{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T22:20:44Z","timestamp":1774995644394,"version":"3.50.1"},"reference-count":52,"publisher":"Cold Spring Harbor Laboratory","issue":"21","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes Dev."],"published-print":{"date-parts":[[1995,11,1]]},"abstract":"<jats:p>Fibroblast growth factor 4 (FGF-4) has been shown to be a signaling molecule whose expression is essential for postimplantation mouse development and, at later embryonic stages, for limb patterning and growth. The FGF-4 gene is expressed in the blastocyst inner cell mass and later in distinct embryonic tissues but is transcriptionally silent in the adult. In tissue culture FGF-4 expression is restricted to undifferentiated embryonic stem (ES) cells and embryonal carcinoma (EC) cell lines. Previously, we determined that EC cell-specific transcriptional activation of the FGF-4 gene depends on a synergistic interaction between octamer-binding proteins and an EC-specific factor, Fx, that bind adjacent sites on the FGF-4 enhancer. Through the cloning and characterization of an F9 cell cDNA we now show that the latter activity is Sox2, a member of the Sry-related Sox factors family. Sox2 can form a ternary complex with either the ubiquitous Oct-1 or the embryonic-specific Oct-3 protein on FGF-4 enhancer DNA sequences. However, only the Sox2\/Oct-3 complex is able to promote transcriptional activation. These findings identify FGF-4 as the first known embryonic target gene for Oct-3 and for any of the Sox factors, and offer insights into the mechanisms of selective gene activation by Sox and octamer-binding proteins during embryogenesis.<\/jats:p>","DOI":"10.1101\/gad.9.21.2635","type":"journal-article","created":{"date-parts":[[2007,6,5]],"date-time":"2007-06-05T21:15:46Z","timestamp":1181078146000},"page":"2635-2645","source":"Crossref","is-referenced-by-count":606,"title":["Developmental-specific activity of the FGF-4 enhancer requires the synergistic action of Sox2 and Oct-3."],"prefix":"10.1101","volume":"9","author":[{"given":"H","family":"Yuan","sequence":"first","affiliation":[]},{"given":"N","family":"Corbi","sequence":"additional","affiliation":[]},{"given":"C","family":"Basilico","sequence":"additional","affiliation":[]},{"given":"L","family":"Dailey","sequence":"additional","affiliation":[]}],"member":"246","published-online":{"date-parts":[[1995,11,1]]},"reference":[{"key":"2021111418261353000_9.21.2635.1","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/S0065-230X(08)60305-X","article-title":"The FGF family of growth factors and oncogenes.","volume":"59","year":"1992","journal-title":"Adv. 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