{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T02:48:26Z","timestamp":1767840506217,"version":"3.49.0"},"reference-count":47,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2002,12,1]],"date-time":"2002-12-01T00:00:00Z","timestamp":1038700800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2002,12,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Algae and vascular plants are cysteine (Cys) prototrophs. They are able to import, reduce, and assimilate sulfate into Cys, methionine, and other organic sulfur-containing compounds. Characterization of genes encoding the enzymes required for Cys biosynthesis from the unicellular green alga Chlamydomonas reinhardtii reveals that transcriptional and posttranscriptional mechanisms regulate the pathway. The derived amino acid sequences of the C. \u00a0reinhardtiigenes encoding 5\u2032-adenylylsulfate (APS) reductase and serine (Ser) acetyltransferase are orthologous to sequences from vascular plants. The Cys biosynthetic pathway of C. \u00a0reinhardtii is regulated by sulfate availability. The steady-state level of transcripts and activity of ATP sulfurylase, APS reductase, Ser acetyltransferase, and O-acetyl-Ser (thiol) lyase increase when cells are deprived of sulfate. Thesac1 mutation, which impairs C. \u00a0reinhardtii ability to acclimate to sulfur-deficient conditions, prevents the increase in accumulation of the transcripts encoding these enzymes and also prevents the increase in activity of all the enzymes except APS reductase. The sac2 mutation, which does not affect accumulation of APS reductase transcripts, blocks the increase in APS reductase activity. These results suggest that APS reductase activity is regulated posttranscriptionally in aSAC2-dependent process.<\/jats:p>","DOI":"10.1104\/pp.012484","type":"journal-article","created":{"date-parts":[[2002,12,12]],"date-time":"2002-12-12T18:22:30Z","timestamp":1039717350000},"page":"2076-2084","source":"Crossref","is-referenced-by-count":73,"title":["The <i>sac<\/i> Mutants of <i>Chlamydomonas reinhardtii<\/i> Reveal Transcriptional and Posttranscriptional Control of Cysteine Biosynthesis"],"prefix":"10.1093","volume":"130","author":[{"given":"Cristina G.","family":"Ravina","sequence":"first","affiliation":[{"name":"Instituto de Bioqu\u0131\u0301mica Vegetal y Fotos\u0131\u0301ntesis, Consejo Superior de Investigaciones Cientificas (C.G.R., C.G.) and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chwenn-In","family":"Chang","sequence":"additional","affiliation":[{"name":"Biotechnology Center for Agriculture and the Environment and the Department of Plant Biology and Pathology, Rutgers University, New Brunswick, New Jersey 08901\u20138520 (C.-I.C., G.P.T., T.L.); and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"George P.","family":"Tsakraklides","sequence":"additional","affiliation":[{"name":"Biotechnology Center for Agriculture and the Environment and the Department of Plant Biology and Pathology, Rutgers University, New Brunswick, New Jersey 08901\u20138520 (C.-I.C., G.P.T., T.L.); and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeffery P.","family":"McDermott","sequence":"additional","affiliation":[{"name":"Department of Botany, Iowa State University, Ames, Iowa 50011 (J.P.M., J.P.D.)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jose M.","family":"Vega","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u0131\u0301mica Vegetal y Biolog\u0131\u0301a Molecular, Facultad de Qu\u0131\u0301mica (J.M.V.), Universidad de Sevilla, 41092 Sevilla, 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