{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T14:48:28Z","timestamp":1785854908475,"version":"3.56.0"},"reference-count":73,"publisher":"International Union of Crystallography (IUCr)","issue":"4","license":[{"start":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T00:00:00Z","timestamp":1780617600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/legalcode"},{"start":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T00:00:00Z","timestamp":1780617600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/legalcode"}],"funder":[{"name":"Royal Society Wolfson Fellowship","award":["RSWF\\R2\\182017"],"award-info":[{"award-number":["RSWF\\R2\\182017"]}]},{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["No. 210734\/Z\/18\/Z"],"award-info":[{"award-number":["No. 210734\/Z\/18\/Z"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["227298\/Z\/23\/Z"],"award-info":[{"award-number":["227298\/Z\/23\/Z"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000265","name":"Medical Research Council","doi-asserted-by":"publisher","award":["UKRI330"],"award-info":[{"award-number":["UKRI330"]}],"id":[{"id":"10.13039\/501100000265","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/T008741\/1"],"award-info":[{"award-number":["BB\/T008741\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/T008784\/1"],"award-info":[{"award-number":["BB\/T008784\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009187","name":"Medical Research Foundation","doi-asserted-by":"publisher","award":["MRF-145-0004-TPG-AVISO"],"award-info":[{"award-number":["MRF-145-0004-TPG-AVISO"]}],"id":[{"id":"10.13039\/501100009187","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009187","name":"Medical Research Foundation","doi-asserted-by":"publisher","award":["BB\/T008784\/1"],"award-info":[{"award-number":["BB\/T008784\/1"]}],"id":[{"id":"10.13039\/501100009187","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000265","name":"Medical Research Council","doi-asserted-by":"publisher","award":["MR\/T016035\/1"],"award-info":[{"award-number":["MR\/T016035\/1"]}],"id":[{"id":"10.13039\/501100000265","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010663","name":"H2020 European Research Council","doi-asserted-by":"publisher","award":["101021207"],"award-info":[{"award-number":["101021207"]}],"id":[{"id":"10.13039\/100010663","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["iucr.org","wiley.com","iucrj.org"],"crossmark-restriction":true},"short-container-title":["IUCrJ","IUCr J","Int Union Crystallogr J","Int Union Cryst J"],"accepted":{"date-parts":[[2026,4,2]]},"published-print":{"date-parts":[[2026,7,1]]},"abstract":"<jats:p>\n                    We describe the design and implementation of a drop-on-fixed-target method for time-resolved serial crystallography at both synchrotron and XFEL facilities. A piezoelectric droplet dispensing pipette is employed for addition of picolitre volume aqueous droplets (\u223c40\u201390\u2005pl; \u223c40\u201355\u2005\u00b5m diameter sphere), containing (co-)substrate(s) or ligand(s), onto enzyme microcrystals previously loaded into the trapezoidal wells of an etched crystalline silicon fixed-target chip containing 25\u2005600 wells in a high-density, square grid with 125\u2005\u00b5m centre-to-centre well spacing. These features demand exquisite accuracy and thereby constrain motion controls to enable robust time-resolved crystallographic studies. The system was tested with three enzyme systems, comprising lysozyme and two \u03b2-lactamases, CTX-M-15 and AmpC\n                    <jats:sub>EC<\/jats:sub>\n                    . Mitigation strategies for cross-well contamination, including the implementation of interleaved controls, are described; the overall performance of the system at synchrotron and X-ray free-electron laser facilities was evaluated. This drop-on-fixed-target method is a reliable framework for time-resolved crystallography and will improve the consistency of measurements across facilities.