{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T02:29:37Z","timestamp":1790994577029,"version":"4.1.0"},"reference-count":44,"publisher":"International Union of Crystallography (IUCr)","issue":"3","license":[{"start":{"date-parts":[[2024,4,24]],"date-time":"2024-04-24T00:00:00Z","timestamp":1713916800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/legalcode"},{"start":{"date-parts":[[2024,4,24]],"date-time":"2024-04-24T00:00:00Z","timestamp":1713916800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/legalcode"}],"funder":[{"DOI":"10.13039\/501100018833","name":"Agence de l'innovation de D\u00e9fense","doi-asserted-by":"publisher","award":["ASTR-0010-01"],"award-info":[{"award-number":["ASTR-0010-01"]}],"id":[{"id":"10.13039\/501100018833","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["PaNuVi"],"award-info":[{"award-number":["PaNuVi"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002915","name":"Fondation pour la Recherche M\u00e9dicale","doi-asserted-by":"publisher","award":["DCM20181039531"],"award-info":[{"award-number":["DCM20181039531"]}],"id":[{"id":"10.13039\/501100002915","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007356","name":"Fondation M\u00e9diterran\u00e9e Infection","doi-asserted-by":"publisher","award":["Infectiopole Sud"],"award-info":[{"award-number":["Infectiopole Sud"]}],"id":[{"id":"10.13039\/100007356","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011658","name":"French Infrastructure for Integrated Structural Biology","doi-asserted-by":"publisher","award":["ANR-10-INBS-0005"],"award-info":[{"award-number":["ANR-10-INBS-0005"]}],"id":[{"id":"10.13039\/501100011658","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004793","name":"Minist\u00e8re de l'Education Nationale, de l'Enseignement Sup\u00e9rieur et de la Recherche","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004793","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["iucr.org","wiley.com","iucrj.org"],"crossmark-restriction":true},"short-container-title":["IUCrJ","IUCr J","Int Union Crystallogr J","Int Union Cryst J"],"accepted":{"date-parts":[[2024,4,8]]},"published-print":{"date-parts":[[2024,5,1]]},"abstract":"<jats:p>The large <jats:italic>Bunyavirales<\/jats:italic> order includes several families of viruses with a segmented ambisense (\u2212) RNA genome and a cytoplasmic life cycle that starts by synthesizing viral mRNA. The initiation of transcription, which is common to all members, relies on an endonuclease activity that is responsible for cap-snatching. In La Crosse virus, an orthobunyavirus, it has previously been shown that the cap-snatching endonuclease resides in the N-terminal domain of the L protein. Orthobunyaviruses are transmitted by arthropods and cause diseases in cattle. However, California encephalitis virus, La Crosse virus and Jamestown Canyon virus are North American species that can cause encephalitis in humans. No vaccines or antiviral drugs are available. In this study, three known Influenza virus endonuclease inhibitors (DPBA, L-742,001 and baloxavir) were repurposed on the La Crosse virus endonuclease. Their inhibition was evaluated by fluorescence resonance energy transfer and their mode of binding was then assessed by differential scanning fluorimetry and microscale thermophoresis. Finally, two crystallographic structures were obtained in complex with L-742,001 and baloxavir, providing access to the structural determinants of inhibition and offering key information for the further development of <jats:italic>Bunyavirales<\/jats:italic> endonuclease inhibitors.