{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T18:10:47Z","timestamp":1770228647566,"version":"3.49.0"},"reference-count":40,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2021,1,25]],"date-time":"2021-01-25T00:00:00Z","timestamp":1611532800000},"content-version":"vor","delay-in-days":3605,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2011,5,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Trehalose 6-phosphate (T6P) is a sugar signal that regulates metabolism, growth, and development and inhibits the central regulatory SNF1-related protein kinase1 (SnRK1; AKIN10\/AKIN11). To better understand the mechanism in wheat (Triticum aestivum) grain, we analyze T6P content and SnRK1 activities. T6P levels changed 178-fold 1 to 45 d after anthesis (DAA), correlating with sucrose content. T6P ranged from 78 nmol g\u22121 fresh weight (FW) pregrain filling, around 100-fold higher than previously reported in plants, to 0.4 nmol g\u22121 FW during the desiccation stage. In contrast, maximum SnRK1 activity changed only 3-fold but was inhibited strongly by T6P in vitro. To assess SnRK1 activity in vivo, homologs of SnRK1 marker genes in the wheat transcriptome were identified using Wheat Estimated Transcript Server. SnRK1-induced and -repressed marker genes were expressed differently pregrain filling compared to grain filling consistent with changes in T6P. To investigate this further maternal and filial tissues were compared pre- (7 DAA) and during grain filling (17 DAA). Strikingly, in vitro SnRK1 activity was similar in all tissues in contrast to large changes in tissue distribution of T6P. At 7 DAA T6P was 49 to 119 nmol g\u22121 FW in filial and maternal tissues sufficient to inhibit SnRK1; at 17 DAA T6P accumulation was almost exclusively endospermal (43 nmol g\u22121 FW) with 0.6 to 0.8 nmol T6P g\u22121 FW in embryo and pericarp. The data show a correlation between T6P and sucrose overall that belies a marked effect of tissue type and developmental stage on T6P content, consistent with tissue-specific regulation of SnRK1 by T6P in wheat grain.<\/jats:p>","DOI":"10.1104\/pp.111.174524","type":"journal-article","created":{"date-parts":[[2011,3,15]],"date-time":"2011-03-15T20:45:39Z","timestamp":1300221939000},"page":"373-381","source":"Crossref","is-referenced-by-count":168,"title":["Wheat Grain Development Is Characterized by Remarkable Trehalose 6-Phosphate Accumulation Pregrain Filling: Tissue Distribution and Relationship to SNF1-Related Protein Kinase1 Activity\n\u00a0\n\u00a0"],"prefix":"10.1093","volume":"156","author":[{"given":"Eleazar","family":"Mart\u00ednez-Barajas","sequence":"first","affiliation":[{"name":"Plant Science, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, United Kingdom (E.M.-B., C.N., L.F.P., P.C., R.A.C.M., M.J.P.); Molecular Plant Physiology, Utrecht University, 3584\u2013CH Utrecht, The Netherlands (T.D., H.S.); Biomolecular Analysis, Utrecht University, 3584\u2013CG Utrecht, The Netherlands (T.D., G.J.d.J., G.W.S.); Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica, Laborat\u00f3rio de Biotecno"}]},{"given":"Thierry","family":"Delatte","sequence":"additional","affiliation":[{"name":"Plant Science, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, United Kingdom (E.M.-B., C.N., L.F.P., P.C., R.A.C.M., M.J.P.); Molecular Plant Physiology, Utrecht University, 3584\u2013CH Utrecht, The Netherlands (T.D., H.S.); Biomolecular Analysis, Utrecht University, 3584\u2013CG Utrecht, The Netherlands (T.D., G.J.d.J., G.W.S.); Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica, Laborat\u00f3rio de Biotecno"}]},{"given":"Henriette","family":"Schluepmann","sequence":"additional","affiliation":[{"name":"Plant Science, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, United Kingdom (E.M.-B., C.N., L.F.P., P.C., R.A.C.M., M.J.P.); Molecular Plant Physiology, Utrecht University, 3584\u2013CH Utrecht, The Netherlands (T.D., H.S.); Biomolecular Analysis, Utrecht University, 3584\u2013CG Utrecht, The Netherlands (T.D., G.J.d.J., G.W.S.); Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica, Laborat\u00f3rio de Biotecno"}]},{"given":"Gerhardus J.","family":"de Jong","sequence":"additional","affiliation":[{"name":"Plant Science, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, United Kingdom (E.M.