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The interaction of nucleus-localized NPR1 with TGA transcription factors results in the activation of defense genes, including the SAR marker PATHOGENESIS-RELATED-1 (PR-1), and the deployment of SAR. Little is known about how TGA factors or NPR1 regulate transcription or whether a TGA-NPR1 complex forms on DNA. We show that TGA2 and NPR1 are recruited to PR-1 independently of each other and of SA treatment. Consistent with the result that a triple knockout in TGA2\/5\/6 derepresses PR-1, in vivo plant transcription assays revealed that TGA2 is not an autonomous transcription activator but is a transcriptional repressor in both untreated and SA-treated cells. However, after stimulation with SA, TGA2 is incorporated into a transactivating complex with NPR1, forming an enhanceosome that requires the core of the NPR1 BTB\/POZ domain (residues 80 to 91) and the oxidation of NPR1 Cys-521 and Cys-529. These Cys residues are found in a new type of transactivation domain that we term Cys-oxidized. These data further our understanding of the mechanism by which TGA2 and NPR1 activate Arabidopsis PR-1.<\/jats:p>","DOI":"10.1105\/tpc.106.046953","type":"journal-article","created":{"date-parts":[[2006,12,16]],"date-time":"2006-12-16T01:53:49Z","timestamp":1166234029000},"page":"3670-3685","source":"Crossref","is-referenced-by-count":276,"title":["The Coactivator Function of<i>Arabidopsis<\/i>NPR1 Requires the Core of Its BTB\/POZ Domain and the Oxidation of C-Terminal Cysteines"],"prefix":"10.1093","volume":"18","author":[{"given":"Amanda","family":"Rochon","sequence":"first","affiliation":[{"name":"Department of Biological Sciences, Brock University, St. Catharines, Ontario, Canada L2S 3A1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Boyle","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Brock University, St. Catharines, Ontario, Canada L2S 3A1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tracy","family":"Wignes","sequence":"additional","affiliation":[{"name":"National Research Council Canada, Plant Biotechnology Institute, Saskatoon, Saskatchewan, Canada S7N 0W9"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre R.","family":"Fobert","sequence":"additional","affiliation":[{"name":"National Research Council Canada, Plant Biotechnology Institute, Saskatoon, Saskatchewan, Canada S7N 0W9"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles","family":"Despre\u0301s","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Brock University, St. Catharines, Ontario, Canada L2S 3A1"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2006,12,15]]},"reference":[{"key":"2021040619533139200_b1","doi-asserted-by":"crossref","first-page":"1157","DOI":"10.1126\/science.2118682","volume":"249","year":"1990","journal-title":"Science"},{"key":"2021040619533139200_b2","doi-asserted-by":"crossref","first-page":"12123","DOI":"10.1073\/pnas.95.21.12123","volume":"95","year":"1998","journal-title":"Proc. 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