{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T01:42:43Z","timestamp":1790905363085,"version":"4.1.0"},"reference-count":21,"publisher":"International Union of Crystallography (IUCr)","issue":"2","license":[{"start":{"date-parts":[[2012,1,27]],"date-time":"2012-01-27T00:00:00Z","timestamp":1327622400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},{"start":{"date-parts":[[2012,1,27]],"date-time":"2012-01-27T00:00:00Z","timestamp":1327622400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html#TDM"}],"content-domain":{"domain":["iucr.org","wiley.com","iucrj.org"],"crossmark-restriction":true},"short-container-title":["Acta Crystallogr F Struct Biol Cryst Commun","Acta Cryst F","Acta Cryst Sect F","Acta Crystallogr F","Acta Crystallogr Sect F","Acta Cryst F Struct Biol Cryst Commun","Acta Cryst Sect F Struct Biol Cryst Commun"],"published-print":{"date-parts":[[2012,2,1]]},"abstract":"<jats:p>\n                    FrpB is an integral outer membrane protein from the human pathogen\n                    <jats:italic>Neisseria meningitidis<\/jats:italic>\n                    . It is a member of the TonB-dependent transporter family and promotes the uptake of iron across the outer membrane. There is also evidence that FrpB is an antigen and hence a potential component of a vaccine against meningococcal meningitis. FrpB incorporating a polyhistidine tag was overexpressed in\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    into inclusion bodies. The protein was then solubilized in urea, refolded and purified to homogeneity. Two separate antigenic variants of FrpB were crystallized by sitting-drop vapour diffusion. Crystals of the F5-1 variant diffracted to 2.4\u2005\u00c5 resolution and belonged to space group\n                    <jats:italic>C<\/jats:italic>\n                    2, with unit-cell parameters\n                    <jats:italic>a<\/jats:italic>\n                    = 176.5,\n                    <jats:italic>b<\/jats:italic>\n                    = 79.4,\n                    <jats:italic>c<\/jats:italic>\n                    = 75.9\u2005\u00c5, \u03b2 = 98.3\u00b0. Crystal-packing calculations suggested the presence of a monomer in the asymmetric unit. Crystals of the F3-3 variant also diffracted to 2.4\u2005\u00c5 resolution and belonged to space group\n                    <jats:italic>P<\/jats:italic>\n                    2\n                    <jats:sub>1<\/jats:sub>\n                    2\n                    <jats:sub>1<\/jats:sub>\n                    2\n                    <jats:sub>1<\/jats:sub>\n                    , with unit-cell parameters\n                    <jats:italic>a<\/jats:italic>\n                    = 85.3,\n                    <jats:italic>b<\/jats:italic>\n                    \u00a0=\u00a0104.6,\n                    <jats:italic>c<\/jats:italic>\n                    = 269.1\u2005\u00c5. Preliminary analysis suggested the presence of an FrpB trimer in the asymmetric unit.\n                  <\/jats:p>","DOI":"10.1107\/s1744309111056028","type":"journal-article","created":{"date-parts":[[2012,1,26]],"date-time":"2012-01-26T11:04:33Z","timestamp":1327575873000},"page":"231-235","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":11,"title":["Refolding, purification and crystallization of the FrpB outer membrane iron transporter from\n                    <i>Neisseria meningitidis<\/i>"],"prefix":"10.1107","volume":"68","author":[{"given":"Muhammad","family":"Saleem","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen M.","family":"Prince","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hema","family":"Patel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hannah","family":"Chan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ian M.","family":"Feavers","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeremy P.","family":"Derrick","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"329","published-online":{"date-parts":[[2012,1,27]]},"reference":[{"key":"rl5016_bb1","doi-asserted-by":"crossref","first-page":"2041","DOI":"10.1128\/jb.177.8.2041-2049.1995","volume":"177","author":"Beucher","year":"1995","journal-title":"J. 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