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Recent investigations underscore the involvement of a small EDRK-rich factor 1a (SERF1a) in promoting Httex1 fibrillization through interactions with its N terminus. By establishing an integrated approach with size-exclusion-column-based small- and wide-angle X-ray scattering (SEC-SWAXS), NMR, and molecular simulations using\n                    <jats:italic>Rosetta<\/jats:italic>\n                    , the analysis here reveals a tight binding of two NT17 fragments of Httex1 (comprising the initial 17 amino acids at the N terminus) to the N-terminal region of SERF1a. In contrast, examination of the complex structure of SERF1a with a coiled NT17-polyQ peptide (33 amino acids in total) indicates sparse contacts of the NT17 and polyQ segments with the N-terminal side of SERF1a. Furthermore, the integrated SEC-SWAXS and molecular-simulation analysis suggests that the coiled NT17 segment can transform into a helical conformation when associated with a polyQ segment exhibiting high helical content. Intriguingly, NT17-polyQ peptides with enhanced secondary structures display diminished interactions with SERF1a. This insight into the conformation-dependent binding of NT17 provides clues to a catalytic association mechanism underlying SERF1a's facilitation of Httext1 fibrillization.\n                  <\/jats:p>","DOI":"10.1107\/s2052252524006341","type":"journal-article","created":{"date-parts":[[2024,8,1]],"date-time":"2024-08-01T12:52:56Z","timestamp":1722516776000},"page":"849-858","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":6,"title":["Binding structures of SERF1a with NT17-polyQ peptides of huntingtin exon 1 revealed by SEC-SWAXS, NMR and molecular simulation"],"prefix":"10.1107","volume":"11","author":[{"given":"Tien-Chang","family":"Lin","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00zdnkx70","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Chemical Engineering"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0610-6856","authenticated-orcid":true,"given":"Orion","family":"Shih","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00k575643","id-type":"ROR","asserted-by":"publisher"}]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tien-Ying","family":"Tsai","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05bxb3784","id-type":"ROR","asserted-by":"publisher"}],"department":["Genomics Research Center"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2387-3600","authenticated-orcid":true,"given":"Yi-Qi","family":"Yeh","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00k575643","id-type":"ROR","asserted-by":"publisher"}]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kuei-Fen","family":"Liao","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00k575643","id-type":"ROR","asserted-by":"publisher"}]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4484-2093","authenticated-orcid":true,"given":"Bradley W.","family":"Mansel","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00k575643","id-type":"ROR","asserted-by":"publisher"}]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying-Jen","family":"Shiu","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00k575643","id-type":"ROR","asserted-by":"publisher"}]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2961-0748","authenticated-orcid":true,"given":"Chi-Fon","family":"Chang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05bxb3784","id-type":"ROR","asserted-by":"publisher"}],"department":["Genomics Research Center"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"An-Chung","family":"Su","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00zdnkx70","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Chemical Engineering"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun-Ru","family":"Chen","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05bxb3784","id-type":"ROR","asserted-by":"publisher"}],"department":["Genomics Research Center"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2247-5061","authenticated-orcid":true,"given":"U-Ser","family":"Jeng","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00zdnkx70","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Chemical Engineering"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"329","published-online":{"date-parts":[[2024,8,8]]},"reference":[{"key":"ti5031_bb1","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1515\/bmc-2015-0001","volume":"6","author":"Arndt","year":"2015","journal-title":"Biomol. 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