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Ascorbate peroxidase, glutathione reductase, and monodehydroascorbate reductase activities were similar in the leaves from all of the lines, but dehydroascorbate reductase activity was increased in the leaves of the FeSOD transformants relative to untransformed controls. Foliar H2O2, ascorbate, and glutathione contents were comparable in all lines of plants. Irradiance-dependent changes in net CO2 assimilation and chlorophyll a fluorescence quenching parameters were similar in all lines both in air (21% O2) and at low (1%) O2. CO2-response curves for photosynthesis showed similar net CO2-exchange characteristics in all lines. In contrast, values of photochemical quenching declined in leaves from untransformed controls at intercellular CO2 (Ci) values below 200 \u03bcL L\u22121 but remained constant with decreasing Ci in leaves of FeSOD transformants. When the O2 concentration was decreased from 21 to 1%, the effect of FeSOD overexpression on photochemical quenching at limiting Ci was abolished. At high light (1000 \u03bcmol m\u22122s\u22121) a progressive decrease in the ratio of variable (Fv) to maximal (Fm) fluorescence was observed with decreasing temperature. At 6oC the high-light-induced decrease in theFv\/Fm ratio was partially prevented by low O2 but values were comparable in all lines. Methyl viologen caused decreasedFv\/Fm ratios, but this was less marked in the FeSOD transformants than in the untransformed controls. These observations suggest that the rate of superoxide dismutation limits flux through the Mehler-peroxidase cycle in certain conditions.<\/jats:p>","DOI":"10.1104\/pp.117.2.565","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T05:00:20Z","timestamp":1027746020000},"page":"565-574","source":"Crossref","is-referenced-by-count":79,"title":["Overexpression of Iron Superoxide Dismutase in Transformed Poplar Modifies the Regulation of Photosynthesis at Low CO2Partial Pressures or Following Exposure to the Prooxidant Herbicide Methyl Viologen1"],"prefix":"10.1093","volume":"117","author":[{"given":"Ana-Carolina M.","family":"Arisi","sequence":"first","affiliation":[{"name":"Laboratoire de Biologie Cellulaire and Laboratoire du Metabolisme, Institut National de la Recherche Agronomique, Route de Saint Cyr, 78026 Versailles cedex, France (A.-C.M.A., L.J.)"},{"name":"Laboratoire d'Ecologie Ve\u0301ge\u0301tale, Groupe Photosynthe\u0300se et Environnement, Unite\u0301 de Recherche Associe\u0301e, Centre National de la Recherche Scientifique 2154, ba\u0302t 362, Universite\u0301 de Paris XI, F-91405 Orsay cedex, France (A.-C.M.A., G.C.)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel","family":"Cornic","sequence":"additional","affiliation":[{"name":"Laboratoire d'Ecologie Ve\u0301ge\u0301tale, Groupe Photosynthe\u0300se et Environnement, Unite\u0301 de Recherche Associe\u0301e, Centre National de la Recherche Scientifique 2154, ba\u0302t 362, Universite\u0301 de Paris XI, F-91405 Orsay cedex, France (A.-C.M.A., G.C.)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lise","family":"Jouanin","sequence":"additional","affiliation":[{"name":"Laboratoire de Biologie Cellulaire and Laboratoire du Metabolisme, Institut National de la Recherche Agronomique, Route de Saint Cyr, 78026 Versailles cedex, France (A.-C.M.A., L.J.)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine 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