{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T01:40:55Z","timestamp":1789522855248,"version":"build-2803163510"},"reference-count":37,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2002,9,1]],"date-time":"2002-09-01T00:00:00Z","timestamp":1030838400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2002,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Plants can grow in soils containing highly variable amounts of mineral nutrients, like Ca2+ and Mn2+, though the mechanisms of adaptation are poorly understood. Here, we report the first genetic study to determine in vivo functions of a Ca2+ pump in plants. Homozygous mutants of Arabidopsis harboring a T-DNA disruption in ECA1 showed a 4-fold reduction in endoplasmic reticulum-type calcium pump activity. Surprisingly, the phenotype of mutant plants was indistinguishable from wild type when grown on standard nutrient medium containing 1.5 mmCa2+ and 50 \u03bcm Mn2+. However, mutants grew poorly on medium with low Ca2+ (0.2 mm) or high Mn2+ (0.5 mm). On high Mn2+, the mutants failed to elongate their root hairs, suggesting impairment in tip growth processes. Expression of the wild-type gene (CAMV35S::ECA1) reversed these conditional phenotypes. The activity of ECA1 was examined by expression in a yeast (Saccharomyces cerevisiae) mutant, K616, which harbors a deletion of its endogenous calcium pumps. In vitro assays demonstrated that Ca2+, Mn2+, and Zn2+stimulated formation of a phosphoenzyme intermediate, consistent with the translocation of these ions by the pump. ECA1 provided increased tolerance of yeast mutant to toxic levels of Mn2+ (1 mm) and Zn2+(3 mm), consistent with removal of these ions from the cytoplasm. These results show that despite the potential redundancy of multiple Ca2+ pumps and Ca2+\/H+ antiporters in Arabidopsis, pumping of Ca2+ and Mn2+ by ECA1 into the endoplasmic reticulum is required to support plant growth under conditions of Ca2+ deficiency or Mn2+ toxicity.<\/jats:p>","DOI":"10.1104\/pp.004440","type":"journal-article","created":{"date-parts":[[2002,9,11]],"date-time":"2002-09-11T21:28:03Z","timestamp":1031779683000},"page":"128-137","source":"Crossref","is-referenced-by-count":157,"title":["An Endoplasmic Reticulum-Bound Ca2+\/Mn2+Pump, ECA1, Supports Plant Growth and Confers Tolerance to Mn2+ Stress"],"prefix":"10.1093","volume":"130","author":[{"given":"Zhongyi","family":"Wu","sequence":"first","affiliation":[{"name":"Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, Maryland 20742 (Z.W., F.L., H.S.);"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, Maryland 20742 (Z.W., F.L., H.S.);"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bimei","family":"Hong","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Plant Division, The Scripps Research Institute, La Jolla, California 92037 (B.H., J.F.H.); and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeff C.","family":"Young","sequence":"additional","affiliation":[{"name":"Biotechnology Center, 425 Henry Mall, University of Wisconsin, Madison, Wisconsin 53706 (J.C.Y., M.R.S.)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael R.","family":"Sussman","sequence":"additional","affiliation":[{"name":"Biotechnology Center, 425 Henry Mall, University of Wisconsin, Madison, Wisconsin 53706 (J.C.Y., M.R.S.)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey F.","family":"Harper","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Plant Division, The Scripps Research Institute, La Jolla, California 92037 (B.H., J.F.H.); and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heven","family":"Sze","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, Maryland 20742 (Z.W., F.L., H.S.);"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2002,9,1]]},"reference":[{"key":"2021062513071460500_R1","doi-asserted-by":"crossref","first-page":"696","DOI":"10.1104\/pp.126.2.696","article-title":"Inventory of the superfamily of P-type ion pumps in Arabidopsis.","volume":"126","author":"Axelsen","year":"2001","journal-title":"Plant Physiol"},{"key":"2021062513071460500_R2","first-page":"1194","article-title":"In planta Agrobacterium mediated gene transfer by infiltration of adult Arabidopsis thaliana plants.","volume":"316","author":"Bechtold","year":"1993","journal-title":"C R Acad Sci Paris Life Sci"},{"key":"2021062513071460500_R3","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1007\/s004250050219","article-title":"Root hair growth in Arabidopsis thaliana is directed by calcium and an endogenous polarity.","volume":"203","author":"Bibikova","year":"1997","journal-title":"Planta"},{"key":"2021062513071460500_R4","doi-asserted-by":"crossref","first-page":"1495","DOI":"10.1104\/pp.123.4.1495","article-title":"At-ACA8 encodes a plasma membrane-localized calcium-ATPase of Arabidopsis with a calmodulin-binding domain at the N terminus.","volume":"123","author":"Bonza","year":"2000","journal-title":"Plant Physiol"},{"key":"2021062513071460500_R5","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1007\/978-94-009-2817-6_8","article-title":"The uptake and translocation of manganese by plant roots.","volume-title":"Manganese in Soils and Plants.","author":"Clarkson","year":"1988"},{"key":"2021062513071460500_R6","doi-asserted-by":"crossref","first-page":"476","DOI":"10.1073\/pnas.81.2.476","article-title":"Mn2+ and Co2+ toxicity in chlorophyll biosynthesis.","volume":"81","author":"Csatorday","year":"1984","journal-title":"Proc Natl Acad Sci USA"},{"key":"2021062513071460500_R7","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1083\/jcb.124.3.351","article-title":"Calcineurin-dependent growth control in Saccharomyces cerevisiae mutants lacking PMC1, a homolog of plasma membrane Ca2+ ATPases.","volume":"124","author":"Cunningham","year":"1994","journal-title":"J Cell Biol"},{"key":"2021062513071460500_R8","doi-asserted-by":"crossref","first-page":"1149","DOI":"10.1091\/mbc.9.5.1149","article-title":"The medial-Golgi ion pump Pmr1 supplies the yeast secretory pathway with Ca2+ and Mn2+ required for glycosylation, sorting, and endoplasmic reticulum-associated protein degradation.","volume":"9","author":"Durr","year":"1998","journal-title":"Mol Biol Cell"},{"key":"2021062513071460500_R9","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1080\/01904169509364931","article-title":"Differential Mn tolerance of cotton genotypes in nutrient solution.","volume":"18","author":"Foy","year":"1995","journal-title":"J Plant Nutr"},{"key":"2021062513071460500_R10","doi-asserted-by":"crossref","first-page":"1814","DOI":"10.1104\/pp.124.4.1814","article-title":"The ACA4 gene of Arabidopsis encodes a vacuolar membrane calcium pump that improves salt tolerance in yeast.","volume":"124","author":"Geisler","year":"2000","journal-title":"Plant Physiol"},{"key":"2021062513071460500_R11","doi-asserted-by":"crossref","first-page":"1099","DOI":"10.1074\/jbc.273.2.1099","article-title":"A novel calmodulin-regulated Ca2+-ATPase (ACA2) from Arabidopsis with an N-terminal autoinhibitory domain.","volume":"273","author":"Harper","year":"1998","journal-title":"J Biol Chem"},{"key":"2021062513071460500_R12","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1146\/annurev.pp.36.060185.002145","article-title":"Calcium and plant development.","volume":"36","author":"Hepler","year":"1985","journal-title":"Annu Rev Plant Physiol"},{"key":"2021062513071460500_R13","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1104\/pp.124.1.125","article-title":"Expression of Arabidopsis CAX2 in tobacco. 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