{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T03:40:21Z","timestamp":1761968421275,"version":"build-2065373602"},"reference-count":5,"publisher":"Cold Spring Harbor Laboratory","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cold Spring Harb Protoc"],"published-print":{"date-parts":[[2012,1]]},"abstract":"<jats:p>The <jats:italic>Drosophila<\/jats:italic> cuticle carries a rich array of morphological details. Thus, cuticle examination has had a central role in the history of genetics. This protocol describes a procedure for mounting adult cuticles in Hoyer\u2019s medium, a useful mountant for both larval and adult cuticles. The medium digests soft tissues rapidly, leaving the cuticle cleared for observation. In addition, samples can be transferred directly from water to Hoyer\u2019s medium. However, specimens mounted in Hoyer\u2019s medium degrade over time. For example, the fine denticles on the larval dorsum are best observed soon after mounting; they begin to fade after 1 week, and can disappear completely after several months. More robust features, such as the ventral denticle belts, will persist for a longer period of time. Because adults cannot profitably be mounted whole in Hoyer\u2019s medium, some dissection is necessary.<\/jats:p>","DOI":"10.1101\/pdb.prot067371","type":"journal-article","created":{"date-parts":[[2011,12,22]],"date-time":"2011-12-22T17:47:21Z","timestamp":1324576041000},"page":"pdb.prot067371","source":"Crossref","is-referenced-by-count":4,"title":["Rapid Mounting of Adult <i>Drosophila<\/i> Structures in Hoyer\u2019s Medium"],"prefix":"10.1101","volume":"2012","author":[{"given":"David L.","family":"Stern","sequence":"first","affiliation":[]},{"given":"Elio","family":"Sucena","sequence":"additional","affiliation":[]}],"member":"246","published-online":{"date-parts":[[2011,12,22]]},"reference":[{"key":"2021111008280164000_2012.1.pdb.prot067371.1","first-page":"1","article-title":"A phylogenetic, revised classification of genera in the Drosophilidae (Diptera)","volume":"197","year":"1990","journal-title":"Bull Am Mus Nat Hist"},{"key":"2021111008280164000_2012.1.pdb.prot067371.2","first-page":"337","article-title":"Scanning electron microscopy of the adult of Drosophila melanogaster","volume-title":"The genetics and biology of Drosophila","volume":"vol. 2c","year":"1978"},{"key":"2021111008280164000_2012.1.pdb.prot067371.3","first-page":"218","article-title":"Cuticle morphology changes with each larval molt in D. melanogaster","volume":"71","year":"1992","journal-title":"Dros Info Services"},{"key":"2021111008280164000_2012.1.pdb.prot067371.4","doi-asserted-by":"publisher","DOI":"10.1101\/pdb.prot065532"},{"key":"2021111008280164000_2012.1.pdb.prot067371.5","doi-asserted-by":"publisher","DOI":"10.1101\/pdb.prot066498"}],"container-title":["Cold Spring Harbor Protocols"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1101\/pdb.prot067371","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,10]],"date-time":"2021-11-10T18:29:56Z","timestamp":1636568996000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.cshprotocols.org\/lookup\/doi\/10.1101\/pdb.prot067371"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,12,22]]},"references-count":5,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2011,12,22]]},"published-print":{"date-parts":[[2012,1]]}},"alternative-id":["10.1101\/pdb.prot067371"],"URL":"https:\/\/doi.org\/10.1101\/pdb.prot067371","relation":{},"ISSN":["1940-3402","1559-6095"],"issn-type":[{"type":"print","value":"1940-3402"},{"type":"electronic","value":"1559-6095"}],"subject":[],"published":{"date-parts":[[2011,12,22]]}}}