{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T21:36:35Z","timestamp":1791236195219,"version":"4.1.0"},"reference-count":32,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2012,10,22]],"date-time":"2012-10-22T00:00:00Z","timestamp":1350864000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012,12,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Phytic acid (inositol hexakisphosphate [InsP6]) is the storage compound of phosphorus in seeds. As phytic acid binds strongly to metallic cations, it also acts as a storage compound of metals. To understand the mechanisms underlying metal accumulation and localization in relation to phytic acid storage, we applied synchrotron-based x-ray microfluorescence imaging analysis to characterize the simultaneous subcellular distribution of some mineral elements (phosphorus, calcium, potassium, iron, zinc, and copper) in immature and mature rice (Oryza sativa) seeds. This fine-imaging method can reveal whether these elements colocalize. We also determined their accumulation patterns and the changes in phosphate and InsP6 contents during seed development. While the InsP6 content in the outer parts of seeds rapidly increased during seed development, the phosphate contents of both the outer and inner parts of seeds remained low. Phosphorus, calcium, potassium, and iron were most abundant in the aleurone layer, and they colocalized throughout seed development. Zinc was broadly distributed from the aleurone layer to the inner endosperm. Copper localized outside the aleurone layer and did not colocalize with phosphorus. From these results, we suggest that phosphorus translocated from source organs was immediately converted to InsP6 and accumulated in aleurone layer cells and that calcium, potassium, and iron accumulated as phytic acid salt (phytate) in the aleurone layer, whereas zinc bound loosely to InsP6 and accumulated not only in phytate but also in another storage form. Copper accumulated in the endosperm and may exhibit a storage form other than phytate.<\/jats:p>","DOI":"10.1104\/pp.112.206573","type":"journal-article","created":{"date-parts":[[2012,10,24]],"date-time":"2012-10-24T00:19:18Z","timestamp":1351037958000},"page":"2007-2014","source":"Crossref","is-referenced-by-count":154,"title":["Dynamic Changes in the Distribution of Minerals in Relation to Phytic Acid Accumulation during Rice Seed Development\n\u00a0"],"prefix":"10.1093","volume":"160","author":[{"given":"Toru","family":"Iwai","sequence":"first","affiliation":[{"name":"Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113\u20138657, Japan (T.I., K.O., K.T.Y.); Faculty of Agriculture, Utsunomiya University, Utsunomiya, Tochigi 321\u20138505, Japan (M.K.); and Super Photon Ring-8, Japan Synchrotron Radiation Research Institute, Sayo-cho, Sayo-gun, Hyogo 679\u20135198, Japan (Y.T.)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michiko","family":"Takahashi","sequence":"additional","affiliation":[{"name":"Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113\u20138657, Japan (T.I., K.O., K.T.Y.); Faculty of Agriculture, Utsunomiya University, Utsunomiya, Tochigi 321\u20138505, Japan (M.K.); and Super Photon Ring-8, Japan Synchrotron Radiation Research Institute, Sayo-cho, Sayo-gun, Hyogo 679\u20135198, Japan (Y.T.)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koshiro","family":"Oda","sequence":"additional","affiliation":[{"name":"Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113\u20138657, Japan (T.I., K.O., K.T.Y.); Faculty of Agriculture, Utsunomiya University, Utsunomiya, Tochigi 321\u20138505, Japan (M.K.); and Super Photon Ring-8, Japan Synchrotron Radiation Research Institute, Sayo-cho, Sayo-gun, Hyogo 679\u20135198, Japan (Y.T.)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuko","family":"Terada","sequence":"additional","affiliation":[{"name":"Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113\u20138657, Japan (T.I., K.O., K.T.Y.); Faculty of Agriculture, Utsunomiya University, Utsunomiya, Tochigi 321\u20138505, Japan (M.K.); and Super Photon Ring-8, Japan Synchrotron Radiation Research Institute, Sayo-cho, Sayo-gun, Hyogo 679\u20135198, Japan (Y.T.)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaoru T.","family":"Yoshida","sequence":"additional","affiliation":[{"name":"Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113\u20138657, Japan (T.I., K.O., K.T.Y.); Faculty of Agriculture, Utsunomiya University, Utsunomiya, Tochigi 321\u20138505, Japan (M.K.); and Super Photon Ring-8, Japan Synchrotron Radiation Research Institute, Sayo-cho, Sayo-gun, Hyogo 679\u20135198, Japan (Y.T.)"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2012,10,22]]},"reference":[{"key":"2021052607362657900_b1","doi-asserted-by":"crossref","first-page":"7547","DOI":"10.1021\/jf071191t","article-title":"Quantitative analysis of phytate globoids isolated from wheat bran and characterization of their sequential dephosphorylation by wheat phytase","volume":"55","author":"Bohn","year":"2007","journal-title":"J Agric Food Chem"},{"key":"2021052607362657900_b2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1093\/aob\/mcn207","article-title":"Metal movement within the plant: contribution of nicotianamine and yellow stripe 1-like transporters","volume":"103","author":"Curie","year":"2009","journal-title":"Ann Bot (Lond)"},{"key":"2021052607362657900_b3","author":"Epstein"},{"key":"2021052607362657900_b4","doi-asserted-by":"crossref","first-page":"1068","DOI":"10.1039\/b803602k","article-title":"Micro x-ray fluorescence 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