforce the idea of the stability of the sex chromosomes and lack of evidence for sex-chromosome turnovers in known species of Lacertidae.\n                <\/jats:p>","DOI":"10.1101\/846501","type":"posted-content","created":{"date-parts":[[2019,11,18]],"date-time":"2019-11-18T18:55:12Z","timestamp":1574103312000},"source":"Crossref","is-referenced-by-count":0,"title":["Chromosome painting does not support a sex chromosome turnover in Lacerta agilis Linnaeus, 1758"],"prefix":"10.64898","author":[{"given":"Artem P.","family":"Lisachov","sequence":"first","affiliation":[]},{"given":"Massimo","family":"Giovannotti","sequence":"additional","affiliation":[]},{"given":"Jorge C.","family":"Pereira","sequence":"additional","affiliation":[]},{"given":"Daria A.","family":"Andreyushkova","sequence":"additional","affiliation":[]},{"given":"Svetlana A.","family":"Romanenko","sequence":"additional","affiliation":[]},{"given":"Malcolm A.","family":"Ferguson-Smith","sequence":"additional","affiliation":[]},{"given":"Pavel M.","family":"Borodin","sequence":"additional","affiliation":[]},{"given":"Vladimir A.","family":"Trifonov","sequence":"additional","affiliation":[]}],"member":"54368","reference":[{"key":"2019112013350662000_846501v2.1","doi-asserted-by":"publisher","DOI":"10.1038\/nature10390"},{"key":"2019112013350662000_846501v2.2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1820320116"},{"key":"2019112013350662000_846501v2.3","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1163\/156853894X00506","article-title":"Mechanisms of differentiation in the sex chromosomes of some Lacertidae","volume":"15","year":"1994","journal-title":"Amphibia-Reptilia"},{"key":"2019112013350662000_846501v2.4","first-page":"73","article-title":"Description of the orcein stained karyotypes of 36 lizard species (Lacertilia, Reptilia) belonging to the families Teiidae, Scincidae, Lacertidae, Cordylidae and Varanidae 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