\n                  <\/jats:p>","DOI":"10.1107\/s2052252526003489","type":"journal-article","created":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T14:52:02Z","timestamp":1779202322000},"page":"471-484","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":0,"title":["Drop-on-fixed-target reaction initiation approach for serial and time-resolved crystallography"],"prefix":"10.1107","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8646-1796","authenticated-orcid":true,"given":"Jos J. A. G.","family":"Kamps","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03gq8fr08","id-type":"ROR","asserted-by":"publisher"}],"department":["Research Complex at Harwell"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8611-4743","authenticated-orcid":true,"given":"Philip","family":"Hinchliffe","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0524sp257","id-type":"ROR","asserted-by":"publisher"}],"department":["School of Biochemistry and Cellular and Molecular Medicine"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-3040-3817","authenticated-orcid":true,"given":"Johan","family":"Glerup","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1049-7674","authenticated-orcid":true,"given":"Emily I.","family":"Freeman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3187-1469","authenticated-orcid":true,"given":"Pauline A.","family":"Lang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/052gg0110","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Chemistry and the Ineos Oxford Institute for Antimicrobial Research"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2180-3235","authenticated-orcid":true,"given":"Catherine L.","family":"Tooke","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/002h8g185","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Life Sciences"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3879-3339","authenticated-orcid":true,"given":"Michael","family":"Beer","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0524sp257","id-type":"ROR","asserted-by":"publisher"}],"department":["School of Biochemistry and Cellular and Molecular Medicine"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-5083-5018","authenticated-orcid":true,"given":"Laura","family":"Parkinson","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0524sp257","id-type":"ROR","asserted-by":"publisher"}],"department":["School of Biochemistry and Cellular and Molecular Medicine"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8777-0080","authenticated-orcid":true,"given":"Do-Heon","family":"Gu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sehan","family":"Park","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04xysgw12","id-type":"ROR","asserted-by":"publisher"}],"department":["Pohang Accelerator Laboratory"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-3030-6180","authenticated-orcid":true,"given":"Nicholas","family":"Devenish","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6116-7483","authenticated-orcid":true,"given":"Tiankun","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5931-5790","authenticated-orcid":true,"given":"Anastasya","family":"Shilova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samanpreet","family":"Kaur","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/052gg0110","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Chemistry and the Ineos Oxford Institute for Antimicrobial