<\/jats:p>","DOI":"10.1107\/s205225252400304x","type":"journal-article","created":{"date-parts":[[2024,4,24]],"date-time":"2024-04-24T15:26:54Z","timestamp":1713972414000},"page":"374-383","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":8,"title":["Biophysical and structural study of La Crosse virus endonuclease inhibition for the development of new antiviral options"],"prefix":"10.1107","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5636-8208","authenticated-orcid":true,"given":"Mikael","family":"Feracci","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergio","family":"Hernandez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5627-2310","authenticated-orcid":true,"given":"Laura","family":"Garlatti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clemence","family":"Mondielli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9667-0196","authenticated-orcid":true,"given":"Renaud","family":"Vincentelli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4924-1991","authenticated-orcid":true,"given":"Bruno","family":"Canard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Reguera","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8351-4992","authenticated-orcid":true,"given":"Fran\u00e7ois","family":"Ferron","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6108-6966","authenticated-orcid":true,"given":"Karine","family":"Alvarez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"329","published-online":{"date-parts":[[2024,4,24]]},"reference":[{"key":"lz5070_bb43","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1107\/S2059798323003595","volume":"79","author":"Agirre","year":"2023","journal-title":"Acta Cryst. D"},{"key":"lz5070_bb1","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1107\/S2059798320003198","volume":"76","author":"Beilsten-Edmands","year":"2020","journal-title":"Acta Cryst. D"},{"key":"lz5070_bb2","doi-asserted-by":"crossref","first-page":"e1114","DOI":"10.1093\/cid\/ciac403","volume":"76","author":"Boutzoukas","year":"2023","journal-title":"Clin. Infect. Dis."},{"key":"lz5070_bb3","doi-asserted-by":"crossref","first-page":"9096","DOI":"10.1128\/JVI.00995-10","volume":"84","author":"Cr\u00e9pin","year":"2010","journal-title":"J. Virol."},{"key":"lz5070_bb4","doi-asserted-by":"crossref","first-page":"e1002830","DOI":"10.1371\/journal.ppat.1002830","volume":"8","author":"DuBois","year":"2012","journal-title":"PLoS Pathog."},{"key":"lz5070_bb5","doi-asserted-by":"crossref","first-page":"486","DOI":"10.1107\/S0907444910007493","volume":"66","author":"Emsley","year":"2010","journal-title":"Acta Cryst. D"},{"key":"lz5070_bb6","doi-asserted-by":"crossref","first-page":"16424","DOI":"10.1038\/s41598-021-95757-2","volume":"11","author":"Evans","year":"2021","journal-title":"Sci. Rep."},{"key":"lz5070_bb7","doi-asserted-by":"crossref","first-page":"104947","DOI":"10.1016\/j.antiviral.2020.104947","volume":"183","author":"Fern\u00e1ndez-Garc\u00eda","year":"2020","journal-title":"Antiviral Res."},{"key":"lz5070_bb8","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.antiviral.2012.07.007","volume":"96","author":"Ferron","year":"2012","journal-title":"Antiviral Res."},{"key":"lz5070_bb9","doi-asserted-by":"crossref","first-page":"1304","DOI":"10.1128\/AAC.40.5.1304","volume":"40","author":"Hastings","year":"1996","journal-title":"Antimicrob. Agents Chemother."},{"key":"lz5070_bb10","doi-asserted-by":"crossref","first-page":"e2039","DOI":"10.1002\/rmv.2039","volume":"29","author":"Horst","year":"2019","journal-title":"Rev. Med. Virol."},{"key":"lz5070_bb11","doi-asserted-by":"crossref","first-page":"5504","DOI":"10.1128\/AAC.00888-16","volume":"60","author":"Jones","year":"2016","journal-title":"Antimicrob. Agents Chemother."},{"key":"lz5070_bb12","doi-asserted-by":"crossref","first-page":"10914","DOI":"10.1093\/nar\/gkz838","volume":"47","author":"Jones","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"lz5070_bb13","doi-asserted-by":"crossref","first-page":"e1002831","DOI":"10.1371\/journal.ppat.1002831","volume":"8","author":"Kowalinski","year":"2012","journal-title":"PLoS Pathog."},{"key":"lz5070_bb14","first-page":"e02173","volume":"96","author":"Kuang","year":"2022","journal-title":"J. Virol."},{"key":"lz5070_bb16","doi-asserted-by":"crossref","first-page":"2778","DOI":"10.1021\/ci200227u","volume":"51","author":"Laskowski","year":"2011","journal-title":"J. Chem. Inf. Model."