{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T20:57:22Z","timestamp":1789937842817,"version":"4.0.1"},"reference-count":49,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2002,4,1]],"date-time":"2002-04-01T00:00:00Z","timestamp":1017619200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2002,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Two cDNAs encoding functional carbonic anhydrase (CA) enzymes were recently isolated from a non-photosynthetic, cotyledon library of cotton (Gossypium hirsutum) seedlings with putative plastid-targeting sequences (GenBank accession nos. AF132854 andAF132855). Relative CA transcript abundance and enzyme activity increased 9 and 15 times, respectively, in cotton embryos during the maximum period of reserve oil accumulation. Specific sulfonamide inhibitors of CA activity significantly reduced the rate of [14C]acetate incorporation into total lipids in cotton embryos in vivo, and in embryo plastids in vitro, suggesting a role for CA in plastid lipid biosynthesis. CA inhibitors did not affect acetyl-coenzyme A carboxylase activity or total storage protein synthesis. Similar results were obtained for two other plant systems: cell suspensions (and isolated plastids therefrom) of tobacco (Nicotiana tabacum), and chloroplasts isolated from leaves of transgenic CA antisense-suppressed tobacco plants (5% of wild-type CA activity). In addition, tobacco cell suspensions treated with the CA inhibitor ethoxyzolamide showed a substantial loss of CO2 compared with controls. The rate of [14C]acetate incorporation into lipid in cell suspensions was reduced by limiting external [CO2] (scrubbed air), and this rate was further reduced in the presence of ethoxyzolamide. Together, these results indicate that a reduction of CA activity (biochemical or molecular inhibition) impacts the rate of plant lipid biosynthesis from acetate, perhaps by impairing the ability of CA to efficiently \u201ctrap\u201d inorganic carbon inside plastids for utilization by acetyl-coenzyme A carboxylase and the fatty acid synthesis machinery.<\/jats:p>","DOI":"10.1104\/pp.010879","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T05:01:38Z","timestamp":1027746098000},"page":"1417-1427","source":"Crossref","is-referenced-by-count":65,"title":["Biochemical and Molecular Inhibition of Plastidial Carbonic Anhydrase Reduces the Incorporation of Acetate into Lipids in Cotton Embryos and Tobacco Cell Suspensions and Leaves"],"prefix":"10.1093","volume":"128","author":[{"given":"Chau V.","family":"Hoang","sequence":"first","affiliation":[{"name":"Department of Biological Sciences, Division of Biochemistry and Molecular Biology, University of North Texas, Denton, Texas 76203"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kent D.","family":"Chapman","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Division of Biochemistry and Molecular Biology, University of North Texas, Denton, Texas 76203"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2002,4,1]]},"reference":[{"key":"2021062512453770700_R1","doi-asserted-by":"crossref","first-page":"796","DOI":"10.1038\/35048692","article-title":"Analysis of the genome sequence of the flowering plant Arabidopsis thaliana.","volume":"408","author":"Arabidopsis Genome Initiative","year":"2000","journal-title":"Nature"},{"key":"2021062512453770700_R2","doi-asserted-by":"crossref","first-page":"1408","DOI":"10.1073\/pnas.69.6.1408","article-title":"Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.","volume":"69","author":"Aviv","year":"1972","journal-title":"Proc Natl Acad Sci"},{"key":"2021062512453770700_R3","doi-asserted-by":"crossref","first-page":"606","DOI":"10.1111\/j.1399-3054.1992.tb04711.x","article-title":"The CO2 concentrating mechanism in cyanobacteria and green algae.","volume":"84","author":"Badger","year":"1992","journal-title":"Physiol Plant"},{"key":"2021062512453770700_R4","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1146\/annurev.pp.45.060194.002101","article-title":"The role of carbonic anhydrase in photosynthesis.","volume":"45","author":"Badger","year":"1994","journal-title":"Annu Rev Plant Physiol Plant Mol Biol"},{"key":"2021062512453770700_R5","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.","volume":"12","author":"Bradford","year":"1976","journal-title":"Anal Biochem"},{"key":"2021062512453770700_R6","first-page":"148","article-title":"The Zucker-fatty rat: a review.","volume":"36","author":"Bray","year":"1977","journal-title":"Federation Proc"},{"key":"2021062512453770700_R7","doi-asserted-by":"crossref","first-page":"576","DOI":"10.1016\/0006-3002(61)90418-8","article-title":"A