Research"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3448-9559","authenticated-orcid":true,"given":"Patrick","family":"Rabe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0290-6565","authenticated-orcid":true,"given":"Christopher J.","family":"Schofield","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/052gg0110","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Chemistry and the Ineos Oxford Institute for Antimicrobial Research"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James","family":"Spencer","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0524sp257","id-type":"ROR","asserted-by":"publisher"}],"department":["School of Biochemistry and Cellular and Molecular Medicine"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9007-4892","authenticated-orcid":true,"given":"Jaehyun","family":"Park","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04xysgw12","id-type":"ROR","asserted-by":"publisher"}],"department":["Pohang Accelerator Laboratory"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2104-7057","authenticated-orcid":true,"given":"Robin L.","family":"Owen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7803-1777","authenticated-orcid":true,"given":"Allen M.","family":"Orville","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1793-7030","authenticated-orcid":true,"given":"Pierre","family":"Aller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"329","published-online":{"date-parts":[[2026,6,5]]},"reference":[{"key":"zf5029_bb1","doi-asserted-by":"crossref","first-page":"1948","DOI":"10.1107\/S0907444902016657","volume":"58","author":"Adams","year":"2002","journal-title":"Acta Cryst. D"},{"key":"zf5029_bb900","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1107\/S2059798323003595","volume":"79","author":"Agirre","year":"2023","journal-title":"Acta Cryst. D"},{"key":"zf5029_bb3","doi-asserted-by":"crossref","unstructured":"Aller, P. & Orville, A. M. (2021). Structural Proteomics. Methods in Molecular Biology, Vol. 2305, edited by R. J. Owens, ch. 11, pp. 203-228. Springer US.","DOI":"10.1007\/978-1-0716-1406-8_11"},{"key":"zf5029_bb4","first-page":"20251119689295","author":"Aller","year":"2025","journal-title":"bioRxiv"},{"key":"zf5029_bb5","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1016\/j.str.2018.10.027","volume":"27","author":"Apel","year":"2019","journal-title":"Structure"},{"key":"zf5029_bb6","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/bs.mie.2024.10.004","volume":"709","author":"Beilsten-Edmands","year":"2024","journal-title":"Methods Enzymol."},{"key":"zf5029_bb7","doi-asserted-by":"crossref","first-page":"769","DOI":"10.1107\/S2052252517013124","volume":"4","author":"Beyerlein","year":"2017","journal-title":"IUCrJ"},{"key":"zf5029_bb8","first-page":"20250504652045","author":"Brewster","year":"2025","journal-title":"bioRxiv"},{"key":"zf5029_bb9","doi-asserted-by":"crossref","first-page":"4461","DOI":"10.1038\/s41467-021-24757-7","volume":"12","author":"Butryn","year":"2021","journal-title":"Nat. Commun."},{"key":"zf5029_bb10","doi-asserted-by":"crossref","first-page":"7139","DOI":"10.1021\/acs.analchem.9b00311","volume":"91","author":"Calvey","year":"2019","journal-title":"Anal. Chem."},{"key":"zf5029_bb11","doi-asserted-by":"crossref","first-page":"678","DOI":"10.1107\/S2052252523007595","volume":"10","author":"Carrillo","year":"2023","journal-title":"IUCrJ"},{"key":"zf5029_bb100","doi-asserted-by":"crossref","unstructured":"Castanheira, M., Simner, P. J. & Bradford, P. A. (2021). JAC Antimicrobial Resistance, 3, dlab092.","DOI":"10.1093\/jacamr\/dlab092"},{"key":"zf5029_bb12","doi-asserted-by":"crossref","first-page":"25634","DOI":"10.1073\/pnas.1901864116","volume":"116","author":"Dasgupta","year":"2019","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"zf5029_bb13","doi-asserted-by":"crossref","first-page":"1820","DOI":"10.1107\/S1600577519009329","volume":"26","author":"Davy","year":"2019","journal-title":"J. Synchrotron Rad."