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Confocal imaging of intracellular Zn using Zinquin showed that Zn was preferentially accumulated in trichomes. Exposure to Zn and Zn + Ca increased the trichome density and induced the production of Ca\/Zn mineral grains on the head cells of trichomes. These grains were aggregates of submicrometer-sized crystals and poorly crystalline material and contained Ca as major element, along with subordinate amounts of Zn, manganese, potassium, chlorine, phosphorus, silicon, and magnesium. Micro x-ray diffraction revealed that the large majority of the grains were composed essentially of metal-substituted calcite (CaCO3). CaCO3 polymorphs (aragonite and vaterite) and CaC2O4 (Ca oxalate) mono- and dihydrate also were identified, either as an admixture to calcite or in separate grains. Some grains did not diffract, although they contained Ca, suggesting the presence of amorphous form of Ca. The presence of Zn-substituted calcite was confirmed by Zn K-edge micro-extended x-ray absorption fine structure spectroscopy. Zn bound to organic compounds and Zn-containing silica and phosphate were also identified by this technique. The proportion of Zn-substituted calcite relative to the other species increased with Ca exposure. The production of Zn-containing biogenic calcite and other Zn compounds through the trichomes is a novel mechanism involved in Zn detoxification. This study illustrates the potential of laterally resolved x-ray synchrotron radiation techniques to study biomineralization and metal homeostasis processes in plants.<\/jats:p>","DOI":"10.1104\/pp.106.082743","type":"journal-article","created":{"date-parts":[[2006,5,27]],"date-time":"2006-05-27T00:45:32Z","timestamp":1148690732000},"page":"1021-1034","source":"Crossref","is-referenced-by-count":139,"title":["Trichomes of Tobacco Excrete Zinc as Zinc-Substituted Calcium Carbonate and Other Zinc-Containing Compounds"],"prefix":"10.1093","volume":"141","author":[{"given":"Ge\u0301raldine","family":"Sarret","sequence":"first","affiliation":[{"name":"Environmental Geochemistry Group, Laboratoire de Ge\u0301ophysique Interne et Tectonophysique, University of Grenoble and Centre National de la Recherche Scientifique, 38041 Grenoble cedex 9, France (G.S., E.H., M.-P.I., N.G., A.M.); Division of Forest Resources, College of Forest Sciences, Kangwon National University, Chunchon 200\u2013701, Kangwon-do, Korea (E.H., Y.-E.C.); 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