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The hsi2 mutant contained a nonsense mutation in a gene encoding a protein with B3 DNA-binding domain. HSI2 and two other Arabidopsis proteins appear to constitute a novel subfamily of B3 domain proteins distinct from ABI3, FUS3, and LEC2, which are transcription activators involved in seed development. The C-terminal part of HSI2 subfamily proteins contained a sequence similar to the ERF-associated amphiphilic repression (EAR) motif. Deletion of the C-terminal portion of HSI2 lost in the hsi2 mutant caused reduced nuclear targeting of HSI2. Null allele of HSI2 showed even higher Spomin\u2237LUC expression than the hsi2 mutant, whereas overexpression of HSI2 reduced the LUC expression. Transient coexpression of 35S\u2237HSI2 with Spomin\u2237LUC in protoplasts repressed the expression of LUC activity, and deletion or mutation of the EAR motif significantly reduced the repression activity of HSI2. These results indicate that HSI2 and related proteins are B3 domain-EAR motif active transcription repressors.<\/jats:p>","DOI":"10.1104\/pp.104.057752","type":"journal-article","created":{"date-parts":[[2005,5,14]],"date-time":"2005-05-14T00:33:53Z","timestamp":1116030833000},"page":"675-685","source":"Crossref","is-referenced-by-count":106,"title":["Analysis of a Sugar Response Mutant of Arabidopsis Identified a Novel B3 Domain Protein That Functions as an Active Transcriptional Repressor"],"prefix":"10.1093","volume":"138","author":[{"given":"Hironaka","family":"Tsukagoshi","sequence":"first","affiliation":[{"name":"Laboratory of Biochemistry, Graduate School of Bioagricultural Science, Nagoya University, Chikusa, Nagoya 464\u20138601, Japan (H.T., T.S., K.N.); Kazusa DNA Research Institute, Kisarazu, Chiba 292\u20130818, Japan (D.S.); and College of Bioscience and Biotechnology, Chubu University, Kasugai, Aichi 487\u20138501, Japan (A.M.)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takanori","family":"Saijo","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemistry, Graduate School of Bioagricultural Science, Nagoya University, Chikusa, Nagoya 464\u20138601, Japan (H.T., T.S., K.N.); Kazusa DNA Research Institute, Kisarazu, Chiba 292\u20130818, Japan (D.S.); and College of Bioscience and Biotechnology, Chubu University, Kasugai, Aichi 487\u20138501, Japan (A.M.)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daisuke","family":"Shibata","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemistry, Graduate School of Bioagricultural Science, Nagoya University, Chikusa, Nagoya 464\u20138601, Japan (H.T., T.S., K.N.); Kazusa DNA Research Institute, Kisarazu, Chiba 292\u20130818, Japan (D.S.); and College of Bioscience and Biotechnology, Chubu University, Kasugai, Aichi 487\u20138501, Japan (A.M.)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atsushi","family":"Morikami","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemistry, Graduate School of Bioagricultural Science, Nagoya University, Chikusa, Nagoya 464\u20138601, Japan (H.T., T.S., K.N.); Kazusa DNA Research Institute, Kisarazu, Chiba 292\u20130818, Japan (D.S.); and College of Bioscience and Biotechnology, Chubu University, Kasugai, Aichi 487\u20138501, Japan (A.M.)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenzo","family":"Nakamura","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemistry, Graduate School of Bioagricultural Science, Nagoya University, Chikusa, Nagoya 464\u20138601, Japan (H.T., T.S., K.N.); Kazusa DNA Research Institute, Kisarazu, Chiba 292\u20130818, Japan (D.S.); and College of Bioscience and Biotechnology, Chubu University, Kasugai, Aichi 487\u20138501, Japan (A.M.)"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2005,5,13]]},"reference":[{"key":"2021050610580838800_R1","doi-asserted-by":"crossref","unstructured":"Arenas-Huertero F, Arroyo A, Zhou L, Sheen J, Leon P (2000) Analysis of Arabidopsis glucose insensitive mutants, gin5 and gin6, reveals a central role of the plant hormone ABA in the regulation of plant vegetative development by sugar. 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