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As a result of the rapid sampling, extraction, and analysis by gas chromatography coupled to time-of-flight mass spectrometry, more than 800 analytes from a single sample could be measured, of which more than 100 could be identified. Analyte responses could be determined mostly with ses less than 10%. Wild-type cells of C. reinhardtii strain CC-125 subjected to nitrogen-, phosphorus-, sulfur-, or iron-depleted growth conditions develop highly distinctive metabolite profiles. Individual metabolites undergo marked changes in their steady-state levels. Compared to control conditions, sulfur-depleted cells accumulated 4-hydroxyproline more than 50-fold, whereas the amount of 2-ketovaline was reduced to 2% of control levels. The contribution of each compound to the differences observed in the metabolic phenotypes is summarized in a quantitatively rigorous way by principal component analysis, which clearly discriminates the cells from different growth regimes and indicates that phosphorus-depleted conditions induce a deficiency syndrome quite different from the response to nitrogen, sulfur, or iron starvation.<\/jats:p>","DOI":"10.1104\/pp.105.071589","type":"journal-article","created":{"date-parts":[[2005,11,24]],"date-time":"2005-11-24T01:33:22Z","timestamp":1132796002000},"page":"1995-2005","source":"Crossref","is-referenced-by-count":194,"title":["Metabolite Profiling of <i>Chlamydomonas reinhardtii<\/i> under Nutrient Deprivation"],"prefix":"10.1093","volume":"139","author":[{"given":"Christian","family":"Bo\u0308lling","sequence":"first","affiliation":[{"name":"Max Planck Institute of Molecular Plant Physiology, 14424 Potsdam, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oliver","family":"Fiehn","sequence":"additional","affiliation":[{"name":"Max Planck Institute of Molecular Plant Physiology, 14424 Potsdam, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2005,11,25]]},"reference":[{"key":"2021050617163502600_R1","doi-asserted-by":"crossref","unstructured":"Abadia J, Lopez-Millan AF, Rombola A, Abadia A (2002) Organic acids and Fe deficiency: a review. 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