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Ammonium transporter\/methylamine permease\/rhesus (AMT\/Mep\/Rh) transporters are responsible for ammonium acquisition in bacteria, fungi, and plants. The ammonium transporter AMT1;1 from Arabidopsis thaliana uses a novel regulatory mechanism requiring the productive interaction between a trimer of subunits for function. Allosteric regulation is mediated by a cytosolic C-terminal trans-activation domain, which carries a conserved Thr (T460) in a critical position in the hinge region of the C terminus. When expressed in yeast, mutation of T460 leads to inactivation of the trimeric complex. This study shows that phosphorylation of T460 is triggered by ammonium in a time- and concentration-dependent manner. Neither Gln nor l-methionine sulfoximine\u2013induced ammonium accumulation were effective in inducing phosphorylation, suggesting that roots use either the ammonium transporter itself or another extracellular sensor to measure ammonium concentrations in the rhizosphere. Phosphorylation of T460 in response to an increase in external ammonium correlates with inhibition of ammonium uptake into Arabidopsis roots. Thus, phosphorylation appears to function in a feedback loop restricting ammonium uptake. This novel autoregulatory mechanism is capable of tuning uptake capacity over a wide range of supply levels using an extracellular sensory system, potentially mediated by a transceptor (i.e., transporter and receptor).<\/jats:p>","DOI":"10.1105\/tpc.109.068593","type":"journal-article","created":{"date-parts":[[2009,11,30]],"date-time":"2009-11-30T21:34:01Z","timestamp":1259616841000},"page":"3610-3622","source":"Crossref","is-referenced-by-count":195,"title":["Feedback Inhibition of Ammonium Uptake by a Phospho-Dependent Allosteric Mechanism in\n                    <i>Arabidopsis<\/i>\n                    \u00a0"],"prefix":"10.1093","volume":"21","author":[{"given":"Viviane","family":"Lanquar","sequence":"first","affiliation":[{"name":"Department of Plant Biology, Carnegie Institution for Science, Stanford, California 94305"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominique","family":"Loque\u0301","sequence":"additional","affiliation":[{"name":"Department of Plant Biology, Carnegie Institution for Science, Stanford, California 94305"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Friederike","family":"Ho\u0308rmann","sequence":"additional","affiliation":[{"name":"Department of Plant Biology, Carnegie Institution for Science, Stanford, California 94305"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lixing","family":"Yuan","sequence":"additional","affiliation":[{"name":"Key Lab of Plant Nutrition, College of Resources and Environmental Sciences, China Agricultural University, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Bohner","sequence":"additional","affiliation":[{"name":"Molecular Plant Nutrition, Leibniz-Institute for Plant Genetics and Crop Plant Research, 06466 Gatersleben, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wolfgang R.","family":"Engelsberger","sequence":"additional","affiliation":[{"name":"Max Planck Institut fu\u0308r Molekulare Pflanzenphysiologie, 14476 Golm, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvie","family":"Lalonde","sequence":"additional","affiliation":[{"name":"Department of Plant Biology, Carnegie Institution for Science, Stanford, California 94305"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Waltraud X.","family":"Schulze","sequence":"additional","affiliation":[{"name":"Max Planck Institut fu\u0308r Molekulare Pflanzenphysiologie, 14476 Golm, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolaus","family":"von Wire\u0301n","sequence":"additional","affiliation":[{"name":"Molecular Plant Nutrition, Leibniz-Institute for Plant Genetics and Crop Plant Research, 06466 Gatersleben, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wolf B.","family":"Frommer","sequence":"additional","affiliation":[{"name":"Department of Plant Biology, Carnegie Institution for Science, Stanford, California 94305"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2009,11,30]]},"reference":[{"key":"2021040621173530900_b1","doi-asserted-by":"crossref","first-page":"14994","DOI":"10.1073\/pnas.0506254102","volume":"102","year":"2005","journal-title":"Proc. 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