{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T10:10:20Z","timestamp":1791281420847,"version":"4.1.0"},"reference-count":63,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2021,1,25]],"date-time":"2021-01-25T00:00:00Z","timestamp":1611532800000},"content-version":"vor","delay-in-days":4910,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2007,10,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Salicylic acid (SA) plays a central role in plant disease resistance, and emerging evidence indicates that auxin, an essential plant hormone in regulating plant growth and development, is involved in plant disease susceptibility. GH3.5, a member of the GH3 family of early auxin-responsive genes in Arabidopsis (Arabidopsis thaliana), encodes a protein possessing in vitro adenylation activity on both indole-3-acetic acid (IAA) and SA. Here, we show that GH3.5 acts as a bifunctional modulator in both SA and auxin signaling during pathogen infection. Overexpression of the GH3.5 gene in an activation-tagged mutant gh3.5-1D led to elevated accumulation of SA and increased expression of PR-1 in local and systemic tissues in response to avirulent pathogens. In contrast, two T-DNA insertional mutations of GH3.5 partially compromised the systemic acquired resistance associated with diminished PR-1 expression in systemic tissues. The gh3.5-1D mutant also accumulated high levels of free IAA after pathogen infection and impaired different resistance-gene-mediated resistance, which was also observed in the GH3.6 activation-tagged mutant dfl1-D that impacted the auxin pathway, indicating an important role of GH3.5\/GH3.6 in disease susceptibility. Furthermore, microarray analysis showed that the SA and auxin pathways were simultaneously augmented in gh3.5-1D after infection with an avirulent pathogen. The SA pathway was amplified by GH3.5 through inducing SA-responsive genes and basal defense components, whereas the auxin pathway was derepressed through up-regulating IAA biosynthesis and down-regulating auxin repressor genes. Taken together, our data reveal novel regulatory functions of GH3.5 in the plant-pathogen interaction.<\/jats:p>","DOI":"10.1104\/pp.107.106021","type":"journal-article","created":{"date-parts":[[2007,8,18]],"date-time":"2007-08-18T00:53:59Z","timestamp":1187398439000},"page":"450-464","source":"Crossref","is-referenced-by-count":291,"title":["Dual Regulation Role of <i>GH3.5<\/i> in Salicylic Acid and Auxin Signaling during Arabidopsis-<i>Pseudomonas syringae<\/i> Interaction"],"prefix":"10.1093","volume":"145","author":[{"given":"Zhongqin","family":"Zhang","sequence":"first","affiliation":[{"name":"National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China (Z.Z., Q.L., M.W., Y.Z., Z.H.); Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China (Z.L.); and Department of Agronomy and Horticulture, University of Nebraska, Lincol"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qun","family":"Li","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China (Z.Z., Q.L., M.W., Y.Z., Z.H.); Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China (Z.L.); and Department of Agronomy and Horticulture, University of Nebraska, Lincol"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhimiao","family":"Li","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China (Z.Z., Q.L., M.W., Y.Z., Z.H.); Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China (Z.L.); and Department of Agronomy and Horticulture, University of Nebraska, Lincol"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul E.","family":"Staswick","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China (Z.Z., Q.L., M.W., Y.Z., Z.H.); Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China (Z.L.); and Department of Agronomy and Horticulture, University of Nebraska, Lincol"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muyang","family":"Wang","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China (Z.Z., Q.L., M.W., Y.Z., Z.H.); Institute of 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