comment on the spectrophotometric determination of chlorophyll.","volume":"52","author":"Bruinsma","year":"1961","journal-title":"Biochem Biohys Acta"},{"key":"2021062512453770700_R8","doi-asserted-by":"crossref","first-page":"1252","DOI":"10.1104\/pp.86.4.1252","article-title":"Low bundle sheath carbonic anhydrase is apparently essential for effective C4 pathway operation.","volume":"86","author":"Burnell","year":"1988","journal-title":"Plant Physiol"},{"key":"2021062512453770700_R9","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1016\/0304-3940(91)90472-6","article-title":"Immunostaining of carbamoylphosphate synthase II and fatty acid synthase in glial cells in rat, mouse, and hamster brains suggests roles for carbonic anhydrase in biosynthetic processes.","volume":"129","author":"Cammer","year":"1991","journal-title":"Neurosci Lett"},{"key":"2021062512453770700_R10","doi-asserted-by":"crossref","first-page":"2067","DOI":"10.1016\/0024-3205(78)90449-6","article-title":"Inhibitory effect of acetazolamide on the activity of acetyl-CoA carboxylase of mouse liver.","volume":"22","author":"Cao","year":"1978","journal-title":"Life Sci"},{"key":"2021062512453770700_R11","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1111\/j.1399-3054.1995.tb00817.x","article-title":"Increased N-acylphosphatidyethanolamine biosynthesis in elicitor treated tobacco cells.","volume":"95","author":"Chapman","year":"1995","journal-title":"Physiol Plant"},{"key":"2021062512453770700_R12","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1006\/abbi.1993.1110","article-title":"N-Acylphosphatidylethanolamine synthesis in plants: occurrence, molecular composition and phospholipid origin.","volume":"301","author":"Chapman","year":"1993","journal-title":"Arch Biochem Biophys"},{"key":"2021062512453770700_R13","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1016\/S0176-1617(96)80121-0","article-title":"Developmental, tissue-specific, and environmental factors regulate the biosynthesis of N-acyl-phosphatidylethanolamine in cotton (Gossypium hirsutum L.).","volume":"149","author":"Chapman","year":"1996","journal-title":"J Plant Physiol"},{"key":"2021062512453770700_R14","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1104\/pp.62.1.141","article-title":"Control of enzyme activities in cotton cotyledons during maturation and germination: II. Gloxysomal enzyme development in embryos.","volume":"62","author":"Choinski","year":"1978","journal-title":"Plant Physiol"},{"key":"2021062512453770700_R15","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1046\/j.1365-313X.1997.11030407.x","article-title":"A carbonic anhyrdrase gene is induced in the nodule primordium and its cell-specific expression is controlled by presence of Rhizobium during development.","volume":"11","author":"Coba de la Pena","year":"1997","journal-title":"Plant J"},{"key":"2021062512453770700_R16","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1016\/S0076-6879(83)91039-X","article-title":"Radiochemical sequence analysis of metabolically labeled proteins.","volume":"91","author":"Coligan","year":"1983","journal-title":"Methods Enzymol"},{"key":"2021062512453770700_R17","doi-asserted-by":"crossref","first-page":"516","DOI":"10.1111\/j.1749-6632.1984.tb12380.x","article-title":"The role of carbonic anhydrase in hepatocyte metabolism.","volume":"429","author":"Dodgson","year":"1984","journal-title":"Ann NY Acad Sci"},{"key":"2021062512453770700_R18","doi-asserted-by":"crossref","first-page":"767","DOI":"10.1104\/pp.122.3.767","article-title":"Coordinate changes in carbon partitioning and plastidial metabolism during the development of oilseed rape embryos.","volume":"122","author":"Eastmond","year":"2000","journal-title":"Plant Physiol"},{"key":"2021062512453770700_R19","doi-asserted-by":"crossref","first-page":"1059","DOI":"10.1104\/pp.124.3.1059","article-title":"Oxygen regulation of a nodule-located carbonic anhydrase in alfalfa.","volume":"124","author":"Galvez","year":"2000","journal-title":"Plant Physiol"},{"key":"2021062512453770700_R20","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1104\/pp.93.2.825","article-title":"Carbonic anhydrase activity in leaves and its role in the first step of C4 photosynthesis.","volume":"93","author":"Hatch","year":"1990","journal-title":"Plant Physiol"},{"key":"2021062512453770700_R21","first-page":"425","article-title":"A role for carbonic anhydrase in de novo fatty acid synthesis in liver.","volume":"429","author":"Herbert","year":"1983","journal-title":"Ann NY Acad