{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T21:22:04Z","timestamp":1791408124625,"version":"4.3.3"},"reference-count":43,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2021,1,25]],"date-time":"2021-01-25T00:00:00Z","timestamp":1611532800000},"content-version":"vor","delay-in-days":3940,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,6,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Phosphorus (P) enters roots as inorganic phosphate (Pi) derived from organic and inorganic P compounds in the soil. Nucleic acids can support plant growth as the sole source of P in axenic culture but are thought to be converted into Pi by plant-derived nucleases and phosphatases prior to uptake. Here, we show that a nuclease-resistant analog of DNA is taken up by plant cells. Fluorescently labeled S-DNA of 25 bp, which is protected against enzymatic breakdown by its phosphorothioate backbone, was taken up and detected in root cells including root hairs and pollen tubes. These results indicate that current views of plant P acquisition may have to be revised to include uptake of DNA into cells. We further show that addition of DNA to Pi-containing growth medium enhanced the growth of lateral roots and root hairs even though plants were P replete and had similar biomass as plants supplied with Pi only. Exogenously supplied DNA increased length growth of pollen tubes, which were studied because they have similar elongated and polarized growth as root hairs. Our results indicate that DNA is not only taken up and used as a P source by plants, but ironically and independent of Pi supply, DNA also induces morphological changes in roots similar to those observed with P limitation. This study provides, to our knowledge, first evidence that exogenous DNA could act nonspecifically as signaling molecules for root development.<\/jats:p>","DOI":"10.1104\/pp.110.154963","type":"journal-article","created":{"date-parts":[[2010,4,14]],"date-time":"2010-04-14T00:49:39Z","timestamp":1271206179000},"page":"799-805","source":"Crossref","is-referenced-by-count":72,"title":["DNA Is Taken Up by Root Hairs and Pollen, and Stimulates Root and Pollen Tube Growth\n\u00a0\n\u00a0"],"prefix":"10.1093","volume":"153","author":[{"given":"Chanyarat","family":"Paungfoo-Lonhienne","sequence":"first","affiliation":[{"name":"School of Biological Sciences (C.P.-L., S.R.M., P.M.S., S.S.) and the Australian Research Council Centre of Excellence for Integrative Legume Research and School of Chemistry and Molecular Biosciences (T.G.A.L., M.C., B.J.C.), University of Queensland, St. Lucia, Queensland 4072, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thierry G.A.","family":"Lonhienne","sequence":"additional","affiliation":[{"name":"School of Biological Sciences (C.P.-L., S.R.M., P.M.S., S.S.) and the Australian Research Council Centre of Excellence for Integrative Legume Research and School of Chemistry and Molecular Biosciences (T.G.A.L., M.C., B.J.C.), University of Queensland, St. Lucia, Queensland 4072, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen R.","family":"Mudge","sequence":"additional","affiliation":[{"name":"School of Biological Sciences (C.P.-L., S.R.M., P.M.S., S.S.) and the Australian Research Council Centre of Excellence for Integrative Legume Research and School of Chemistry and Molecular Biosciences (T.G.A.L., M.C., B.J.C.), University of Queensland, St. Lucia, Queensland 4072, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peer M.","family":"Schenk","sequence":"additional","affiliation":[{"name":"School of Biological Sciences (C.P.-L., S.R.M., P.M.S., S.S.) and the Australian Research Council Centre of Excellence for Integrative Legume Research and School of Chemistry and Molecular Biosciences (T.G.A.L., M.C., B.J.C.), University of Queensland, St. Lucia, Queensland 4072, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Christie","sequence":"additional","affiliation":[{"name":"School of Biological Sciences (C.P.-L., S.R.M., P.M.S., S.S.) and the Australian Research Council Centre of Excellence for Integrative Legume Research and School of Chemistry and Molecular Biosciences (T.G.A.L., M.C., B.J.C.), University of Queensland, St. Lucia, Queensland 4072, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernard J.","family":"Carroll","sequence":"additional","affiliation":[{"name":"School of Biological Sciences (C.P.-L., S.R.M., P.M.S., S.S.) and the Australian Research Council Centre of Excellence for Integrative Legume Research and School of Chemistry and Molecular Biosciences (T.G.A.L., M.C., B.J.C.), University of Queensland, St. Lucia, Queensland 4072, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susanne","family":"Schmidt","sequence":"additional","affiliation":[{"name":"School of Biological Sciences (C.P.-L., S.R.M., P.M.S., S.S.) and the Australian Research Council Centre of Excellence for Integrative Legume Research and School of Chemistry and Molecular Biosciences (T.G.A.L., M.C., B.J.C.), University of Queensland, St. Lucia, Queensland 4072, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2010,4,13]]},"reference":[{"key":"2021041717033388100_b1","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1104\/pp.122.2.543","article-title":"Induction of an extracellular cyclic nucleotide phosphodiesterase as an accessory ribonucleolytic activity during phosphate starvation of cultured tomato cells","volume":"122","author":"Abel","year":"2000","journal-title":"Plant Physiol"},{"key":"2021041717033388100_b2","first-page":"411","article-title":"Assessing organic phosphorus in soils","author":"Anderson","year":"1980"},{"key":"2021041717033388100_b3","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1111\/j.1365-3040.1996.tb00386.x","article-title":"Stimulation of root hair elongation in Arabidopsis thaliana by low phosphorus availability","volume":"19","author":"Bates","year":"1996","journal-title":"Plant Cell Environ"},{"key":"2021041717033388100_b4","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1146\/annurev.pp.24.060173.001301","article-title":"Phosphate pools, phosphate transport, and phosphate availability","volume":"24","author":"Bieleski","year":"1973","journal-title":"Annu Rev Plant Physiol"},{"key":"2021041717033388100_b5","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1097\/00010694-194504000-00003","article-title":"Separation and 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