{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T04:09:02Z","timestamp":1790827742892,"version":"4.1.0"},"reference-count":51,"publisher":"International Union of Crystallography (IUCr)","issue":"11","license":[{"start":{"date-parts":[[2014,10,23]],"date-time":"2014-10-23T00:00:00Z","timestamp":1414022400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},{"start":{"date-parts":[[2014,10,23]],"date-time":"2014-10-23T00:00:00Z","timestamp":1414022400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html#TDM"}],"content-domain":{"domain":["iucr.org","wiley.com","iucrj.org"],"crossmark-restriction":true},"short-container-title":["Acta Crystallogr D Biol Crystallogr","Acta Crystallogr D","Acta Crystallogr D Biol Cryst","Acta Cryst D","Acta Cryst D Biol Crystallogr","Acta Cryst D Biol Cryst","Acta Cryst Sect D","Acta Cryst Sect D Biol Cryst","Acta Crystallogr Sect D","Acta Crystallogr Sect D Biol Crystallogr"],"published-print":{"date-parts":[[2014,11,1]]},"abstract":"<jats:p>Outbreaks of multidrug-resistant bacterial infections have become more frequent worldwide owing to the emergence of several different classes of \u03b2-lactamases. In this study, the molecular, biochemical and structural characteristics of an<jats:italic>Acinetobacter<\/jats:italic>-derived cephalosporinase (ADC)-type class C \u03b2-lactamase, ADC-68, isolated from the carbapenem-resistant<jats:italic>A. baumannii<\/jats:italic>D015 were investigated. The<jats:italic>bla<\/jats:italic><jats:sub>ADC-68<\/jats:sub>gene which encodes ADC-68 was confirmed to exist on the chromosome<jats:italic>via<\/jats:italic>Southern blot analysis and draft genome sequencing. The catalytic kinetics of \u03b2-lactams and their MICs (minimum inhibitory concentrations) for<jats:italic>A. baumannii<\/jats:italic>D015 and purified ADC-68 (a carbapenemase obtained from this strain) were assessed: the strain was resistant to penicillins, narrow-spectrum and extended-spectrum cephalosporins, and carbapenems, which were hydrolyzed by ADC-68. The crystal structure of ADC-68 was determined at a resolution of 1.8\u2005\u00c5. The structure of ADC-68 was compared with that of ADC-1 (a non-carbapenemase); differences were found in the central part of the \u03a9-loop and the C-loop constituting the edge of the R1 and R2 subsites and are close to the catalytic serine residue Ser66. The ADC-68 C-loop was stabilized in the open conformation of the upper R2 subsite and could better accommodate carbapenems with larger<jats:italic>R<\/jats:italic><jats:sub>2<\/jats:sub>side chains. Furthermore, a wide-open conformation of the R2-loop allowed ADC-68 to bind to and hydrolyze extended-spectrum cephalosporins. Therefore, ADC-68 had enhanced catalytic efficiency against these clinically important \u03b2-lactams (extended-spectrum cephalosporins and carbapenems). ADC-68 is the first reported enzyme among the chromosomal class C \u03b2-lactamases to possess class C extended-spectrum \u03b2-lactamase and carbapenemase activities.<\/jats:p>","DOI":"10.1107\/s1399004714019543","type":"journal-article","created":{"date-parts":[[2014,10,22]],"date-time":"2014-10-22T15:40:56Z","timestamp":1413992456000},"page":"2924-2936","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":46,"title":["Structure of ADC-68, a novel carbapenem-hydrolyzing class C extended-spectrum \u03b2-lactamase isolated from<i>Acinetobacter baumannii<\/i>"],"prefix":"10.1107","volume":"70","author":[{"given":"Jeong Ho","family":"Jeon","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Myoung-Ki","family":"Hong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jung Hun","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jae Jin","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kwang Seung","family":"Park","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asad Mustafa","family":"Karim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeong Yeon","family":"Jo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji Hwan","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kwan Soo","family":"Ko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin-Woo","family":"Kang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sang Hee","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"329","published-online":{"date-parts":[[2014,10,23]]},"reference":[{"key":"dw5111_bb1","doi-asserted-by":"crossref","first-page":"576","DOI":"10.1093\/jac\/41.5.576","volume":"41","author":"Afzal-Shah","year":"1998","journal-title":"J. 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