{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,1,12]],"date-time":"2025-01-12T05:12:00Z","timestamp":1736658720801,"version":"3.32.0"},"reference-count":0,"publisher":"International Union of Crystallography (IUCr)","issue":"2","license":[{"start":{"date-parts":[[1999,2,1]],"date-time":"1999-02-01T00:00:00Z","timestamp":917827200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},{"start":{"date-parts":[[1999,2,1]],"date-time":"1999-02-01T00:00:00Z","timestamp":917827200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html#TDM"}],"content-domain":{"domain":["iucr.org","wiley.com","iucrj.org"],"crossmark-restriction":true},"short-container-title":["Acta Crystallogr D Biol Crystallogr","Acta Cryst D","Acta Cryst Sect D","Acta Crystallogr D","Acta Crystallogr Sect D","Acta Crystallogr Sect D Biol Crystallogr","Acta Crystallogr D Biol Cryst"],"published-print":{"date-parts":[[1999,2,1]]},"abstract":"<jats:p>UDP-galactopyranose mutase, the enzyme responsible for the conversion of UDP-galactopyranose to UDP-galactofuranose, has been crystallized in a form suitable for X-ray diffraction studies. UDP-galactofuranose is a key component of mycobacterial cell walls. Crystals of both the native protein and a selenomethionine variant have been grown by the vapour-diffusion method in hanging drops, and diffract to beyond 3.0\u2005\u00c5 using synchrotron radiation. Equilibration was against a solution of 20%(<jats:italic>w<\/jats:italic>\/<jats:italic>v<\/jats:italic>) polyethylene glycol (4K), 12%(<jats:italic>v<\/jats:italic>\/<jats:italic>v<\/jats:italic>) 2-propanol, 0.1\u2005<jats:italic>M<\/jats:italic> HEPES pH 7.6 at 293.5\u2005K. Crystals grow as thin plates of dimensions 0.4 \u00d7 0.2 \u00d7 \u223c0.02\u2005mm. They are orthorhombic, space group <jats:italic>P<\/jats:italic>2<jats:sub>1<\/jats:sub>, with unit-cell dimensions <jats:italic>a<\/jats:italic>\u00a0=\u00a071.12, <jats:italic>b<\/jats:italic> = 58.42, <jats:italic>c<\/jats:italic>\u00a0=\u00a096.38\u2005\u00c5, \u03b2 = 96.38\u00b0. 92% (native) and 94% (selenomethionine) complete data sets have been recorded to 2.9\u2005\u00c5 (<jats:italic>R<\/jats:italic>\n                  <jats:sub>merge<\/jats:sub> = 5.0%) and 3.0\u2005\u00c5 (<jats:italic>R<\/jats:italic>\n                  <jats:sub>merge<\/jats:sub> = 6.9%), respectively. The Matthews coefficient is 2.35\u2005\u00c5<jats:sup>3<\/jats:sup>\u2005Da<jats:sup>\u22121<\/jats:sup> for a dimer in the asymmetric unit, the solvent content being 47%. Diffraction data have also been recorded on a putative platinum derivative to 3.5\u2005\u00c5.<\/jats:p>","DOI":"10.1107\/s0907444998010877","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T05:48:20Z","timestamp":1027748900000},"page":"399-402","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":8,"title":["Initiating a crystallographic study of UDP-galactopyranose mutase from <i>Escherichia coli<\/i>"],"prefix":"10.1107","volume":"55","author":[{"given":"Stephen A.","family":"McMahon","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gordon A.","family":"Leonard","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Louise V.","family":"Buchanan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marie-France","family":"Giraud","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James H.","family":"Naismith","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"329","published-online":{"date-parts":[[1999,2,1]]},"container-title":["Acta Crystallographica Section D Biological Crystallography"],"original-title":[],"link":[{"URL":"http:\/\/journals.iucr.org\/d\/issues\/1999\/02\/00\/li0300\/li0300.pdf","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/journals.iucr.org\/d\/issues\/1999\/02\/00\/li0300\/li0300.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,11]],"date-time":"2025-01-11T12:41:53Z","timestamp":1736599313000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.iucr.org\/paper?S0907444998010877"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1999,2,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1999,2]]}},"alternative-id":["S0907444998010877"],"URL":"https:\/\/doi.org\/10.1107\/s0907444998010877","relation":{},"ISSN":["0907-4449"],"issn-type":[{"type":"print","value":"0907-4449"}],"subject":[],"published":{"date-parts":[[1999,2,1]]},"assertion":[{"value":"Acta Crystallographica Section D: Biological Crystallography","order":0,"name":"journal","label":"Publication","explanation":{"URL":"http:\/\/journals.iucr.org\/d"},"group":{"name":"general","label":"General"}},{"value":"research papers","order":1,"name":"content_type","label":"Content type","explanation":{"URL":"http:\/\/journals.iucr.org\/d\/services\/notesforauthors.html"},"group":{"name":"general","label":"General"}},{"value":"Available","URL":"http:\/\/scripts.iucr.org\/cgi-bin\/citedin?li0300","order":2,"name":"article_metrics","label":"Article metrics","group":{"name":"general","label":"General"}},{"value":"Yes","order":0,"name":"peer_reviewed","label":"Peer reviewed","group":{"name":"peer_review","label":"Peer review"}},{"value":"Single blind","order":1,"name":"review_process","label":"Review process","group":{"name":"peer_review","label":"Peer review"}},{"value":"9 July 1998","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication history"}},{"value":"14 August 1998","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication history"}},{"value":"1 February 1999","order":2,"name":"published_online","label":"Published online","group":{"name":"publication_history","label":"Publication history"}},{"value":"\u00a9 1999 International Union of Crystallography","order":0,"name":"copyright","label":"Copyright","explanation":{"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},"group":{"name":"copyright_and_licensing","label":"Copyright and licencing"}}]}}