{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T12:34:40Z","timestamp":1770899680001,"version":"3.50.1"},"reference-count":45,"publisher":"International Union of Crystallography (IUCr)","issue":"12","license":[{"start":{"date-parts":[[2011,11,26]],"date-time":"2011-11-26T00:00:00Z","timestamp":1322265600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},{"start":{"date-parts":[[2011,11,26]],"date-time":"2011-11-26T00:00:00Z","timestamp":1322265600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html#TDM"}],"content-domain":{"domain":["iucr.org","wiley.com","iucrj.org"],"crossmark-restriction":true},"short-container-title":["Acta Crystallogr F Struct Biol Cryst Commun","Acta Cryst F","Acta Cryst Sect F","Acta Crystallogr F","Acta Crystallogr Sect F","Acta Cryst F Struct Biol Cryst Commun","Acta Cryst Sect F Struct Biol Cryst Commun"],"accepted":{"date-parts":[[2011,10,1]]},"published-print":{"date-parts":[[2011,12,1]]},"abstract":"<jats:p>Penicillin G acylase from <jats:italic>Bacillus megaterium<\/jats:italic> (<jats:italic>Bm<\/jats:italic>PGA) is currently used in the\u00a0pharmaceutical industry as an alternative to PGA from <jats:italic>Escherichia coli<\/jats:italic> (<jats:italic>Ec<\/jats:italic>PGA) for the hydrolysis of penicillin G to produce 6-aminopenicillanic acid (6-APA), a penam nucleus for semisynthetic penicillins. Despite the significant differences in amino-acid sequence between PGAs from Gram-positive and Gram-negative bacteria, a representative PGA structure of Gram-positive origin has never been reported. In this study, crystallization and diffraction studies of <jats:italic>Bm<\/jats:italic>PGA are described. Poor diffraction patterns with blurred spots at\u00a0higher resolution were typical for <jats:italic>Bm<\/jats:italic>PGA crystals cryocooled after a brief immersion in cryoprotectant solution. Overnight soaking in the same cryo-solution substantially improved both the mosaicity and resolution limit through the establishment of a new crystal-packing equilibrium. A crystal of <jats:italic>Bm<\/jats:italic>PGA diffracted X-rays to 2.20\u2005\u00c5 resolution and belonged to the monoclinic space group <jats:italic>P<\/jats:italic>2<jats:sub>1<\/jats:sub> with one molecule of <jats:italic>Bm<\/jats:italic>PGA in the asymmetric unit.<\/jats:p>","DOI":"10.1107\/s1744309111040462","type":"journal-article","created":{"date-parts":[[2011,11,25]],"date-time":"2011-11-25T16:42:30Z","timestamp":1322239350000},"page":"1570-1574","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":6,"title":["Improved X-ray diffraction from <i>Bacillus megaterium<\/i> penicillin G acylase crystals through long cryosoaking dehydration"],"prefix":"10.1107","volume":"67","author":[{"given":"Catleya","family":"Rojviriya","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thunyaluck","family":"Pratumrat","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark A.","family":"Saper","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jirundon","family":"Yuvaniyama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"329","published-online":{"date-parts":[[2011,11,26]]},"reference":[{"key":"rp5063_bb1","doi-asserted-by":"crossref","first-page":"1413","DOI":"10.1107\/S0907444904013678","volume":"60","author":"Abergel","year":"2004","journal-title":"Acta Cryst. 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