-B., C.N., L.F.P., P.C., R.A.C.M., M.J.P.); Molecular Plant Physiology, Utrecht University, 3584\u2013CH Utrecht, The Netherlands (T.D., H.S.); Biomolecular Analysis, Utrecht University, 3584\u2013CG Utrecht, The Netherlands (T.D., G.J.d.J., G.W.S.); Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica, Laborat\u00f3rio de Biotecno"}]},{"given":"Govert W.","family":"Somsen","sequence":"additional","affiliation":[{"name":"Plant Science, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, United Kingdom (E.M.-B., C.N., L.F.P., P.C., R.A.C.M., M.J.P.); Molecular Plant Physiology, Utrecht University, 3584\u2013CH Utrecht, The Netherlands (T.D., H.S.); Biomolecular Analysis, Utrecht University, 3584\u2013CG Utrecht, The Netherlands (T.D., G.J.d.J., G.W.S.); Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica, Laborat\u00f3rio de Biotecno"}]},{"given":"C\u00e1tia","family":"Nunes","sequence":"additional","affiliation":[{"name":"Plant Science, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, United Kingdom (E.M.-B., C.N., L.F.P., P.C., R.A.C.M., M.J.P.); Molecular Plant Physiology, Utrecht University, 3584\u2013CH Utrecht, The Netherlands (T.D., H.S.); Biomolecular Analysis, Utrecht University, 3584\u2013CG Utrecht, The Netherlands (T.D., G.J.d.J., G.W.S.); Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica, Laborat\u00f3rio de Biotecno"}]},{"given":"Lucia F.","family":"Primavesi","sequence":"additional","affiliation":[{"name":"Plant Science, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, United Kingdom (E.M.-B., C.N., L.F.P., P.C., R.A.C.M., M.J.P.); Molecular Plant Physiology, Utrecht University, 3584\u2013CH Utrecht, The Netherlands (T.D., H.S.); Biomolecular Analysis, Utrecht University, 3584\u2013CG Utrecht, The Netherlands (T.D., G.J.d.J., G.W.S.); Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica, Laborat\u00f3rio de Biotecno"}]},{"given":"Patricia","family":"Coello","sequence":"additional","affiliation":[{"name":"Plant Science, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, United Kingdom (E.M.-B., C.N., L.F.P., P.C., R.A.C.M., M.J.P.); Molecular Plant Physiology, Utrecht University, 3584\u2013CH Utrecht, The Netherlands (T.D., H.S.); Biomolecular Analysis, Utrecht University, 3584\u2013CG Utrecht, The Netherlands (T.D., G.J.d.J., G.W.S.); Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica, Laborat\u00f3rio de Biotecno"}]},{"given":"Rowan A.C.","family":"Mitchell","sequence":"additional","affiliation":[{"name":"Plant Science, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, United Kingdom (E.M.-B., C.N., L.F.P., P.C., R.A.C.M., M.J.P.); Molecular Plant Physiology, Utrecht University, 3584\u2013CH Utrecht, The Netherlands (T.D., H.S.); Biomolecular Analysis, Utrecht University, 3584\u2013CG Utrecht, The Netherlands (T.D., G.J.d.J., G.W.S.); Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica, Laborat\u00f3rio de Biotecno"}]},{"given":"Matthew J.","family":"Paul","sequence":"additional","affiliation":[{"name":"Plant Science, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, United Kingdom (E.M.-B., C.N., L.F.P., P.C., R.A.C.M., M.J.P.); Molecular Plant Physiology, Utrecht University, 3584\u2013CH Utrecht, The Netherlands (T.D., H.S.); Biomolecular Analysis, Utrecht University, 3584\u2013CG Utrecht, The Netherlands (T.D., G.J.d.J., G.W.S.); Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica, Laborat\u00f3rio de 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trehalose synthesis, is essential for Arabidopsis embryo maturation","volume":"29","author":"Eastmond","year":"2002","journal-title":"Plant J"},{"key":"2021041906362212200_b7","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/j.plantsci.2005.02.024","article-title":"Biochemical analysis of trehalose and its metabolising enzymes in wheat under abiotic stress conditions","volume":"169","author":"El-Bashiti","year":"2005","journal-title":"Plant Sci"},{"key":"2021041906362212200_b8","doi-asserted-by":"crossref","first-page":"15898","DOI":"10.1073\/pnas.252637799","article-title":"Trehalose accumulation in rice plants confers high tolerance levels to different abiotic stresses","volume":"99","author":"Garg","year":"2002","journal-title":"Proc Natl Acad Sci USA"},{"key":"2021041906362212200_b9","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1007\/s00425-008-0729-x","article-title":"Overexpression of the trehalose-6-phosphate phosphatase gene OsTPP1 confers stress 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