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Nevertheless, the associated radiation damage impacts on\u00a0the integrity of delicate biological samples. Herein, the extent of soft X-ray radiation damage in popular thin freeze-dried brain tissue samples mounted onto Si<jats:sub>3<\/jats:sub>N<jats:sub>4<\/jats:sub> membranes, as highlighted by Fourier transform infrared microscopy (FTIR), is reported. The freeze-dried tissue samples were found to be affected by general degradation of the vibrational architecture, though these effects were weaker than those observed in paraffin-embedded and hydrated systems reported in the literature. In addition, weak, reversible and specific features of the tissue\u2013Si<jats:sub>3<\/jats:sub>N<jats:sub>4<\/jats:sub> interaction could be identified for the first time upon routine soft X-ray exposures, further highlighting the complex interplay between the biological sample, its preparation protocol and X-ray probe.<\/jats:p>","DOI":"10.1107\/s1600577520010103","type":"journal-article","created":{"date-parts":[[2020,8,21]],"date-time":"2020-08-21T11:07:41Z","timestamp":1598008061000},"page":"1218-1226","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":10,"title":["Soft X-ray induced radiation damage in thin freeze-dried brain samples studied by FTIR microscopy"],"prefix":"10.1107","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2430-7958","authenticated-orcid":true,"given":"Artur D.","family":"Surowka","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2457-3618","authenticated-orcid":true,"given":"A.","family":"Gianoncelli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2418-058X","authenticated-orcid":true,"given":"G.","family":"Birarda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1949-0877","authenticated-orcid":true,"given":"S.","family":"Sala","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5429-058X","authenticated-orcid":true,"given":"N.","family":"Cefarin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Matruglio","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8331-1917","authenticated-orcid":true,"given":"M.","family":"Szczerbowska-Boruchowska","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Ziomber-Lisiak","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2355-114X","authenticated-orcid":true,"given":"L.","family":"Vaccari","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"329","published-online":{"date-parts":[[2020,8,21]]},"reference":[{"key":"hf5398_bb1","doi-asserted-by":"crossref","first-page":"4523","DOI":"10.1021\/jp200947g","volume":"115","author":"Alizadeh","year":"2011","journal-title":"J. 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