{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,1,12]],"date-time":"2025-01-12T05:18:40Z","timestamp":1736659120932,"version":"3.32.0"},"reference-count":14,"publisher":"International Union of Crystallography (IUCr)","issue":"9","license":[{"start":{"date-parts":[[2010,8,28]],"date-time":"2010-08-28T00:00:00Z","timestamp":1282953600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},{"start":{"date-parts":[[2010,8,28]],"date-time":"2010-08-28T00:00:00Z","timestamp":1282953600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html#TDM"}],"content-domain":{"domain":["iucr.org","wiley.com","iucrj.org"],"crossmark-restriction":true},"short-container-title":["Acta Crystallogr F Struct Biol Cryst Commun","Acta Cryst F","Acta Cryst Sect F","Acta Crystallogr F","Acta Crystallogr Sect F","Acta Cryst F Struct Biol Cryst Commun","Acta Cryst Sect F Struct Biol Cryst Commun"],"published-print":{"date-parts":[[2010,9,1]]},"abstract":"<jats:p>FILIA is a component of the subcortical maternal complex that is essential for early stage embryogenesis. Its 6\u00d7His-tagged N-terminal domain was expressed in<jats:italic>Escherichia coli<\/jats:italic>and purified to homogeneity. Two types of crystals formed under different crystallization conditions during screening. Orthorhombic crystals appeared in a solution containing 1.4\u2005<jats:italic>M<\/jats:italic>ammonium sulfate, 0.1\u2005<jats:italic>M<\/jats:italic>Tris pH 8.2 and 12% glycerol, while tetragonal crystals were obtained using 15% PEG 4000 mixed with 0.1\u2005<jats:italic>M<\/jats:italic>HEPES pH 7.5 and 15% 2-propanol. High-quality diffraction data were collected from the two crystal forms to resolutions of 1.8 and 2.2\u2005\u00c5, respectively, using synchrotron radiation. The Matthews coefficients indicated that the<jats:italic>P<\/jats:italic>2<jats:sub>1<\/jats:sub>2<jats:sub>1<\/jats:sub>2<jats:sub>1<\/jats:sub>and<jats:italic>P<\/jats:italic>4<jats:sub>1<\/jats:sub>2<jats:sub>1<\/jats:sub>2 crystals contained two molecules and one molecule per asymmetric unit, respectively. A selenomethionine-substituted sample failed to crystallize under the native conditions, but another orthorhombic crystal form was obtained under different conditions and anomalous diffraction data were collected.<\/jats:p>","DOI":"10.1107\/s1744309110031994","type":"journal-article","created":{"date-parts":[[2010,8,27]],"date-time":"2010-08-27T16:18:12Z","timestamp":1282925892000},"page":"1111-1114","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":0,"title":["Preliminary crystallographic analysis of the N-terminal domain of FILIA, a protein essential for embryogenesis"],"prefix":"10.1107","volume":"66","author":[{"given":"Juke","family":"Wang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tong-Cun","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinqi","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"329","published-online":{"date-parts":[[2010,8,28]]},"reference":[{"key":"hc5115_bb1","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1107\/S0907444909052925","volume":"66","author":"Adams","year":"2010","journal-title":"Acta Cryst. 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