{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T01:54:12Z","timestamp":1767837252186,"version":"3.49.0"},"reference-count":28,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2003,9,1]],"date-time":"2003-09-01T00:00:00Z","timestamp":1062374400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2003,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>As an important step toward free access and, thus, impact of GoldenRice, a freedom-to-operate situation has been achieved for developing countries for the technology involved. Specifically, to carry the invention beyond its initial \u201cproof-of-concept\u201d status in a Japonica rice (Oryza sativa) cultivar, we report here on two transformed elite Indica varieties (IR64 and MTL250) plus one Japonica variety Taipei 309. Indica varieties are predominantly consumed in the areas with vitamin A deficiency. To conform with regulatory constraints, we changed the vector backbone, investigated the absence of beyond-border transfer, and relied on Agrobacterium tumefaciens-mediated transformation to obtain defined integration patterns. To avoid an antibiotic selection system, we now rely exclusively on phosphomannose isomerase as the selectable marker. Single integrations were given a preference to minimize potential epigenetic effects in subsequent generations. These novel lines, now in the T3 generation, are highly valuable because they are expected to more readily receive approval for follow-up studies such as nutritional and risk assessments and for breeding approaches leading to locally adapted variety development.<\/jats:p>","DOI":"10.1104\/pp.103.023457","type":"journal-article","created":{"date-parts":[[2003,9,12]],"date-time":"2003-09-12T01:05:44Z","timestamp":1063328744000},"page":"161-169","source":"Crossref","is-referenced-by-count":90,"title":["Golden Indica and Japonica Rice Lines Amenable to Deregulation"],"prefix":"10.1093","volume":"133","author":[{"given":"Tran Thi Cuc","family":"Hoa","sequence":"first","affiliation":[{"name":"Institute for Plant Sciences, Swiss Federal Institute of Technology, CH-8092 Zurich, Switzerland (T.T.C.H., I.P.); and University of Freiburg, Center for Applied Biosciences, Scha\u0308nzlestrasse 1, D-79104 Freiburg, Germany (S.A.-B., P.S., P.B.)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Salim","family":"Al-Babili","sequence":"additional","affiliation":[{"name":"Institute for Plant Sciences, Swiss Federal Institute of Technology, CH-8092 Zurich, Switzerland (T.T.C.H., I.P.); and University of Freiburg, Center for Applied Biosciences, Scha\u0308nzlestrasse 1, D-79104 Freiburg, Germany (S.A.-B., P.S., P.B.)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrick","family":"Schaub","sequence":"additional","affiliation":[{"name":"Institute for Plant Sciences, Swiss Federal Institute of Technology, CH-8092 Zurich, Switzerland (T.T.C.H., I.P.); and University of Freiburg, Center for Applied Biosciences, Scha\u0308nzlestrasse 1, D-79104 Freiburg, Germany (S.A.-B., P.S., P.B.)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ingo","family":"Potrykus","sequence":"additional","affiliation":[{"name":"Institute for Plant Sciences, Swiss Federal Institute of Technology, CH-8092 Zurich, Switzerland (T.T.C.H., I.P.); and University of Freiburg, Center for Applied Biosciences, Scha\u0308nzlestrasse 1, D-79104 Freiburg, Germany (S.A.-B., P.S., P.B.)