{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,3,4]],"date-time":"2025-03-04T05:35:31Z","timestamp":1741066531789,"version":"3.38.0"},"reference-count":32,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2001,10,1]],"date-time":"2001-10-01T00:00:00Z","timestamp":1001894400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Food sci. technol. int."],"published-print":{"date-parts":[[2001,10]]},"abstract":"<jats:p> The thermal inactivation of horseradish peroxidase freeze-dried from solutions of different pH (8, 10 and 11.5, measured at 25 C) and equilibrated to different water contents was studied in the temperature range from 110 to 150 C. The water contents studied (0.0, 1.4, 16.2 and 25.6 g water per 100 g of dry enzyme) corresponded to water activities of 0.0, 0.11, 0.76 and 0.88 at 4 C. The kinetics were well described by a double exponential model. The enzyme was generally more stable the lower the pH of the original solution, and for all pH values, the maximum stability was obtained at 1.4 g water\/100 g dry enzyme. Values of z were generally independent of water content and of the pH of the original solution, and in the range of 15\u201325 \u00b0C, usually found in neutral conditions, with the exception of the enzyme freeze dried from pH 11.5 and equilibrated with phosphorus pentoxide, where a z-value of the stable fraction close to 10 C was found. <\/jats:p>","DOI":"10.1106\/e9nw-1tb4-ku4w-8x7m","type":"journal-article","created":{"date-parts":[[2002,8,24]],"date-time":"2002-08-24T21:38:13Z","timestamp":1030225093000},"page":"393-398","source":"Crossref","is-referenced-by-count":0,"title":["Effect of Water Content on the Thermal Inactivation Kinetics of Horseradish Peroxidase Freeze-Dried from Alkaline pH"],"prefix":"10.1177","volume":"7","author":[{"given":"M. A.","family":"Lemos","sequence":"first","affiliation":[{"name":"Escola Superior de Biotecnologia, Universidade Cat\u00f3lica Portuguesa, Rua Dr. Ant\u00f3nio Bernardino de Almeida, 4200 Porto, Portugal"}]},{"given":"J. C.","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Department of Food Science and Technology, University College Cork, Cork, Ireland"}]},{"given":"J. A.","family":"Saraiva","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Aveiro, Campus de Santiago, 3810-193 Aveiro, Portugal"}]}],"member":"179","published-online":{"date-parts":[[2001,10,1]]},"reference":[{"key":"atypb1","doi-asserted-by":"publisher","DOI":"10.1016\/S0308-8146(96)00320-2"},{"key":"atypb2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2621.1977.tb01204.x"},{"key":"atypb3","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2621.1988.tb08986.x"},{"key":"atypb4","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0290(19970205)53:3<345::AID-BIT14>3.0.CO;2-J"},{"key":"atypb5","doi-asserted-by":"publisher","DOI":"10.1016\/S0308-8146(98)00177-0"},{"key":"atypb6","doi-asserted-by":"publisher","DOI":"10.1104\/pp.4.4.537"},{"key":"atypb7","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2621.1991.tb07972.x"},{"key":"atypb8","doi-asserted-by":"publisher","DOI":"10.6028\/jres.081A.011"},{"key":"atypb9","doi-asserted-by":"publisher","DOI":"10.1006\/fstl.1993.1080"},{"key":"atypb10","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1111\/j.1365-2621.1976.tb00755.x","volume":"41","author":"H\u00e4gerdal B.","year":"1976","journal-title":"Journal of Food Science"},{"key":"atypb11","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2621.1992.tb01175.x"},{"key":"atypb12","doi-asserted-by":"publisher","DOI":"10.1016\/0141-0229(85)90013-4"},{"key":"atypb13","doi-asserted-by":"publisher","DOI":"10.1038\/374596a0"},{"key":"atypb14","doi-asserted-by":"publisher","DOI":"10.1080\/08905439609549959"},{"key":"atypb15","doi-asserted-by":"publisher","DOI":"10.1006\/fstl.2000.0694"},{"key":"atypb16","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2621.1978.tb15295.x"},{"key":"atypb17","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2621.1995.tb09801.x"},{"key":"atypb18","doi-asserted-by":"publisher","DOI":"10.1016\/0260-8774(91)90001-9"},{"key":"atypb19","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-223150-6.50027-4"},{"issue":"3","key":"atypb20","first-page":"279","volume":"22","author":"Pizzocaro F.","year":"1993","journal-title":"Rivista di Scienza dell'Alimentazione"},{"key":"atypb21","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-59.10.1065"},{"key":"atypb22","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-60.8.967"},{"key":"atypb23","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2795(75)90188-9"},{"key":"atypb24","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2621.1996.00342.x"},{"key":"atypb25","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2621.1996.00343.x"},{"key":"atypb26","doi-asserted-by":"publisher","DOI":"10.1016\/S1043-4526(08)60084-4"},{"key":"atypb27","doi-asserted-by":"publisher","DOI":"10.1016\/0924-2244(96)81353-7"},{"key":"atypb28","first-page":"13","volume-title":"Processing of Foods: Quality Optimisation and Process Assessment","author":"Van Loey A.","year":"1999"},{"key":"atypb29","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2621.1991.tb05328.x"},{"key":"atypb30","first-page":"8","volume-title":"Principles of Enzymology for the Food Sciences","author":"Whitaker J. 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