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We show that eve autoregulation is mediated, at least in part, by a 100-bp minimal autoregulatory sequence (MAS) located approximately 5 kb upstream from the eve transcription start site. Multimerization of a 200-bp DNA fragment that encompasses the MAS drives optimal autoregulatory activity, comparable to that obtained with the native distal enhancer element located between -5.9 and -5.2 kb. The MAS contains two eve protein-binding sites, as well as binding sites for two nuclear factors present in early embryos. Directed mutagenesis of these binding sites suggests that both the eve protein and nuclear factors are essential for autoregulation. These results provide evidence that the eve protein acts combinatorially with other transcription factors to enhance its own expression.<\/jats:p>","DOI":"10.1101\/gad.5.2.265","type":"journal-article","created":{"date-parts":[[2007,6,5]],"date-time":"2007-06-05T21:15:46Z","timestamp":1181078146000},"page":"265-277","source":"Crossref","is-referenced-by-count":108,"title":["Autoregulation of a segmentation gene in Drosophila: combinatorial interaction of the even-skipped homeo box protein with a distal enhancer element."],"prefix":"10.1101","volume":"5","author":[{"given":"J","family":"Jiang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T","family":"Hoey","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Levine","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"246","published-online":{"date-parts":[[1991,2,1]]},"reference":[{"key":"2021111320162626000_5.2.265.1","doi-asserted-by":"publisher","DOI":"10.1038\/msb.2012.72"},{"key":"2021111320162626000_5.2.265.2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(88)90088-8"},{"key":"2021111320162626000_5.2.265.3","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(89)90424-8"},{"key":"2021111320162626000_5.2.265.4","doi-asserted-by":"crossref","first-page":"779","DOI":"10.1002\/j.1460-2075.1987.tb04820.x","article-title":"Temporal and spatial distribution of transcripts from the Deformed gene of Drosophila.","volume":"6","year":"1987","journal-title":"EMBO J."},{"key":"2021111320162626000_5.2.265.5","doi-asserted-by":"publisher","DOI":"10.1038\/340363a0"},{"key":"2021111320162626000_5.2.265.6","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1.9.981"},{"key":"2021111320162626000_5.2.265.7","doi-asserted-by":"crossref","first-page":"749","DOI":"10.1002\/j.1460-2075.1987.tb04817.x","article-title":"Characterization and localization of the even-skipped protein of Drosophila.","volume":"6","year":"1987","journal-title":"EMBO J."},{"key":"2021111320162626000_5.2.265.8","doi-asserted-by":"publisher","DOI":"10.1101\/gad.2.12b.1824"},{"key":"2021111320162626000_5.2.265.9","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(89)90916-1"},{"key":"2021111320162626000_5.2.265.10","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(89)90580-1"},{"key":"2021111320162626000_5.2.265.11","doi-asserted-by":"publisher","DOI":"10.1126\/science.3755551"},{"key":"2021111320162626000_5.2.265.12","doi-asserted-by":"crossref","first-page":"1205","DOI":"10.1002\/j.1460-2075.1989.tb03493.x","article-title":"Auto-regulatory and gap response elements of the even-skipped promoter of Drosophila.","volume":"8","year":"1989","journal-title":"EMBO J."},{"key":"2021111320162626000_5.2.265.13","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1002\/j.1460-2075.1990.tb08097.x","article-title":"The tramtrack gene in Drosophila encodes a zinc finger protein that interacts with the ftz transcriptional regulatory region and shows a novel embryonic expression pattern.","volume":"9","year":"1990","journal-title":"EMBO J."},{"key":"2021111320162626000_5.2.265.14","first-page":"956","article-title":"Characterization of Drosophila transcription factors that activate the tandem promoters of the alcohol dehydrogenase gene.","volume":"41","year":"1985","journal-title":"Cell"},{"key":"2021111320162626000_5.2.265.15","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(87)90523-X"},{"key":"2021111320162626000_5.2.265.16","doi-asserted-by":"publisher","DOI":"10.1038\/332858a0"},{"key":"2021111320162626000_5.2.265.17","first-page":"4598","article-title":"The DNA binding activities of the Drosophila even-skipped protein are mediated by its homeo domain and influenced by protein context.","volume":"8","year":"1988","journal-title":"Mol. 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