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Beyer","sequence":"additional","affiliation":[{"name":"Institute for Plant Sciences, Swiss Federal Institute of Technology, CH-8092 Zurich, Switzerland (T.T.C.H., I.P.); and University of Freiburg, Center for Applied Biosciences, Scha\u0308nzlestrasse 1, D-79104 Freiburg, Germany (S.A.-B., P.S., P.B.)"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2003,9,11]]},"reference":[{"key":"2021062710530403900_REF1","unstructured":"Aldemita RR, Hodges TK (1996) Agrobacterium tumefaciens-mediated transformation of Japonica and Indica rice varieties. Planta \u00a0199 \u00a0: \u00a0612-617"},{"key":"2021062710530403900_REF2","doi-asserted-by":"crossref","unstructured":"Bartley GE, Scolnik PA, Beyer P (1999) Two Arabidopsis thaliana carotene desaturases, phytoene desaturase and \u03b6-carotene desaturase, expressed in Escherichia coli, catalyze a poly-cis pathway to yield prolycopene. Eur J Biochem \u00a0259 \u00a0: \u00a0396-403","DOI":"10.1046\/j.1432-1327.1999.00051.x"},{"key":"2021062710530403900_","unstructured":"Bosjen K, Donaldson I, Haldrup A, Joersboe M, Kreiberg JD, Nielsen J, Okkels FT, Peterson SG, Whenham RJ, inventors. November 30, 1999. Positive selection. United States Patent 5,994,629"},{"key":"2021062710530403900_","unstructured":"Bosjen K, Donaldson I, Haldrup A, Joersboe M, Kreiberg JD, Nielsen J, Okkels FT, Peterson SG, Whenham RJ, inventors. June 16, 1998. Mannose or xylose based positive selection. United States Patent 5,767,378"},{"key":"2021062710530403900_REF5","doi-asserted-by":"crossref","unstructured":"Burkhardt PK, Beyer P, Wu\u0308nn J, Kloti A, Armstrong GA, Schledz M, von Lintig V, Potrykus I (1997) Transgenic rice (Oryza sativa) endosperm expressing daffodil (Narcissus pseudonarcissus) phytoene synthase accumulates phytoene, a key intermediate of provitamin A biosynthesis. Plant J \u00a011 \u00a0: \u00a01071-1078","DOI":"10.1046\/j.1365-313X.1997.11051071.x"},{"key":"2021062710530403900_REF6","unstructured":"Gillespie S, Haddad L (2001) Attacking the double burden of malnutrition in Asia and the Pacific. ADB Nutrition and Development Series No. 4. http:\/\/www.adb.org\/Documents\/Books\/Nutrition\/Malnutrition\/default.asp"},{"key":"2021062710530403900_REF7","doi-asserted-by":"crossref","unstructured":"Hoa TTC, Bong BB, Huq E, Hodges TK (2002) Cre\/lox site-specific recombination controls the excision of the transgene from the rice genome. Theor Appl Genet \u00a0104 \u00a0: \u00a0518-525","DOI":"10.1007\/s001220100748"},{"key":"2021062710530403900_REF8","doi-asserted-by":"crossref","unstructured":"Hoekema A, Roelvink PW, Hooykaas PJJ, Schilperoort RA (1984) Delivery of T-DNA from the Agrobacterium tumefaciens chromosome into plant cells. EMBO J \u00a03 \u00a0: \u00a02485-2490","DOI":"10.1002\/j.1460-2075.1984.tb02160.x"},{"key":"2021062710530403900_REF9","doi-asserted-by":"crossref","unstructured":"Huang D, Ou B, Hampsch-Woodill M, Flanagan JA, Deemer EK (2002) Development and validation of oxygen radical absorbance capacity assay for lipophilicantioxidants using randomply methylated \u03b2-cyclodextrin as the solubility enhancer. Agric Food Chem \u00a050 \u00a0: \u00a01815-1821","DOI":"10.1021\/jf0113732"},{"key":"2021062710530403900_REF10","doi-asserted-by":"crossref","unstructured":"Isaacson T, Ronen G, Zamir D, Hirschberg J (2002) Cloning of tangerine from tomato reveals a carotenoid isomerase essential for the production of \u03b2-carotene and xanthophylls in plants. Plant Cell \u00a014 \u00a0: \u00a0332-343","DOI":"10.1105\/tpc.010303"},{"key":"2021062710530403900_REF11","doi-asserted-by":"crossref","unstructured":"Kononov ME, Bassuner B, Gelvin SB (1997) Integration of T-DNA binary vector \u201cbackbone\u201d sequences into the tobacco genome: evidence for multiple complex patterns of integration. Plant J \u00a011 \u00a0: \u00a0945-957","DOI":"10.1046\/j.1365-313X.1997.11050945.x"},{"key":"2021062710530403900_REF12","doi-asserted-by":"crossref","unstructured":"Lucca P, Ye X, Potrykus I (2001) Effective selection and regeneration of transgenic rice plants with mannose as selective agent. Mol Breed \u00a07 \u00a0: \u00a043-49","DOI":"10.1023\/A:1009661014167"},{"key":"2021062710530403900_REF13","doi-asserted-by":"crossref","unstructured":"McCouch SR, Kochehert G, Yu ZH, Wanh ZY, Khush GS, Coffman WR, Tanksley SD (1988) Molecular mapping of rice chromosomes. Theor Appl Genet \u00a078 \u00a0: \u00a0815-829","DOI":"10.1007\/BF00273666"},{"key":"2021062710530403900_REF14","doi-asserted-by":"crossref","unstructured":"Misawa N, Yamano S, Linden H, de Felipe MR, Lucas M, Ikenaga H, Sandmann G (1993) Functional expression of the Erwinia uredovora carotenoid biosynthesis gene crtl in transgenic plants showing an increase of beta-carotene biosynthesis activity and resistance to the bleaching herbicide norflurazon. Plant J \u00a04 \u00a0: \u00a0833-840","DOI":"10.1046\/j.1365-313X.1993.04050833.x"},{"key":"2021062710530403900_REF15","doi-asserted-by":"crossref","unstructured":"Murashige T, Skoog K (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant \u00a015 \u00a0: \u00a0473-497","DOI":"10.1111\/j.1399-3054.1962.tb08052.x"},{"key":"2021062710530403900_REF16","doi-asserted-by":"crossref","unstructured":"Park H, Kreunen SS, Cuttriss AJ, DellaPenna D, Pogson BJ (2002) Identification of the carotenoid isomerase provides insight into carotenoid biosynthesis, prolamellar body formation, and photomorphogenesis. Plant Cell \u00a014 \u00a0: \u00a0321-332","DOI":"10.1105\/tpc.010302"},{"key":"2021062710530403900_REF17","doi-asserted-by":"crossref","unstructured":"Redmond TM, Gentleman S, Duncan T, Yu S, Wiggert B, Gantt E, Cunningham FX Jr (2001) Identification, expression, and substrate specificity of a mammalian beta-carotene 15, 15\u2032-dioxygenase. J Biol Chem \u00a0276 \u00a0: \u00a06560-6565","DOI":"10.1074\/jbc.M009030200"},{"key":"2021062710530403900_REF18","unstructured":"Roberts C, Rajagopal S, Smith LM, Nguyen TA, Yang W, Nugrohu S, Ravi KS, Vijayachandra K, Harcourt RL, Dransfield L et al. (2000) A comprehensive set of modular vectors for advanced manipulations and efficient transformation of plant GenBank accession numbers: pCAMBIA 1200 (AF234292), 1380 (AF234301); 1390 (AF234307). http:\/\/www.cambia.org\/main\/r_et_vman.htm"},{"key":"2021062710530403900_REF19","doi-asserted-by":"crossref","unstructured":"Romer S, Fraser PD, Kiano JW, Shipton CA, Misawa N, Schuch W, Bramley PM (2000) Elevation of the provitamin A content of transgenic tomato plants. Nat Biotechnol \u00a018 \u00a0: \u00a0666-669","DOI":"10.1038\/76523"},{"key":"2021062710530403900_REF20","doi-asserted-by":"crossref","unstructured":"Underwood BA (2000) Overcoming micronutrient deficiencies in developing countries: Is there a role for agriculture? In N.S. Scimshaw, ed, Food and Nutrition Bulletin, Vol 21, No 4. United Nations University Press, Tokyo, Japan, pp 356-360. http:\/\/www.unu.edu\/unupress\/food\/fnb21-4.pdf","DOI":"10.1177\/156482650002100403"},{"key":"2021062710530403900_REF21","unstructured":"UNICEF (2000) The State of World's Children: Statistical Tables. UNICEF, New York"},{"key":"2021062710530403900_REF22","doi-asserted-by":"crossref","unstructured":"von Lintig J, Vogt K (2000) Filling the gap in vitamin A research: molecular identification of an enzyme cleaving beta-carotene to retinal. J Biol Chem \u00a0275 \u00a0: \u00a011915-11920","DOI":"10.1074\/jbc.275.16.11915"},{"key":"2021062710530403900_REF23","doi-asserted-by":"crossref","unstructured":"Xu Z, Godber JS (1999) Purification and identification of components of \u03b3-oryzanol in rice bran oil. J Agric Food Chem: 47 \u00a0: \u00a02724-2728","DOI":"10.1021\/jf981175j"},{"key":"2021062710530403900_REF24","unstructured":"Wu\u0308nn J, Klo\u0308ti A, Burhardt PK, Ghosh Biswas GC, Launis K, Iglesias VA, Potrykus I (1996) Transgenic indica rice breeding line IR58 expressing a synthetic cryIA(b) gene from Bacillus thuringiensis provides effective insect pest control. Bio\/Technology \u00a014 \u00a0: \u00a0171-176"},{"key":"2021062710530403900_REF25","doi-asserted-by":"crossref","unstructured":"Xu Z, Hua N, Godber JS (2001) antioxidant activity of tocopherols, tocotrienols, and \u03b3-oryzanol components from rice bran against cholesterol oxidation allererated by 2,2\u2032-azobis(2-methylpropronamidine)dihydrochloride. J Agric Food Chem: 49 \u00a0: \u00a02077-2081","DOI":"10.1021\/jf0012852"},{"key":"2021062710530403900_REF26","doi-asserted-by":"crossref","unstructured":"Yan W, Jang GF, Haeseleer F, Esumi N, Chang J, Kerrigan M, Campochiaro M, Campochiaro P, Palczewski K, Zack DJ (2001) Cloning and characterization of a human beta, beta-carotene-15,15\u2032-dioxygenase that is highly expressed in the retinal pigment epithelium. Genomics \u00a072 \u00a0: \u00a0193-202","DOI":"10.1006\/geno.2000.6476"},{"key":"2021062710530403900_REF27","doi-asserted-by":"crossref","unstructured":"Ye X, Al-Babili S, Klo\u0308ti A, Zhang J, Lucca P, Beyer P, Potrykus I (2000) Engineering the provitamin A (\u03b2-carotene) biosynthetic pathway into (carotenoid free) rice endosperm. Science \u00a0287 \u00a0: \u00a0303-305","DOI":"10.1126\/science.287.5451.303"},{"key":"2021062710530403900_REF28","unstructured":"Zimmermann R, Qaim M (2002) Projecting the benefits of Golden Rice in the Philippines. ZEF Discussion Papers on Development Policy No. 51, September 2002. ISSN: 1436-9931. Center for Development Research, Bonn. http:\/\/www.zef.de\/download\/zef_dp\/zef_dp51.PDF"}],"container-title":["Plant Physiology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/plphys\/article-pdf\/133\/1\/161\/38709089\/plphys_v133_1_161.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/plphys\/article-pdf\/133\/1\/161\/38709089\/plphys_v133_1_161.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,10]],"date-time":"2024-01-10T17:49:08Z","timestamp":1704908948000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/plphys\/article\/133\/1\/161\/6111859"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,9,1]]},"references-count":28,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2003,9,11]]},"published-print":{"date-parts":[[2003,9,1]]}},"URL":"https:\/\/doi.org\/10.1104\/pp.103.023457","relation":{},"ISSN":["1532-2548","0032-0889"],"issn-type":[{"value":"1532-2548","type":"electronic"},{"value":"0032-0889","type":"print"}],"subject":[],"published-other":{"date-parts":[[2003,9,1]]},"published":{"date-parts":[[2003,9,1]]}}}