Sci"},{"key":"2021062512453770700_R22","doi-asserted-by":"crossref","first-page":"1262","DOI":"10.1093\/oxfordjournals.pcp.a029514","article-title":"Identification and expression of cotton (Gossypium hirsutum L.) plastidial carbonic anhydrase.","volume":"40","author":"Hoang","year":"1999","journal-title":"Plant Cell Physiol"},{"key":"2021062512453770700_R23","volume-title":"Molecular identification and expression of a plastidial carbonic anhyrase in cotton (Gossypium hirsutum L.) (abstract no. 51). Plant Physiology Meeting, August, 1998.","author":"Hoang","year":"1998"},{"key":"2021062512453770700_R24","doi-asserted-by":"crossref","first-page":"468","DOI":"10.1104\/pp.55.3.468","article-title":"Carbonic anhydrase of spinach: studies on its location, inhibition and physiological function.","volume":"55","author":"Jacobson","year":"1975","journal-title":"Plant Physiol"},{"key":"2021062512453770700_R25","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1094\/MPMI.2000.13.1.14","article-title":"Carbon metabolism in developing soybean root nodules: the role of carbonic anhydrase.","volume":"13","author":"Kavroulakis","year":"2000","journal-title":"Mol Plant-Microbe Interact"},{"key":"2021062512453770700_R26","doi-asserted-by":"crossref","first-page":"1071","DOI":"10.1104\/pp.117.3.1071","article-title":"Expression of tobacco carbonic anhydrase in the C4 dicot Flaveria bidentis leads to increased leakiness of the bundle sheath and a defective CO2-concentrating mechanism.","volume":"117","author":"Ludwig","year":"1998","journal-title":"Plant Physiol"},{"key":"2021062512453770700_R27","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1042\/bj3100197","article-title":"Role of hepatic carbonic anhydrase in de novo lipogenesis.","volume":"310","author":"Lynch","year":"1995","journal-title":"Biochem J"},{"key":"2021062512453770700_R28","doi-asserted-by":"crossref","first-page":"20551","DOI":"10.1074\/jbc.M003034200","article-title":"Bicarbonate binding activity of the CmpA protein of the cyanobacterium Synechococcus sp. strain PCC 7942 involved in active transport of bicarbonate.","volume":"275","author":"Maeda","year":"2000","journal-title":"J Biol Chem"},{"key":"2021062512453770700_R29","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1152\/physrev.1967.47.4.595","article-title":"Carbonic anhydrase: chemistry, physiology and inhibition.","volume":"47","author":"Maren","year":"1967","journal-title":"Physiol Rev"},{"key":"2021062512453770700_R30","doi-asserted-by":"crossref","first-page":"460","DOI":"10.1104\/pp.83.3.460","article-title":"Effects of carbonic anhydrase inhibitors on inorganic carbon accumulation by Chlamydomonas reinhardtii.","volume":"83","author":"Moroney","year":"1985","journal-title":"Plant Physiol"},{"key":"2021062512453770700_R31","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/S0378-1119(00)00411-X","article-title":"Evolutionary implications of the mosaic pyrimidine-biosynthetic pathway in eukaryotes.","volume":"257","author":"Nara","year":"2000","journal-title":"Gene"},{"key":"2021062512453770700_R32","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/0003-9861(84)90049-3","article-title":"Purification and characterization of maize leaf acetyl-coenzyme A carboxylase.","volume":"228","author":"Nikolau","year":"1984","journal-title":"Arch Biochem Biophys"},{"key":"2021062512453770700_R33","first-page":"957","article-title":"Lipid Biosynthesis.","volume":"7","author":"Ohlrogge","year":"1995","journal-title":"Plant Cell"},{"key":"2021062512453770700_R34","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1146\/annurev.arplant.48.1.109","article-title":"Regulation of fatty acid synthesis.","volume":"48","author":"Ohlrogge","year":"1997","journal-title":"Annu Rev Plant Physiol Plant Mol Biol"},{"key":"2021062512453770700_R35","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1016\/0005-2744(72)90255-0","article-title":"Intracellular distribution of carbonic anhydrase in spinach leaves.","volume":"258","author":"Poincelot","year":"1972","journal-title":"Biochim Biophys Acta"},{"key":"2021062512453770700_R36","doi-asserted-by":"crossref","first-page":"784","DOI":"10.1104\/pp.100.2.784","article-title":"Association of carbonic anhydrase activity with carboxysomes isolated from the cyanobacterium Synechococcus PCC 7942.","volume":"100","author":"Price","year":"1992","journal-title":"Plant Physiol"},{"key":"2021062512453770700_R37","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1007\/BF00201810","article-title":"Specific reduction of chloroplast carbonic anhydrase activity by antisense RNA in transgenic tobacco plants has a minor