{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T19:43:06Z","timestamp":1776195786565,"version":"3.50.1"},"reference-count":52,"publisher":"Cold Spring Harbor Laboratory","issue":"12","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes Dev."],"published-print":{"date-parts":[[1998,6,15]]},"abstract":"<jats:p>Allelic exclusion in \u03ba light-chain synthesis is thought to result from a feedback mechanism by which the expression of a functional \u03ba light chain on the surface of the B cell leads to an intracellular signal that down-regulates the <jats:italic>V(D)J<\/jats:italic> recombinase, thus precluding rearrangement of the other allele. Whereas such a feedback mechanism clearly plays a role in the maintenance of allelic exclusion, here we provide evidence suggesting that the initial establishment of allelic exclusion involves differential availability of the two \u03ba alleles for rearrangement. Analysis of \u03ba<jats:sup>+<\/jats:sup> B-cell populations and of individual \u03ba<jats:sup>+<\/jats:sup> B cells that have rearranged only one allele demonstrates that in these cells, critical sites on the rearranged allele are unmethylated, whereas the nonrearranged allele remains methylated. This pattern is apparently generated by demethylation that is initiated at the small pre-B cell stage, on a single allele, in a process that occurs prior to rearrangement and requires the presence in <jats:italic>cis<\/jats:italic> of both the intronic and 3\u2032 \u03ba enhancers. Taken together with data demonstrating that undermethylation is required for rearrangement, these results indicate that demethylation may actually underly the process of allelic exclusion by directing the initial choice of a single \u03ba allele for rearrangement.<\/jats:p>","DOI":"10.1101\/gad.12.12.1801","type":"journal-article","created":{"date-parts":[[2008,2,20]],"date-time":"2008-02-20T22:52:17Z","timestamp":1203547937000},"page":"1801-1811","source":"Crossref","is-referenced-by-count":154,"title":["\u03ba chain monoallelic demethylation and the establishment of allelic\u2009exclusion"],"prefix":"10.1101","volume":"12","author":[{"given":"Raul","family":"Mostoslavsky","sequence":"first","affiliation":[]},{"given":"Nandita","family":"Singh","sequence":"additional","affiliation":[]},{"given":"Andrei","family":"Kirillov","sequence":"additional","affiliation":[]},{"given":"Roberta","family":"Pelanda","sequence":"additional","affiliation":[]},{"given":"Howard","family":"Cedar","sequence":"additional","affiliation":[]},{"given":"Andrew","family":"Chess","sequence":"additional","affiliation":[]},{"given":"Yehudit","family":"Bergman","sequence":"additional","affiliation":[]}],"member":"246","published-online":{"date-parts":[[1998,6,15]]},"reference":[{"key":"2021111418543457000_12.12.1801.1","first-page":"616","article-title":"Detection of DNA in single cells using an automated cell deposition unit and PCR.","volume":"19","author":"Bertram","year":"1995","journal-title":"BioTechniques"},{"key":"2021111418543457000_12.12.1801.2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(94)90316-6"},{"key":"2021111418543457000_12.12.1801.3","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.15.7871"},{"key":"2021111418543457000_12.12.1801.4","doi-asserted-by":"crossref","first-page":"4335","DOI":"10.1002\/j.1460-2075.1993.tb06118.x","article-title":"TCR\u03b2 and TCR\u03b1 gene enhancers confer tissue- and stage-specificity on V(D)J recombination events.","volume":"12","author":"Capone","year":"1993","journal-title":"EMBO J."},{"key":"2021111418543457000_12.12.1801.5","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(00)80673-1"},{"key":"2021111418543457000_12.12.1801.6","doi-asserted-by":"crossref","first-page":"4635","DOI":"10.1002\/j.1460-2075.1993.tb06152.x","article-title":"Mutations of the intronic IgH enhancer and its flanking sequences differentially affect accessibility of the JH locus.","volume":"12","author":"Chen","year":"1993","journal-title":"EMBO J."},{"key":"2021111418543457000_12.12.1801.7","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(94)90562-2"},{"key":"2021111418543457000_12.12.1801.8","doi-asserted-by":"publisher","DOI":"10.1038\/290372a0"},{"key":"2021111418543457000_12.12.1801.9","doi-asserted-by":"publisher","DOI":"10.1084\/jem.185.4.609"},{"key":"2021111418543457000_12.12.1801.10","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/7.12.1995"},{"key":"2021111418543457000_12.12.1801.11","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)90398-A"},{"key":"2021111418543457000_12.12.1801.12","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(91)90352-Y"},{"key":"2021111418543457000_12.12.1801.13","doi-asserted-by":"crossref","first-page":"3217","DOI":"10.1128\/MCB.15.6.3217","article-title":"The V(D)J recombinational and transcriptional activities of the immunoglobulin heavy-chain intronic enhancer can be mediated through distinct protein-binding sites in a transgenic substrate.","volume":"15","author":"Fernex","year":"1995","journal-title":"Mol. 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