},{"key":"zf5029_bb14","doi-asserted-by":"crossref","first-page":"1000","DOI":"10.1107\/S2059798318011634","volume":"74","author":"Doak","year":"2018","journal-title":"Acta Cryst. D"},{"key":"zf5029_bb15","doi-asserted-by":"crossref","first-page":"1725","DOI":"10.1107\/S1600576724008914","volume":"57","author":"Doak","year":"2024","journal-title":"J. Appl. Cryst."},{"key":"zf5029_bb16","doi-asserted-by":"crossref","first-page":"4511","DOI":"10.1038\/s41467-020-18156-7","volume":"11","author":"Echelmeier","year":"2020","journal-title":"Nat. Commun."},{"key":"zf5029_bb17","doi-asserted-by":"crossref","first-page":"641","DOI":"10.1038\/nphoton.2010.176","volume":"4","author":"Emma","year":"2010","journal-title":"Nat. Photon."},{"key":"zf5029_bb18","doi-asserted-by":"crossref","first-page":"486","DOI":"10.1107\/S0907444910007493","volume":"66","author":"Emsley","year":"2010","journal-title":"Acta Cryst. D"},{"key":"zf5029_bb19","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1038\/nmeth.4195","volume":"14","author":"Fuller","year":"2017","journal-title":"Nat. Methods"},{"key":"zf5029_bb20","doi-asserted-by":"crossref","first-page":"102662","DOI":"10.1016\/j.sbi.2023.102662","volume":"82","author":"Garman","year":"2023","journal-title":"Curr. Opin. Struct. Biol."},{"key":"zf5029_bb21","doi-asserted-by":"crossref","first-page":"749","DOI":"10.1107\/S2052252524005591","volume":"11","author":"Gotthard","year":"2024","journal-title":"IUCrJ"},{"key":"zf5029_bb22","doi-asserted-by":"crossref","first-page":"051302","DOI":"10.1063\/5.0167164","volume":"17","author":"Gu","year":"2023","journal-title":"Biomicrofluidics"},{"key":"zf5029_bb901","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1038\/nmeth.2887","volume":"11","author":"Hattne","year":"2014","journal-title":"Nat. Methods"},{"key":"zf5029_bb23","first-page":"20251217692331","author":"Hinchliffe","year":"2025","journal-title":"bioRxiv"},{"key":"zf5029_bb24","unstructured":"Horrell, S., Axford, D., Devenish, N. E., Ebrahim, A., Hough, M. A., Sherrell, D. A., Storm, S. L. S., Tews, I., Worrall, J. A. R. & Owen, R. L. (2021). JoVE 62200."},{"key":"zf5029_bb25","first-page":"1128\/cmr00036","volume":"10","author":"Jacoby","year":"2009","journal-title":"Clin. Microbiol. Rev. 22. doi:"},{"key":"zf5029_bb26","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/bs.mie.2024.10.002","volume":"709","author":"Jaho","year":"2024","journal-title":"Methods Enzymol."},{"key":"zf5029_bb27","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/bs.mie.2024.10.008","volume":"709","author":"Kamps","year":"2024","journal-title":"Methods Enzymol."},{"key":"zf5029_bb28","first-page":"20250925673893","author":"Kang","year":"2025","journal-title":"bioRxiv"},{"key":"zf5029_bb29","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1038\/s41467-020-14434-6","volume":"11","author":"Kno\u0161ka","year":"2020","journal-title":"Nat. Commun."},{"key":"zf5029_bb30","doi-asserted-by":"crossref","first-page":"1086","DOI":"10.1107\/S160057751701030X","volume":"24","author":"Kubo","year":"2017","journal-title":"J. Synchrotron Rad."},{"key":"zf5029_bb31","doi-asserted-by":"crossref","first-page":"044003","DOI":"10.1063\/1.4972069","volume":"4","author":"Kupitz","year":"2017","journal-title":"Struct. Dyn."},{"key":"zf5029_bb32","doi-asserted-by":"crossref","first-page":"e02073","DOI":"10.1128\/AAC.02073-20","volume":"65","author":"Lang","year":"2021","journal-title":"Antimicrob. Agents Chemother."