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Cold-insensitive growth can also be restored by two classes of recessive (loss-of-function) suppressors: (1) mutations in PRP24, which we show to be a U6-specific binding protein of the RNP-consensus family; and (2) mutations in U6, which lie outside the interaction domain and identify putative PRP24-binding sites. Destabilization of the U4\/U6 helix causes the accumulation of a PRP24\/U4\/U6 complex, which is undetectable in wild-type cells. The loss-of-function suppressor mutations inhibit the binding of PRP24 to U6, and thus presumably promote the release of PRP24 from the PRP24\/U4\/U6 complex and the reformation of the base-paired U4\/U6 snRNP. We propose that the PRP24\/U4\/U6 complex is normally a highly transient intermediate in the spliceosome cycle and that PRP24 promotes the reannealing of U6 with U4.<\/jats:p>","DOI":"10.1101\/gad.5.5.773","type":"journal-article","created":{"date-parts":[[2007,6,5]],"date-time":"2007-06-05T21:15:46Z","timestamp":1181078146000},"page":"773-785","source":"Crossref","is-referenced-by-count":106,"title":["Suppressors of a U4 snRNA mutation define a novel U6 snRNP protein with RNA-binding motifs."],"prefix":"10.1101","volume":"5","author":[{"given":"K W","family":"Shannon","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C","family":"Guthrie","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"246","published-online":{"date-parts":[[1991,5,1]]},"reference":[{"key":"2021111320173540000_5.5.773.1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.3.4.431"},{"key":"2021111320173540000_5.5.773.2","doi-asserted-by":"crossref","first-page":"2415","DOI":"10.1002\/j.1460-2075.1987.tb02520.x","article-title":"An ordered pathway of snRNP binding during mammalian pre-mRNA splicing complex assembly.","volume":"6","year":"1987","journal-title":"EMBO J."},{"key":"2021111320173540000_5.5.773.3","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1002\/j.1460-2075.1990.tb08102.x","article-title":"Discrete domains of human U6 snRNA required for the assembly of U4\/U6 snRNP and splicing complexes.","volume":"9","year":"1990","journal-title":"EMBO J."},{"key":"2021111320173540000_5.5.773.4","first-page":"3350","article-title":"U5 small nuclear ribonucleoprotein: RNA structure analysis and ATP-dependent interaction with U4\/U6.","volume":"9","year":"1989","journal-title":"Mol. 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