{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T06:05:31Z","timestamp":1790316331599,"version":"4.1.0"},"reference-count":43,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2021,1,25]],"date-time":"2021-01-25T00:00:00Z","timestamp":1611532800000},"content-version":"vor","delay-in-days":3428,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2011,11,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Systemic acquired resistance (SAR) is a defense mechanism induced in the distal parts of plants after primary infection. It confers long-lasting protection against a broad spectrum of microbial pathogens. Lack of high-throughput assays has hampered the forward genetic analysis of SAR. Here, we report the development of an easy and efficient assay for SAR and its application in a forward genetic screen for SAR-deficient mutants in Arabidopsis (Arabidopsis thaliana). Using the new assay for SAR, we identified six flavin-dependent monooxygenase1, four AGD2-like defense response protein1, three salicylic acid induction-deficient2, one phytoalexin deficient4, and one avrPphB-susceptible3 alleles as well as a gain-of-function mutant of CALMODULIN-BINDING TRANSCRIPTION ACTIVATOR3 designated camta3-3D. Like transgenic plants overexpressing CAMTA3, camta3-3D mutant plants exhibit compromised SAR and enhanced susceptibility to virulent pathogens, suggesting that CAMTA3 is a critical regulator of both basal resistance and SAR.<\/jats:p>","DOI":"10.1104\/pp.111.182089","type":"journal-article","created":{"date-parts":[[2011,9,7]],"date-time":"2011-09-07T20:15:36Z","timestamp":1315426536000},"page":"973-980","source":"Crossref","is-referenced-by-count":62,"title":["Brush and Spray: A High-Throughput Systemic Acquired Resistance Assay Suitable for Large-Scale Genetic Screening\n\u00a0\n\u00a0"],"prefix":"10.1093","volume":"157","author":[{"given":"Beibei","family":"Jing","sequence":"first","affiliation":[{"name":"College of Biological Sciences, China Agricultural University, Beijing 100094, People\u2019s Republic of China (B.J.); National Institute of Biological Sciences, Beijing 102206, People\u2019s Republic of China (B.J., S.X., M.X., Y.L., S.L., J.D., Y.Z.)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaohua","family":"Xu","sequence":"additional","affiliation":[{"name":"College of Biological Sciences, China Agricultural University, Beijing 100094, People\u2019s Republic of China (B.J.); National Institute of Biological Sciences, Beijing 102206, People\u2019s Republic of China (B.J., S.X., M.X., Y.L., S.L., J.D., Y.Z.)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mo","family":"Xu","sequence":"additional","affiliation":[{"name":"College of Biological Sciences, China Agricultural University, Beijing 100094, People\u2019s Republic of China (B.J.); National Institute of Biological Sciences, Beijing 102206, People\u2019s Republic of China (B.J., S.X., M.X., Y.L., S.L., J.D., Y.Z.)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Li","sequence":"additional","affiliation":[{"name":"College of Biological Sciences, China Agricultural University, Beijing 100094, People\u2019s Republic of China (B.J.); National Institute of Biological Sciences, Beijing 102206, People\u2019s Republic of China (B.J., S.X., M.X., Y.L., S.L., J.D., Y.Z.)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuxin","family":"Li","sequence":"additional","affiliation":[{"name":"College of Biological Sciences, China Agricultural University, Beijing 100094, People\u2019s Republic of China (B.J.); National Institute of Biological Sciences, Beijing 102206, People\u2019s Republic of China (B.J., S.X., M.X., Y.L., S.L., J.D., Y.Z.)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinmei","family":"Ding","sequence":"additional","affiliation":[{"name":"College of Biological Sciences, China Agricultural University, Beijing 100094, People\u2019s Republic of China (B.J.); National Institute of Biological Sciences, Beijing 102206, People\u2019s Republic of China (B.J., S.X., M.X., Y.L., S.L., J.D., Y.Z.)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuelin","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Biological Sciences, China Agricultural University, Beijing 100094, People\u2019s Republic of China (B.J.); National Institute of Biological Sciences, Beijing 102206, People\u2019s Republic of China (B.J., S.X., M.X., Y.L., S.L., J.D., Y.Z.)"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2011,9,7]]},"reference":[{"key":"2021041908450593200_b1","doi-asserted-by":"crossref","first-page":"2148","DOI":"10.1126\/science.284.5423.2148","article-title":"EIN2, a bifunctional transducer of ethylene and stress responses in Arabidopsis","volume":"284","author":"Alonso","year":"1999","journal-title":"Science"},{"key":"2021041908450593200_b2","doi-asserted-by":"crossref","first-page":"1038","DOI":"10.1105\/tpc.105.039982","article-title":"Salicylic acid-independent ENHANCED DISEASE 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