},{"key":"lz5070_bb17","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1186\/s12985-017-0725-5","volume":"14","author":"Lwande","year":"2017","journal-title":"Virol. J."},{"key":"lz5070_bb18","doi-asserted-by":"crossref","first-page":"658","DOI":"10.1107\/S0021889807021206","volume":"40","author":"McCoy","year":"2007","journal-title":"J. Appl. Cryst."},{"key":"lz5070_bb19","doi-asserted-by":"crossref","first-page":"e2200481119","DOI":"10.1073\/pnas.2200481119","volume":"119","author":"McDermott","year":"2022","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"lz5070_bb20","unstructured":"Mondielli, C. (2018). Th\u00e9se de doctorat, Universit\u00e9 Aix-Marseille, France. https:\/\/theses.fr\/api\/v1\/document\/2018AIXM0385."},{"key":"lz5070_bb21","doi-asserted-by":"crossref","first-page":"e1001038","DOI":"10.1371\/journal.ppat.1001038","volume":"6","author":"Morin","year":"2010","journal-title":"PLoS Pathog."},{"key":"lz5070_bb22","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1107\/S0907444911001314","volume":"67","author":"Murshudov","year":"2011","journal-title":"Acta Cryst. D"},{"key":"lz5070_bb23","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.antiviral.2018.10.008","volume":"160","author":"Noshi","year":"2018","journal-title":"Antiviral Res."},{"key":"lz5070_bb24","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.coviro.2019.01.006","volume":"35","author":"O'Hanlon","year":"2019","journal-title":"Curr. Opin. Virol."},{"key":"lz5070_bb25","doi-asserted-by":"crossref","first-page":"9633","DOI":"10.1038\/s41598-018-27890-4","volume":"8","author":"Omoto","year":"2018","journal-title":"Sci. Rep."},{"key":"lz5070_bb26","doi-asserted-by":"crossref","first-page":"1701","DOI":"10.1093\/cid\/ciaa308","volume":"72","author":"P\u00e9rot","year":"2021","journal-title":"Clin. Infect. Dis."},{"key":"lz5070_bb27","doi-asserted-by":"crossref","first-page":"e1005636","DOI":"10.1371\/journal.ppat.1005636","volume":"12","author":"Reguera","year":"2016","journal-title":"PLoS Pathog."},{"key":"lz5070_bb28","doi-asserted-by":"crossref","first-page":"e1001101","DOI":"10.1371\/journal.ppat.1001101","volume":"6","author":"Reguera","year":"2010","journal-title":"PLoS Pathog."},{"key":"lz5070_bb29","doi-asserted-by":"crossref","first-page":"1287","DOI":"10.3201\/eid2606.191431","volume":"26","author":"Rodriguez","year":"2020","journal-title":"Emerg. Infect. Dis."},{"key":"lz5070_bb30","doi-asserted-by":"crossref","first-page":"3063","DOI":"10.1016\/j.ccr.2012.07.006","volume":"256","author":"Rogolino","year":"2012","journal-title":"Coord. Chem. Rev."},{"key":"lz5070_bb32","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1107\/S2052252518001021","volume":"5","author":"Saez-Ayala","year":"2018","journal-title":"IUCrJ"},{"key":"lz5070_bb31","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/j.antiviral.2018.12.008","volume":"162","author":"Saez-Ayala","year":"2019","journal-title":"Antiviral Res."},{"key":"lz5070_bb33","doi-asserted-by":"crossref","first-page":"3669","DOI":"10.1073\/pnas.1519772113","volume":"113","author":"Song","year":"2016","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"lz5070_bb34","doi-asserted-by":"crossref","first-page":"6498","DOI":"10.1073\/pnas.90.14.6498","volume":"90","author":"Steitz","year":"1993","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"lz5070_bb36","doi-asserted-by":"crossref","first-page":"100486","DOI":"10.1016\/j.jbc.2021.100486","volume":"296","author":"Todd","year":"2021","journal-title":"J. Biol. Chem."},{"key":"lz5070_bb37","doi-asserted-by":"crossref","first-page":"2827","DOI":"10.1128\/AAC.38.12.2827","volume":"38","author":"Tomassini","year":"1994","journal-title":"Antimicrob Agents Chemother"},{"key":"lz5070_bb38","doi-asserted-by":"crossref","first-page":"807","DOI":"10.4269\/ajtmh.21-0294","volume":"105","author":"Vahey","year":"2021","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"lz5070_bb39","doi-asserted-by":"crossref","first-page":"1769","DOI":"10.1099\/0022-1317-77-8-1769","volume":"77","author":"Vapalahti","year":"1996","journal-title":"J. Gen. Virol."