{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T03:05:42Z","timestamp":1769051142456,"version":"3.49.0"},"reference-count":19,"publisher":"International Union of Crystallography (IUCr)","issue":"2","license":[{"start":{"date-parts":[[2012,1,25]],"date-time":"2012-01-25T00:00:00Z","timestamp":1327449600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},{"start":{"date-parts":[[2012,1,25]],"date-time":"2012-01-25T00:00:00Z","timestamp":1327449600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html#TDM"}],"content-domain":{"domain":["iucr.org","wiley.com","iucrj.org"],"crossmark-restriction":true},"short-container-title":["Acta Crystallogr F Struct Biol Cryst Commun","Acta Cryst F","Acta Cryst Sect F","Acta Crystallogr F","Acta Crystallogr Sect F","Acta Cryst F Struct Biol Cryst Commun","Acta Cryst Sect F Struct Biol Cryst Commun"],"published-print":{"date-parts":[[2012,2,1]]},"abstract":"<jats:p>DNA ligases join single-strand breaks in double-stranded DNA by catalyzing the formation of a phosphodiester bond between adjacent 5\u2032-phosphate and 3\u2032-hydroxyl termini. Their function is essential to maintain the integrity of the genome in DNA replication, recombination and repair. A recombinant ATP-dependent DNA ligase from the hyperthermophilic anaerobic archaeon<jats:italic>Thermococcus sibiricus<\/jats:italic>was expressed in<jats:italic>Escherichia coli<\/jats:italic>and purified. Crystals were grown by vapour diffusion using the hanging-drop method with 17%(<jats:italic>w<\/jats:italic>\/<jats:italic>v<\/jats:italic>) PEG 4000 and 8.5%(<jats:italic>v<\/jats:italic>\/<jats:italic>v<\/jats:italic>) 2-propanol as precipitants. A diffraction experiment was performed with a single crystal, which diffracted X-rays to 3.0\u2005\u00c5 resolution. The crystal belonged to space group<jats:italic>P<\/jats:italic>2<jats:sub>1<\/jats:sub>2<jats:sub>1<\/jats:sub>2<jats:sub>1<\/jats:sub>, with unit-cell parameters<jats:italic>a<\/jats:italic>\u00a0=\u00a058.590,<jats:italic>b<\/jats:italic>= 87.540,<jats:italic>c<\/jats:italic>= 126.300\u2005\u00c5.<\/jats:p>","DOI":"10.1107\/s1744309111050913","type":"journal-article","created":{"date-parts":[[2012,1,24]],"date-time":"2012-01-24T17:42:28Z","timestamp":1327426948000},"page":"163-165","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":9,"title":["Expression, purification, crystallization and preliminary crystallographic analysis of a thermostable DNA ligase from the archaeon<i>Thermococcus sibiricus<\/i>"],"prefix":"10.1107","volume":"68","author":[{"given":"T. E.","family":"Petrova","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E. 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