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Following exposure to synchrotron-sourced (SS) terahertz (THz) radiation, reversible membrane permeability has been demonstrated in eukaryotic cells by the uptake of nanospheres; nonetheless, cellular localization of the nanospheres remained unclear. This study utilized silica core-shell gold nanospheres (AuSi NS) of diameter 50 \u00b1 5\u2005nm to investigate the fate of nanospheres inside pheochromocytoma (PC\u200512) cells following SS\u2005THz exposure. Fluorescence microscopy was used to confirm nanosphere internalization following 10\u2005min of SS\u2005THz exposure in the range 0.5\u201320\u2005THz. Transmission electron microscopy followed by scanning transmission electron microscopy energy-dispersive spectroscopic (STEM-EDS) analysis was used to confirm the presence of AuSi NS in the cytoplasm or membrane, as single NS or in clusters (22% and 52%, respectively), with the remainder (26%) sequestered in vacuoles. 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