},{"key":"zf5029_bb33","doi-asserted-by":"crossref","first-page":"899","DOI":"10.3390\/biom10060899","volume":"10","author":"Lang","year":"2020","journal-title":"Biomolecules"},{"key":"zf5029_bb34","doi-asserted-by":"crossref","first-page":"13349","DOI":"10.1021\/acscatal.2c03754","volume":"12","author":"Lu\u010di\u0107","year":"2022","journal-title":"ACS Catal."},{"key":"zf5029_bb35","doi-asserted-by":"crossref","first-page":"658","DOI":"10.1107\/S0021889807021206","volume":"40","author":"McCoy","year":"2007","journal-title":"J. Appl. Cryst."},{"key":"zf5029_bb36","doi-asserted-by":"crossref","first-page":"979","DOI":"10.1038\/s41592-019-0553-1","volume":"16","author":"Mehrabi","year":"2019","journal-title":"Nat. Methods"},{"key":"zf5029_bb37","doi-asserted-by":"crossref","first-page":"1167","DOI":"10.1126\/science.aaw9904","volume":"365","author":"Mehrabi","year":"2019","journal-title":"Science"},{"key":"zf5029_bb38","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1107\/S2052252519016865","volume":"7","author":"Monteiro","year":"2020","journal-title":"IUCrJ"},{"key":"zf5029_bb39","doi-asserted-by":"crossref","first-page":"610","DOI":"10.1107\/S2052252522006418","volume":"9","author":"Moreno-Chicano","year":"2022","journal-title":"IUCrJ"},{"key":"zf5029_bb40","doi-asserted-by":"crossref","first-page":"1074","DOI":"10.1107\/S2052252519011655","volume":"6","author":"Moreno-Chicano","year":"2019","journal-title":"IUCrJ"},{"key":"zf5029_bb41","doi-asserted-by":"crossref","first-page":"1074","DOI":"10.1107\/S0907444909029436","volume":"65","author":"Moriarty","year":"2009","journal-title":"Acta Cryst. D"},{"key":"zf5029_bb42","doi-asserted-by":"crossref","first-page":"054302","DOI":"10.1063\/1.4928706","volume":"2","author":"Mueller","year":"2015","journal-title":"Struct. Dyn."},{"key":"zf5029_bb43","doi-asserted-by":"crossref","first-page":"1081","DOI":"10.1107\/S1600576721005720","volume":"54","author":"Nam","year":"2021","journal-title":"J. Appl. Cryst."},{"key":"zf5029_bb44","first-page":"20250713663488","author":"Narayanasamy","year":"2025","journal-title":"bioRxiv"},{"key":"zf5029_bb45","doi-asserted-by":"crossref","first-page":"109648","DOI":"10.1016\/j.celrep.2021.109648","volume":"36","author":"Nasrallah","year":"2021","journal-title":"Cell. Rep."},{"key":"zf5029_bb46","doi-asserted-by":"crossref","first-page":"752","DOI":"10.1038\/35021099","volume":"406","author":"Neutze","year":"2000","journal-title":"Nature"},{"key":"zf5029_bb47","doi-asserted-by":"crossref","first-page":"25120","DOI":"10.1021\/jacs.3c04991","volume":"145","author":"Nguyen","year":"2023","journal-title":"J. Am. Chem. Soc."},{"key":"zf5029_bb48","doi-asserted-by":"crossref","unstructured":"Olmos, J. L., Pandey, S., Martin-Garcia, J. M., Calvey, G., Katz, A., Knoska, J., Kupitz, C., Hunter, M. S., Liang, M., Oberthuer, D., Yefanov, O., Wiedorn, M., Heyman, M., Holl, M., Pande, K., Barty, A., Miller, M. D., Stern, S., Roy-Chowdhury, S., Coe, J., Nagaratnam, N., Zook, J., Verburgt, J., Norwood, T., Poudyal, I., Xu, D., Koglin, J., Seaberg, M. H., Zhao, Y., Bajt, S., Grant, T., Mariani, V., Nelson, G., Subramanian, G., Bae, E., Fromme, R., Fung, R., Schwander, P., Frank, M., White, T. A., Weierstall, U., Zatsepin, N., Spence, J., Fromme, P., Chapman, H. N., Pollack, L., Tremblay, L., Ourmazd, A., Phillips, G. N. & Schmidt, M. (2018). BMC Biol. 16, 59.","DOI":"10.1186\/s12915-018-0524-5"},{"key":"zf5029_bb49","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1107\/S2059798317002996","volume":"73","author":"Owen","year":"2017","journal-title":"Acta Cryst. D"},{"key":"zf5029_bb50","doi-asserted-by":"crossref","first-page":"668","DOI":"10.1107\/S2059798323005429","volume":"79","author":"Owen","year":"2023","journal-title":"Acta Cryst. D"},{"key":"zf5029_bb903","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1042\/bj2950295","volume":"295","author":"Page","year":"1993","journal-title":"Biochem. J."