{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T16:11:31Z","timestamp":1762099891043,"version":"3.32.0"},"reference-count":0,"publisher":"International Union of Crystallography (IUCr)","issue":"6","license":[{"start":{"date-parts":[[1998,11,1]],"date-time":"1998-11-01T00:00:00Z","timestamp":909878400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},{"start":{"date-parts":[[1998,11,1]],"date-time":"1998-11-01T00:00:00Z","timestamp":909878400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html#TDM"}],"content-domain":{"domain":["iucr.org","wiley.com","iucrj.org"],"crossmark-restriction":true},"short-container-title":["Acta Crystallogr D Biol Crystallogr","Acta Cryst D","Acta Cryst Sect D","Acta Crystallogr D","Acta Crystallogr Sect D","Acta Crystallogr Sect D Biol Crystallogr","Acta Crystallogr D Biol Cryst"],"accepted":{"date-parts":[[1998,4,6]]},"published-print":{"date-parts":[[1998,11,1]]},"abstract":"<jats:p>The \u03b1-toxin of <jats:italic>Clostridium perfringens<\/jats:italic> is the major virulence determinant for gas gangrene in man. The gene encoding the \u03b1-toxin has been cloned into <jats:italic>E. coli<\/jats:italic> from two strains of the bacterium (NCTC8237 and CER89L43) and subsequently purified to homogeneity. The two strains of \u03b1-toxin differ by five amino acids, resulting in the toxin from NCTC8237 being sensitive to chymotrypsin digestion while that from CER89L43 is resistant. The \u03b1-toxin from each of these strains has been crystallized in two different forms by the hanging-drop vapour-diffusion method at 293\u2005K. CER89L43 form I crystals belong to space group <jats:italic>R<\/jats:italic>32 and have two molecules in the crystallographic asymmetric unit and a unit cell with <jats:italic>a<\/jats:italic> = <jats:italic>b<\/jats:italic> = 151.4, <jats:italic>c<\/jats:italic> = 195.5\u2005\u00c5, \u03b1 = \u03b2 = 90, \u03b3 = 120\u00b0. The crystals diffracted to <jats:italic>d<\/jats:italic>\n                  <jats:sub>min<\/jats:sub> = 1.90\u2005\u00c5. The characteristics of the NCTC8237 form I crystals have already been reported. The form II crystals from both strains belong to space group <jats:italic>C<\/jats:italic>222<jats:sub>1<\/jats:sub> with one molecule in the crystallographic asymmetric unit and, for strain CER89L43, have cell dimensions <jats:italic>a<\/jats:italic> = 61.05, <jats:italic>b<\/jats:italic> = 177.50, <jats:italic>c<\/jats:italic> = 79.05\u2005\u00c5, \u03b1 = \u03b2 = \u03b3 = 90\u00b0, while for strain NCTC8237 the cell dimensions are <jats:italic>a<\/jats:italic> = 60.50, <jats:italic>b<\/jats:italic> = 175.70, <jats:italic>c<\/jats:italic> = 80.20\u2005\u00c5, \u03b1 = \u03b2 = \u03b3 = 90\u00b0. The crystals diffracted to maximum resolutions of 1.85 and 2.1\u2005\u00c5 for the CER89L43 and the NCTC8237 strains, respectively.<\/jats:p>","DOI":"10.1107\/s0907444998005186","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T05:23:52Z","timestamp":1027747432000},"page":"1425-1428","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":4,"special_numbering":"2","title":["Crystallization and preliminary X-ray diffraction studies of \u03b1-toxin from two different strains (NCTC8237 and CER89L43) of <i>Clostridium perfringens<\/i>"],"prefix":"10.1107","volume":"54","author":[{"given":"A. K.","family":"Basak","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Howells","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. T.","family":"Eaton","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. S.","family":"Moss","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. E.","family":"Naylor","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Miller","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. W.","family":"Titball","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"329","published-online":{"date-parts":[[1998,11,1]]},"container-title":["Acta Crystallographica Section D Biological Crystallography"],"original-title":[],"link":[{"URL":"http:\/\/journals.iucr.org\/d\/issues\/1998\/06\/02\/ad0044\/ad0044.pdf","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/journals.iucr.org\/d\/issues\/1998\/06\/02\/ad0044\/ad0044.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,10]],"date-time":"2025-01-10T13:06:08Z","timestamp":1736514368000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.iucr.org\/paper?S0907444998005186"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,11,1]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1998,11]]}},"alternative-id":["S0907444998005186"],"URL":"https:\/\/doi.org\/10.1107\/s0907444998005186","relation":{},"ISSN":["0907-4449"],"issn-type":[{"type":"print","value":"0907-4449"}],"subject":[],"published":{"date-parts":[[1998,11,1]]},"assertion":[{"value":"Acta Crystallographica Section D: Biological Crystallography","order":0,"name":"journal","label":"Publication","explanation":{"URL":"http:\/\/journals.iucr.org\/d"},"group":{"name":"general","label":"General"}},{"value":"crystallization papers","order":1,"name":"content_type","label":"Content type","explanation":{"URL":"https:\/\/journals.iucr.org\/d\/services\/notesforauthors.html"},"group":{"name":"general","label":"General"}},{"value":"Available","URL":"https:\/\/scripts.iucr.org\/cgi-bin\/citedin?ad0044","order":2,"name":"article_metrics","label":"Article metrics","group":{"name":"general","label":"General"}},{"value":"Yes","order":0,"name":"peer_reviewed","label":"Peer reviewed","group":{"name":"peer_review","label":"Peer review"}},{"value":"Single blind","order":1,"name":"review_process","label":"Review process","group":{"name":"peer_review","label":"Peer review"}},{"value":"2 December 1997","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication history"}},{"value":"6 April 1998","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication history"}},{"value":"1 November 1998","order":2,"name":"published_online","label":"Published online","group":{"name":"publication_history","label":"Publication history"}},{"value":"\u00a9 1998 International Union of Crystallography","order":0,"name":"copyright","label":"Copyright","explanation":{"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},"group":{"name":"copyright_and_licensing","label":"Copyright and licencing"}}]}}