{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T16:21:15Z","timestamp":1790871675972,"version":"4.1.0"},"reference-count":35,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2004,7,1]],"date-time":"2004-07-01T00:00:00Z","timestamp":1088640000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2004,7,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Pollen tube growth requires a Ca2+ gradient, with elevated levels of cytosolic Ca2+ at the growing tip. This gradient's magnitude oscillates with growth oscillation but is always maintained. Ca2+ influx into the growing tip is necessary, and its magnitude also oscillates with growth. It has been widely assumed that stretch-activated Ca2+ channels underlie this influx, but such channels have never been reported in either pollen grains or pollen tubes. We have identified and characterized stretch-activated Ca2+ channels from Lilium longiflorum pollen grain and tube tip protoplasts. The channels were localized to a small region of the grain protoplasts associated with the site of tube germination. In addition, we find a stretch-activated K+ channel as well as a spontaneous K+ channel distributed over the entire grain surface, but neither was present at the germination site or at the tip. Neither stretch-activated channel was detected in the grain protoplasts unless the grains were left in germination medium for at least 1 h before protoplast preparation. The stretch-activated channels were inhibited by a spider venom that is known to block stretch-activated channels in animal cells, but the spontaneous channel was unaffected by the venom. The venom also stopped pollen tube germination and elongation and blocked Ca2+ entry into the growing tip, suggesting that channel function is necessary for growth.<\/jats:p>","DOI":"10.1104\/pp.104.041483","type":"journal-article","created":{"date-parts":[[2004,7,20]],"date-time":"2004-07-20T01:52:54Z","timestamp":1090288374000},"page":"1398-1406","source":"Crossref","is-referenced-by-count":154,"title":["Identification and Characterization of Stretch-Activated Ion Channels in Pollen Protoplasts"],"prefix":"10.1093","volume":"135","author":[{"given":"Rajiv","family":"Dutta","sequence":"first","affiliation":[{"name":"Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenneth R.","family":"Robinson","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2004,7,19]]},"reference":[{"key":"2021062711170894400_R1","doi-asserted-by":"crossref","unstructured":"Brewbaker JL, Kwack BH (1963) The essential role of calcium ion in pollen germination and pollen tube growth. 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