},{"key":"zf5029_bb51","doi-asserted-by":"crossref","first-page":"878","DOI":"10.1107\/S2052252521008125","volume":"8","author":"Pandey","year":"2021","journal-title":"IUCrJ"},{"key":"zf5029_bb52","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1016\/j.sbi.2020.06.019","volume":"65","author":"Pearson","year":"2020","journal-title":"Curr. Opin. Struct. Biol."},{"key":"zf5029_bb53","doi-asserted-by":"crossref","first-page":"576","DOI":"10.1111\/febs.15880","volume":"289","author":"Poddar","year":"2022","journal-title":"FEBS J."},{"key":"zf5029_bb54","doi-asserted-by":"crossref","first-page":"901","DOI":"10.1107\/S2052252520010374","volume":"7","author":"Rabe","year":"2020","journal-title":"IUCrJ"},{"key":"zf5029_bb55","doi-asserted-by":"crossref","first-page":"839","DOI":"10.1007\/s43630-024-00558-x","volume":"23","author":"Sandelin","year":"2024","journal-title":"Photochem. Photobiol. Sci."},{"key":"zf5029_bb56","doi-asserted-by":"crossref","first-page":"116","DOI":"10.3390\/cryst10020116","volume":"10","author":"Schmidt","year":"2020","journal-title":"Crystals"},{"key":"zf5029_bb57","doi-asserted-by":"crossref","first-page":"901","DOI":"10.1038\/s41592-018-0180-2","volume":"15","author":"Schulz","year":"2018","journal-title":"Nat. Methods"},{"key":"zf5029_bb58","doi-asserted-by":"crossref","first-page":"6553","DOI":"10.1038\/s41467-025-61631-2","volume":"16","author":"Schulz","year":"2025","journal-title":"Nat. Commun."},{"key":"zf5029_bb59","doi-asserted-by":"crossref","first-page":"1372","DOI":"10.1107\/S1600577515016938","volume":"22","author":"Sherrell","year":"2015","journal-title":"J. Synchrotron Rad."},{"key":"zf5029_bb60","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1038\/nmeth.3667","volume":"13","author":"Sierra","year":"2016","journal-title":"Nat. Methods"},{"key":"zf5029_bb61","doi-asserted-by":"crossref","first-page":"582","DOI":"10.1107\/S2052252525006645","volume":"12","author":"Smyth","year":"2025","journal-title":"IUCrJ"},{"key":"zf5029_bb62","doi-asserted-by":"crossref","first-page":"3472","DOI":"10.1016\/j.jmb.2019.04.002","volume":"431","author":"Tooke","year":"2019","journal-title":"J. Mol. Biol."},{"key":"zf5029_bb63","doi-asserted-by":"crossref","first-page":"1038","DOI":"10.1107\/S1600576723004405","volume":"56","author":"Vakili","year":"2023","journal-title":"J. Appl. Cryst."},{"key":"zf5029_bb64","first-page":"20250812669835","author":"von Stetten","year":"2025","journal-title":"bioRxiv"},{"key":"zf5029_bb65","doi-asserted-by":"crossref","first-page":"3309","DOI":"10.1038\/ncomms4309","volume":"5","author":"Weierstall","year":"2014","journal-title":"Nat. Commun."},{"key":"zf5029_bb66","doi-asserted-by":"crossref","first-page":"035108","DOI":"10.1063\/1.3693040","volume":"83","author":"Weierstall","year":"2012","journal-title":"Rev. Sci. Instrum."},{"key":"zf5029_bb67","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1107\/S2059798317017235","volume":"74","author":"Winter","year":"2018","journal-title":"Acta Cryst. D"},{"key":"zf5029_bb905","doi-asserted-by":"crossref","first-page":"4200","DOI":"10.21105\/joss.04200","volume":"7","author":"Wojdyr","year":"2022","journal-title":"J. Open Source Softw."},{"key":"zf5029_bb68","doi-asserted-by":"crossref","first-page":"110866","DOI":"10.1016\/j.mtcomm.2024.110866","volume":"41","author":"Zhang","year":"2024","journal-title":"Mater. Today Commun."},{"key":"zf5029_bb69","doi-asserted-by":"crossref","first-page":"778","DOI":"10.1107\/S2052252522010193","volume":"9","author":"Zielinski","year":"2022","journal-title":"IUCrJ"}],"container-title":["IUCrJ"],"original-title":[],"link":[{"URL":"https:\/\/journals.iucr.org\/m\/issues\/2026\/04\/00\/zf5029\/zf5029.pdf","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.iucr.org\/m\/issues\/2026\/04\/00\/zf5029\/zf5029.