{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T12:36:33Z","timestamp":1792499793934,"version":"4.5.0"},"reference-count":31,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2004,8,1]],"date-time":"2004-08-01T00:00:00Z","timestamp":1091318400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2004,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Indirect defense of plants against herbivores often involves the induced emission of volatile infochemicals including terpenoids that attract natural enemies of the herbivores. We report the isolation and characterization of a terpene synthase cDNA (LjE\u03b2OS) from a model legume, Lotus japonicus. Recombinant LjE\u03b2OS enzyme produced (E)-\u03b2-ocimene (98%) and its Z-isomer (2%). Transcripts of LjE\u03b2OS were induced in L. japonicus plants infested with two-spotted spider mites (Tetranychus urticae), coinciding with increasing emissions of (E)-\u03b2-ocimene as well as other volatiles, (Z)-3-hexenyl acetate and (E)-4,8-dimethyl-1,3,7-nonatriene, by the infested plants. We suggest that LjE\u03b2OS is involved in the herbivore-induced indirect defense response of spider mite-infested L. japonicus via de novo formation and emission (E)-\u03b2-ocimene. Mechanical wounding of the leaves or application of alamethicin (ALA), a potent fungal elicitor of plant volatile emission, also induced transiently increased levels of LjE\u03b2OS transcripts in L. japonicus. However, wounding or ALA did not result in elevated release of (E)-\u03b2-ocimene. Differences in volatile emissions after herbivory, mechanical wounding, or treatment with ALA suggest that neither a single mechanical wounding event nor ALA simulate the effect of herbivore activity and indicate that herbivore-induced emission of (E)-\u03b2-ocimene in L. japonicus involves control mechanisms in addition to up-regulation of LjE\u03b2OS transcripts.<\/jats:p>","DOI":"10.1104\/pp.104.042929","type":"journal-article","created":{"date-parts":[[2004,8,23]],"date-time":"2004-08-23T19:39:18Z","timestamp":1093289958000},"page":"1976-1983","source":"Crossref","is-referenced-by-count":157,"title":["Herbivore-Induced Defense Response in a Model Legume. Two-Spotted Spider Mites Induce Emission of (<i>E<\/i>)-<i>\u03b2<\/i>-Ocimene and Transcript Accumulation of (<i>E<\/i>)-<i>\u03b2<\/i>-Ocimene Synthase in <i>Lotus japonicus<\/i>\n\u00a0"],"prefix":"10.1093","volume":"135","author":[{"given":"Gen-ichiro","family":"Arimura","sequence":"first","affiliation":[{"name":"Biotechnology Laboratory, Department of Botany, and Department of Forest Sciences, University of British Columbia, Vancouver V6T 1Z3, British Columbia, Canada (G.A., J.B.); Center for Ecological Research, Kyoto University, Otsu 520\u20132113, Japan (R.O., S.K., J.T.); and CREST of Japan Science and Technology Agency, Kawaguchi 332\u20130012, Japan (R.O., J.T.)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rika","family":"Ozawa","sequence":"additional","affiliation":[{"name":"Biotechnology Laboratory, Department of Botany, and Department of Forest Sciences, University of British Columbia, Vancouver V6T 1Z3, British Columbia, Canada (G.A., J.B.); Center for Ecological Research, Kyoto University, Otsu 520\u20132113, Japan (R.O., S.K., J.T.); and CREST of Japan Science and Technology Agency, Kawaguchi 332\u20130012, Japan (R.O., J.T.)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soichi","family":"Kugimiya","sequence":"additional","affiliation":[{"name":"Biotechnology Laboratory, Department of Botany, and Department of Forest Sciences, University of British Columbia, Vancouver V6T 1Z3, British Columbia, Canada (G.A., J.B.); Center for Ecological Research, Kyoto University, Otsu 520\u20132113, Japan (R.O., S.K., J.T.); and CREST of Japan Science and Technology Agency, Kawaguchi 332\u20130012, Japan (R.O., J.T.)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junji","family":"Takabayashi","sequence":"additional","affiliation":[{"name":"Biotechnology Laboratory, Department of Botany, and Department of Forest Sciences, University of British Columbia, Vancouver V6T 1Z3, British Columbia, Canada (G.A., J.B.); Center for Ecological Research, Kyoto University, Otsu 520\u20132113, Japan (R.O., S.K., J.T.); and CREST of Japan Science and Technology Agency, Kawaguchi 332\u20130012, Japan (R.O., J.T.)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u0308rg","family":"Bohlmann","sequence":"additional","affiliation":[{"name":"Biotechnology Laboratory, Department of Botany, and Department of Forest Sciences, University of British Columbia, Vancouver V6T 1Z3, British Columbia, Canada (G.A., J.B.); Center for Ecological Research, Kyoto University, Otsu 520\u20132113, Japan (R.O., S.K., J.T.); and CREST of Japan Science and Technology Agency, Kawaguchi 332\u20130012, Japan (R.O., J.T.)"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2004,8,23]]},"reference":[{"key":"2021062605241571600_R1","doi-asserted-by":"crossref","unstructured":"Aharoni A, Giri AP, Deuerlein S, Griepink F, de Kogel WJ, Verstappen FW, Verhoeven HA, Jongsma MA, Schwab W, Bouwmeester HJ (2003) Terpenoid metabolism in wild-type and transgenic Arabidopsis plants. Plant Cell \u00a015\n\u00a0: \u00a02866\u20132884","DOI":"10.1105\/tpc.016253"},{"key":"2021062605241571600_R2","doi-asserted-by":"crossref","unstructured":"Akamine S, Nakamori K, Chechetka SA, Banba M, Umehara Y, Kouchi H, Izui K, Hata S (2003) cDNA cloning, mRNA expression, and mutational analysis of the squalene synthase gene of Lotus japonicus. \u00a0Biochim Biophys Acta \u00a01626\n\u00a0: \u00a097\u2013101","DOI":"10.1016\/S0167-4781(03)00042-3"},{"key":"2021062605241571600_R3","doi-asserted-by":"crossref","unstructured":"Arimura G, Huber DPW, Bohlmann J (2004) Forest tent caterpillars (Malacosoma disstria) induce local and systemic diurnal emissions of terpenoid volatiles in hybrid poplar (Populus trichocarpa x deltoides): cDNA cloning, functional characterization, and patterns of gene expression of (\u2212)-germacrene D synthase, PtdTPS1. \u00a0Plant J \u00a037\n\u00a0: \u00a0603\u2013616","DOI":"10.1111\/j.1365-313X.2003.01987.x"},{"key":"2021062605241571600_R4","doi-asserted-by":"crossref","unstructured":"Arimura G, Ozawa R, Nishioka T, Boland W, Koch T, Ku\u0308hnemann F, Takabayashi J (2002) Herbivore-induced volatiles induce the emission of ethylene in neighboring lima bean plants. 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