{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T06:56:05Z","timestamp":1791356165828,"version":"4.3.1"},"reference-count":35,"publisher":"International Union of Crystallography (IUCr)","issue":"5","license":[{"start":{"date-parts":[[2024,7,4]],"date-time":"2024-07-04T00:00:00Z","timestamp":1720051200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/legalcode"},{"start":{"date-parts":[[2024,7,4]],"date-time":"2024-07-04T00:00:00Z","timestamp":1720051200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/legalcode"}],"content-domain":{"domain":["iucr.org","wiley.com","iucrj.org"],"crossmark-restriction":true},"short-container-title":["IUCrJ","IUCr J","Int Union Crystallogr J","Int Union Cryst J"],"accepted":{"date-parts":[[2024,6,10]]},"published-print":{"date-parts":[[2024,9,1]]},"abstract":"<jats:p>\n                    Single-particle cryo-electron microscopy (cryo-EM) has become an essential structural determination technique with recent hardware developments making it possible to reach atomic resolution, at which individual atoms, including hydrogen atoms, can be resolved. In this study, we used the enzyme involved in the penultimate step of riboflavin biosynthesis as a test specimen to benchmark a recently installed microscope and determine if other protein complexes could reach a resolution of 1.5\u2005\u00c5 or better, which so far has only been achieved for the iron carrier ferritin. Using state-of-the-art microscope and detector hardware as well as the latest software techniques to overcome microscope and sample limitations, a 1.42\u2005\u00c5 map of\n                    <jats:italic>Aquifex aeolicus<\/jats:italic>\n                    lumazine synthase (AaLS) was obtained from a 48\u2005h microscope session. In addition to water molecules and ligands involved in the function of AaLS, we can observe positive density for \u223c50% of the hydrogen atoms. A small improvement in the resolution was achieved by Ewald sphere correction which was expected to limit the resolution to \u223c1.5\u2005\u00c5 for a molecule of this diameter. Our study confirms that other protein complexes can be solved to near-atomic resolution. Future improvements in specimen preparation and protein complex stabilization may allow more flexible macromolecules to reach this level of resolution and should become a priority of study in the field.\n                  <\/jats:p>","DOI":"10.1107\/s2052252524005530","type":"journal-article","created":{"date-parts":[[2024,6,26]],"date-time":"2024-06-26T06:11:17Z","timestamp":1719382277000},"page":"723-729","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":3,"title":["Structure of\n                    <i>Aquifex aeolicus<\/i>\n                    lumazine synthase by cryo-electron microscopy to 1.42\u2005\u00c5 resolution"],"prefix":"10.1107","volume":"11","author":[{"given":"Christos G.","family":"Savva","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01q3tbs38","id-type":"ROR","asserted-by":"publisher"}],"department":["Biological and Environmental Science and Engineering"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed A.","family":"Sobhy","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01q3tbs38","id-type":"ROR","asserted-by":"publisher"}],"department":["Biological and Environmental Science and Engineering"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alfredo","family":"De Biasio","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01q3tbs38","id-type":"ROR","asserted-by":"publisher"}],"department":["Biological and Environmental Science and Engineering"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samir M.","family":"Hamdan","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01q3tbs38","id-type":"ROR","asserted-by":"publisher"}],"department":["Biological and Environmental Science and Engineering"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"329","published-online":{"date-parts":[[2024,7,4]]},"reference":[{"key":"eh5018_bb1","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1107\/S2059798323003595","volume":"79","author":"Agirre","year":"2023","journal-title":"Acta Cryst. 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