},{"key":"lz5070_bb40","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1093\/protein\/8.2.127","volume":"8","author":"Wallace","year":"1995","journal-title":"Protein Eng. Des. Sel."},{"key":"lz5070_bb41","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1186\/s42522-022-00069-8","volume":"4","author":"Wernike","year":"2022","journal-title":"One Health Outlook"},{"key":"lz5070_bb42","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/bs.aivir.2017.07.001","volume":"99","author":"Wernike","year":"2017","journal-title":"Adv. Virus Res."},{"key":"lz5070_bb44","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1107\/S0021889809045701","volume":"43","author":"Winter","year":"2010","journal-title":"J. Appl. Cryst."},{"key":"lz5070_bb45","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1107\/S2059798317017235","volume":"74","author":"Winter","year":"2018","journal-title":"Acta Cryst. D"},{"key":"lz5070_bb46","doi-asserted-by":"crossref","first-page":"168","DOI":"10.3389\/fvets.2020.00168","volume":"7","author":"Yanase","year":"2020","journal-title":"Front. Vet. Sci."}],"container-title":["IUCrJ"],"original-title":[],"link":[{"URL":"https:\/\/journals.iucr.org\/m\/issues\/2024\/03\/00\/lz5070\/lz5070.pdf","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.iucr.org\/m\/issues\/2024\/03\/00\/lz5070\/lz5070.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,13]],"date-time":"2025-01-13T11:32:43Z","timestamp":1736767963000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.iucr.org\/paper?S205225252400304X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,4,24]]},"references-count":44,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2024,5]]}},"alternative-id":["S205225252400304X"],"URL":"https:\/\/doi.org\/10.1107\/s205225252400304x","relation":{"is-supplemented-by":[{"id-type":"doi","id":"10.2210\/pdb7oa4\/pdb","asserted-by":"subject"},{"id-type":"doi","id":"10.2210\/pdb7plr\/pdb","asserted-by":"subject"}]},"ISSN":["2052-2525"],"issn-type":[{"value":"2052-2525","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,4,24]]},"assertion":[{"value":"IUCrJ","order":0,"name":"journal","label":"Publication","explanation":{"URL":"https:\/\/www.iucrj.org\/"},"group":{"name":"general","label":"General"}},{"value":"research papers","order":1,"name":"content_type","label":"Content type","explanation":{"URL":"https:\/\/journals.iucr.org\/m\/services\/notesforauthors.html"},"group":{"name":"general","label":"General"}},{"value":"Available","URL":"https:\/\/scripts.iucr.org\/cgi-bin\/citedin?lz5070","order":2,"name":"article_metrics","label":"Article metrics","group":{"name":"general","label":"General"}},{"value":"Yes","order":0,"name":"peer_reviewed","label":"Peer reviewed","group":{"name":"peer_review","label":"Peer review"}},{"value":"Single blind","order":1,"name":"review_process","label":"Review process","group":{"name":"peer_review","label":"Peer review"}},{"value":"13 December 2023","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication history"}},{"value":"8 April 2024","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication history"}},{"value":"24 April 2024","order":2,"name":"published_online","label":"Published online","group":{"name":"publication_history","label":"Publication history"}},{"value":"This article has supporting information","name":"supplementary_materials","label":"Supplementary materials","explanation":{"URL":"https:\/\/scripts.iucr.org\/cgi-bin\/sendsup?lz5070"}},{"value":"\u00a9 2024 Mikael Feracci et al.","order":0,"name":"copyright","label":"Copyright","explanation":{"URL":"https:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},"group":{"name":"copyright_and_licencing","label":"Copyright and licencing"}},{"value":"Creative Commons Attribution (CC-BY)","order":1,"name":"license","label":"Licence","explanation":{"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/legalcode"},"group":{"name":"copyright_and_licencing","label":"Copyright and licencing"}}]}}