effect on photosynthetic CO2 assimilation.","volume":"193","author":"Price","year":"1994","journal-title":"Planta"},{"key":"2021062512453770700_R38","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1104\/pp.107.2.413","article-title":"The utilization of glycolytic intermediates as precursors for fatty acid biosynthesis by pea root plastids.","volume":"107","author":"Qi","year":"1995","journal-title":"Plant Physiol"},{"key":"2021062512453770700_R39","first-page":"47","article-title":"Carbonic anhydrase in plants: distribution, properties and possible physiological roles.","volume":"7","author":"Reed","year":"1981","journal-title":"Progress in Phytochemistry"},{"key":"2021062512453770700_R40","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1007\/BF00262637","article-title":"Co-purification, co-immunoprecipitation, and coordinate expression of acetyl-coenzyme A carboxylase activity, biotin carboxylase, and biotin carboxyl carrier protein of higher plants.","volume":"198","author":"Roesler","year":"1996","journal-title":"Planta"},{"key":"2021062512453770700_R41","doi-asserted-by":"crossref","first-page":"1239","DOI":"10.1104\/pp.110.4.1239","article-title":"Evidence that isolated chloroplasts contain an integrated lipid-synthesizing assembly that channels acetate into long-chain fatty acids.","volume":"110","author":"Roughan","year":"1996","journal-title":"Plant Physiol"},{"key":"2021062512453770700_R42","first-page":"83","article-title":"RNA blot Analysis.","volume-title":"A Laboratory Guide to RNA: Isolation, Analysis, and Synthesis.","author":"Sagerstro\u0308m","year":"1996"},{"key":"2021062512453770700_R43","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1146\/annurev.bi.64.070195.002111","article-title":"Human carbonic anhydrases and carbonic anhydrase deficiencies.","volume":"64","author":"Sly","year":"1995","journal-title":"Annu Rev Biochem"},{"key":"2021062512453770700_R44","doi-asserted-by":"crossref","first-page":"1260","DOI":"10.1104\/pp.70.5.1260","article-title":"Phosphatidylglycerol synthesis in spinach chloroplasts: characterization of the newly synthesized molecule.","volume":"70","author":"Sparace","year":"1982","journal-title":"Plant Physiol"},{"key":"2021062512453770700_R45","doi-asserted-by":"crossref","first-page":"602","DOI":"10.1104\/pp.96.2.602","article-title":"Characterization of fatty acid biosynthesis in isolated pea root plastids.","volume":"96","author":"Stahl","year":"1991","journal-title":"Plant Physiol"},{"key":"2021062512453770700_R46","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1007\/BF00224781","article-title":"Glycolytic enzymes in non-photosynthetic plastids of pea (Pisum sativum L.) roots.","volume":"190","author":"Trimming","year":"1993","journal-title":"Planta"},{"key":"2021062512453770700_R47","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1006\/abio.1994.1538","article-title":"A modified not borate method significantly enhances the yield of high-quality RNA from cotton (Gossypium hirsutum L.).","volume":"223","author":"Wan","year":"1994","journal-title":"Anal Biochem"},{"key":"2021062512453770700_R48","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/S0021-9258(18)51011-5","article-title":"Electrometric and colorimetric determination of carbonic anhydrase.","volume":"176","author":"Wilbur","year":"1948","journal-title":"J Biol Chem"},{"key":"2021062512453770700_R49","doi-asserted-by":"crossref","first-page":"1048","DOI":"10.1104\/pp.88.4.1048","article-title":"Glutamine transport and the role of the glutamine translocator in chloroplasts.","volume":"88","author":"Yu","year":"1988","journal-title":"Plant Physiol"}],"container-title":["Plant Physiology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/plphys\/article-pdf\/128\/4\/1417\/38687994\/plphys_v128_4_1417.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/plphys\/article-pdf\/128\/4\/1417\/38687994\/plphys_v128_4_1417.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,6,25]],"date-time":"2021-06-25T12:52:37Z","timestamp":1624625557000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/plphys\/article\/128\/4\/1417\/6110176"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,4,1]]},"references-count":49,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2002,4,1]]},"published-print":{"date-parts":[[2002,4,1]]}},"URL":"https:\/\/doi.org\/10.1104\/pp.010879","relation":{},"ISSN":["1532-2548","0032-0889"],"issn-type":[{"value":"1532-2548","type":"electronic"},{"value":"0032-0889","type":"print"}],"subject":[],"published-other":{"date-parts":[[2002,4,1]]},"published":{"date-parts":[[2002,4,1]]}}}