{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T11:59:49Z","timestamp":1774526389812,"version":"3.50.1"},"reference-count":18,"publisher":"International Union of Crystallography (IUCr)","issue":"11","license":[{"start":{"date-parts":[[2012,10,30]],"date-time":"2012-10-30T00:00:00Z","timestamp":1351555200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},{"start":{"date-parts":[[2012,10,30]],"date-time":"2012-10-30T00:00:00Z","timestamp":1351555200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html#TDM"}],"content-domain":{"domain":["iucr.org","wiley.com","iucrj.org"],"crossmark-restriction":true},"short-container-title":["Acta Crystallogr F Struct Biol Cryst Commun","Acta Cryst F","Acta Cryst Sect F","Acta Crystallogr F","Acta Crystallogr Sect F","Acta Cryst F Struct Biol Cryst Commun","Acta Cryst Sect F Struct Biol Cryst Commun"],"published-print":{"date-parts":[[2012,11,1]]},"abstract":"<jats:p>Phospholipases A<jats:sub>2<\/jats:sub>(PLA<jats:sub>2<\/jats:sub>s) are the major component of snake venoms and exert a variety of relevant toxic actions such as neurotoxicity and myotoxicity, amongst others. An acidic PLA<jats:sub>2<\/jats:sub>, here named AhV_aPA, was purified from<jats:italic>Agkistrodon halys pallas<\/jats:italic>venom by means of a three-step chromatographic procedure. AhV_aPA migrated as a single band on SDS\u2013PAGE gels, with a molecular weight of about 14\u2005kDa. Like other acidic aPLA<jats:sub>2<\/jats:sub>s, AhV_aPA has high enzymatic activity. Tension measurements of mouse thoracic aortic rings remarkably indicated that AhV_aPA could induce a further contractile response on the 60\u2005m<jats:italic>M<\/jats:italic>K<jats:sup>+<\/jats:sup>-induced contraction, with an EC<jats:sub>50<\/jats:sub>of 369\u2005nmol\u2005l<jats:sup>\u22121<\/jats:sup>. Rod-shaped crystals were obtained by the hanging-drop vapour-diffusion method and diffracted to a resolution limit of 2.30\u2005\u00c5. The crystals belonged to space group<jats:italic>P<\/jats:italic>222, with unit-cell parameters<jats:italic>a<\/jats:italic>= 44.27,<jats:italic>b<\/jats:italic>= 68.39,<jats:italic>c<\/jats:italic>= 81.54\u2005\u00c5.<\/jats:p>","DOI":"10.1107\/s1744309112038523","type":"journal-article","created":{"date-parts":[[2012,10,29]],"date-time":"2012-10-29T19:30:47Z","timestamp":1351539047000},"page":"1329-1332","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":4,"title":["Purification, crystallization and preliminary X-ray diffraction analysis of an acidic phospholipase A<sub>2<\/sub>with vasoconstrictor activity from<i>Agkistrodon halys pallas<\/i>venom"],"prefix":"10.1107","volume":"68","author":[{"given":"Zhisong","family":"Zou","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fuxing","family":"Zeng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lu","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liwen","family":"Niu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maikun","family":"Teng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xu","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"329","published-online":{"date-parts":[[2012,10,30]]},"reference":[{"key":"dp5033_bb14","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1002\/prot.22858","volume":"79","author":"dos Santos","year":"2011","journal-title":"Proteins"},{"key":"dp5033_bb1","doi-asserted-by":"crossref","first-page":"25428","DOI":"10.1074\/jbc.M804146200","volume":"283","author":"Duncan","year":"2008","journal-title":"J. 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