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T13:52:52Z","timestamp":1785851572000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.iucr.org\/paper?S2052252526003489"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,5]]},"references-count":73,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2026,7]]}},"alternative-id":["S2052252526003489"],"URL":"https:\/\/doi.org\/10.1107\/s2052252526003489","relation":{"is-supplemented-by":[{"id-type":"doi","id":"10.1107\/S2052252526003489\/zf5029sup1.pdf","asserted-by":"subject"},{"id-type":"doi","id":"10.2210\/pdb9toq\/pdb","asserted-by":"subject"},{"id-type":"doi","id":"10.2210\/pdb9tos\/pdb","asserted-by":"subject"},{"id-type":"doi","id":"10.2210\/pdb9tor\/pdb","asserted-by":"subject"},{"id-type":"doi","id":"10.2210\/pdb9tot\/pdb","asserted-by":"subject"},{"id-type":"doi","id":"10.2210\/pdb9to1\/pdb","asserted-by":"subject"},{"id-type":"doi","id":"10.2210\/pdb9to5\/pdb","asserted-by":"subject"},{"id-type":"doi","id":"10.2210\/pdb9top\/pdb","asserted-by":"subject"},{"id-type":"doi","id":"10.2210\/pdb9too\/pdb","asserted-by":"subject"},{"id-type":"doi","id":"10.2210\/pdb9tok\/pdb","asserted-by":"subject"},{"id-type":"doi","id":"10.2210\/pdb9tol\/pdb","asserted-by":"subject"},{"id-type":"doi","id":"10.2210\/pdb9tom\/pdb","asserted-by":"subject"},{"id-type":"doi","id":"10.2210\/pdb9ton\/pdb","asserted-by":"subject"},{"id-type":"doi","id":"10.2210\/pdb9tou\/pdb","asserted-by":"subject"},{"id-type":"doi","id":"10.2210\/pdb9tov\/pdb","asserted-by":"subject"},{"id-type":"doi","id":"10.2210\/pdb9tow\/pdb","asserted-by":"subject"}]},"ISSN":["2052-2525"],"issn-type":[{"value":"2052-2525","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,5]]},"assertion":[{"value":"IUCrJ","order":0,"name":"journal","label":"Publication","explanation":{"URL":"https:\/\/www.iucrj.org\/"},"group":{"name":"general","label":"General"}},{"value":"research papers","order":1,"name":"content_type","label":"Content type","explanation":{"URL":"https:\/\/journals.iucr.org\/m\/services\/notesforauthors.html"},"group":{"name":"general","label":"General"}},{"value":"Available","URL":"https:\/\/scripts.iucr.org\/cgi-bin\/citedin?zf5029","order":2,"name":"article_metrics","label":"Article metrics","group":{"name":"general","label":"General"}},{"value":"Yes","order":0,"name":"peer_reviewed","label":"Peer reviewed","group":{"name":"peer_review","label":"Peer review"}},{"value":"Single blind","order":1,"name":"review_process","label":"Review process","group":{"name":"peer_review","label":"Peer review"}},{"value":"8 January 2026","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication history"}},{"value":"2 April 2026","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication history"}},{"value":"5 June 2026","order":2,"name":"published_online","label":"Published online","group":{"name":"publication_history","label":"Publication history"}},{"value":"This article has supporting information","name":"supplementary_materials","label":"Supplementary materials","explanation":{"URL":"https:\/\/scripts.iucr.org\/cgi-bin\/sendsup?zf5029"}},{"value":"\u00a9 2026 Jos J. A. G. Kamps\n                      et al.","order":0,"name":"copyright","label":"Copyright","explanation":{"URL":"https:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},"group":{"name":"copyright_and_licencing","label":"Copyright and licencing"}},{"value":"Creative Commons Attribution (CC-BY)","order":1,"name":"license","label":"Licence","explanation":{"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/legalcode"},"group":{"name":"copyright_and_licencing","label":"Copyright and licencing"}}]}}