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In multi-intron precursor RNAs containing duplicated splice sites, mutation of the poly(A) site inhibited removal of the last intron, but not the removal of introns farther upstream. Inhibition of removal of only the last intron suggests segmental recognition of multi-exon precursor RNAs and is consistent with previous suggestions that signals at both ends of an exon are required for effective splicing of an upstream intron.<\/jats:p>","DOI":"10.1101\/gad.5.11.2086","type":"journal-article","created":{"date-parts":[[2007,6,5]],"date-time":"2007-06-05T21:15:46Z","timestamp":1181078146000},"page":"2086-2095","source":"Crossref","is-referenced-by-count":156,"title":["Mutation of the AAUAAA polyadenylation signal depresses in vitro splicing of proximal but not distal introns."],"prefix":"10.1101","volume":"5","author":[{"given":"M","family":"Niwa","sequence":"first","affiliation":[]},{"given":"S M","family":"Berget","sequence":"additional","affiliation":[]}],"member":"246","published-online":{"date-parts":[[1991,11,1]]},"reference":[{"key":"2021111320200929000_5.11.2086.1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(86)90620-3"},{"key":"2021111320200929000_5.11.2086.2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(79)90468-6"},{"key":"2021111320200929000_5.11.2086.3","doi-asserted-by":"publisher","DOI":"10.1101\/gad.2.6.754"},{"key":"2021111320200929000_5.11.2086.4","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(88)91263-9"},{"key":"2021111320200929000_5.11.2086.5","doi-asserted-by":"crossref","first-page":"1747","DOI":"10.1002\/j.1460-2075.1987.tb02427.x","article-title":"Two spliceosomes can form simultaneously and independently on synthetic double-intron messenger RNA precursors.","volume":"6","year":"1987","journal-title":"EMBO J."},{"key":"2021111320200929000_5.11.2086.6","doi-asserted-by":"crossref","first-page":"795","DOI":"10.1128\/jvi.28.3.795-801.1978","article-title":"Transcription pattern of in vivo-labeled late simian virus 40 RNA: Equimolar transcription beyond the mRNA 3\u2032 terminus.","volume":"28","year":"1978","journal-title":"J. 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