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The O<jats:sub>2<\/jats:sub>gas was made from the reaction between H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>and catalase. The gas production was imaged by PCI in real time. The H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>was injected into the enteric cavity of the lower sigmoid colon to produce O<jats:sub>2<\/jats:sub>in the submucosal venous plexus. The generated O<jats:sub>2<\/jats:sub>gas could be finally drained into hepatic portal veins. Absorption contrast imaging (ACI) and PCI of O<jats:sub>2<\/jats:sub>-filled portal veins were performed and compared. PCI offers high resolution and real-time visualization of the O<jats:sub>2<\/jats:sub>gas production. Compared with O<jats:sub>2<\/jats:sub>-based ACI, O<jats:sub>2<\/jats:sub>-based PCI significantly enhanced the revealing of the portal vein<jats:italic>in vivo<\/jats:italic>. It is concluded that O<jats:sub>2<\/jats:sub>-based PCI is a novel and promising imaging modality for future studies of portal venous disorders in mice models.<\/jats:p>","DOI":"10.1107\/s1600577515010103","type":"journal-article","created":{"date-parts":[[2015,7,13]],"date-time":"2015-07-13T15:32:18Z","timestamp":1436801538000},"page":"1263-1267","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":2,"title":["A novel imaging tool for hepatic portal system using phase contrast technique with hydrogen peroxide-generated O<sub>2<\/sub>gas"],"prefix":"10.1107","volume":"22","author":[{"given":"Rongbiao","family":"Tang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fuhua","family":"Yan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei-Min","family":"Chai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanan","family":"Fu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guo-Yuan","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ke-Min","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"329","published-online":{"date-parts":[[2015,7,14]]},"reference":[{"key":"mo5112_bb1","doi-asserted-by":"crossref","first-page":"R1","DOI":"10.1088\/0031-9155\/58\/1\/R1","volume":"58","author":"Bravin","year":"2013","journal-title":"Phys. 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