{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T23:37:07Z","timestamp":1779233827749,"version":"3.51.4"},"reference-count":0,"publisher":"International Union of Crystallography (IUCr)","issue":"10","license":[{"start":{"date-parts":[[2006,9,19]],"date-time":"2006-09-19T00:00:00Z","timestamp":1158624000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/2.0\/uk\/legalcode"},{"start":{"date-parts":[[2006,9,19]],"date-time":"2006-09-19T00:00:00Z","timestamp":1158624000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/2.0\/uk\/legalcode"}],"content-domain":{"domain":["iucr.org","wiley.com","iucrj.org"],"crossmark-restriction":true},"short-container-title":["Acta Crystallogr D Biol Crystallogr","Acta Cryst D","Acta Cryst Sect D","Acta Crystallogr D","Acta Crystallogr Sect D","Acta Crystallogr Sect D Biol Crystallogr","Acta Crystallogr D Biol Cryst"],"accepted":{"date-parts":[[2006,8,1]]},"published-print":{"date-parts":[[2006,10,1]]},"abstract":"<jats:p>Epstein\u2013Barr virus is a herpesvirus that causes infectious mononucleosis, carcinomas and immunoproliferative disease. Its genome encodes 86 proteins, which provided targets for a structural genomics project. After updating the annotation of the genome, 23 open reading frames were chosen for expression in <jats:italic>Escherichia coli<\/jats:italic>, initially selecting for those with known enzyme activity and then supplementing this set based on a series of predicted properties, in particular secondary structure. The major obstacle turned out to be poor expression and low solubility. Surprisingly, this could not be overcome by modifications of the constructs, changes of expression temperature or strain or renaturation. Of the eight soluble proteins, five were crystallized using robotic nanolitre-drop crystallization trials, which led to four solved structures. Although these results depended on individual treatment rather than standardized protocols, a high-throughput miniaturized crystallization screening protocol was a key component of success with these difficult proteins.<\/jats:p>","DOI":"10.1107\/s0907444906030034","type":"journal-article","created":{"date-parts":[[2006,9,26]],"date-time":"2006-09-26T11:17:48Z","timestamp":1159269468000},"page":"1276-1285","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":35,"title":["Structural genomics of the Epstein\u2013Barr virus"],"prefix":"10.1107","volume":"62","author":[{"given":"Nicolas","family":"Tarbouriech","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marlyse","family":"Buisson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thibault","family":"G\u00e9oui","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susan","family":"Daenke","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen","family":"Cusack","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wim Pascal","family":"Burmeister","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"329","published-online":{"date-parts":[[2006,9,19]]},"container-title":["Acta Crystallographica Section D Biological Crystallography"],"original-title":[],"link":[{"URL":"http:\/\/journals.iucr.org\/d\/issues\/2006\/10\/00\/gx5078\/gx5078.pdf","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/journals.iucr.org\/d\/issues\/2006\/10\/00\/gx5078\/gx5078.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,11]],"date-time":"2025-01-11T14:23:02Z","timestamp":1736605382000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.iucr.org\/paper?S0907444906030034"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,9,19]]},"references-count":0,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2006,10]]}},"alternative-id":["S0907444906030034"],"URL":"https:\/\/doi.org\/10.1107\/s0907444906030034","relation":{},"ISSN":["0907-4449"],"issn-type":[{"value":"0907-4449","type":"print"}],"subject":[],"published":{"date-parts":[[2006,9,19]]},"assertion":[{"value":"Acta Crystallographica Section D: Biological Crystallography","order":0,"name":"journal","label":"Publication","explanation":{"URL":"http:\/\/journals.iucr.org\/d"},"group":{"name":"general","label":"General"}},{"value":"research papers","order":1,"name":"content_type","label":"Content type","explanation":{"URL":"https:\/\/journals.iucr.org\/d\/services\/notesforauthors.html"},"group":{"name":"general","label":"General"}},{"value":"Available","URL":"https:\/\/scripts.iucr.org\/cgi-bin\/citedin?gx5078","order":2,"name":"article_metrics","label":"Article metrics","group":{"name":"general","label":"General"}},{"value":"Yes","order":0,"name":"peer_reviewed","label":"Peer reviewed","group":{"name":"peer_review","label":"Peer review"}},{"value":"Single blind","order":1,"name":"review_process","label":"Review process","group":{"name":"peer_review","label":"Peer review"}},{"value":"15 November 2005","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication history"}},{"value":"1 August 2006","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication history"}},{"value":"19 September 2006","order":2,"name":"published_online","label":"Published online","group":{"name":"publication_history","label":"Publication history"}},{"value":"\u00a9 2006 International Union of Crystallography","order":0,"name":"copyright","label":"Copyright","explanation":{"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},"group":{"name":"copyright_and_licensing","label":"Copyright and licencing"}}]}}