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While some reactions can be initiated with light, either naturally or using photocaged compounds, a more generally applicable approach is to mix microcrystals with reagents at varying time points prior to exposure to the X-ray beam. A powerful approach has been to combine droplet-on-demand `tape drive' sample delivery with X-ray emission spectroscopy (XES) that correlates atomic structure with the electronic states of metal ions within the sample. To the best of our knowledge, such a combined methodology has not been deployed previously on a synchrotron beamline but has been restricted to X-ray free-electron lasers. Here we describe two independent prototype experiments along the development pathway to a combined droplet-on-demand diffraction and XES system on the microfocus synchrotron beamline VMXi at Diamond Light Source. We demonstrate the collection of a high-quality serial diffraction data set from microcrystals within droplets having a volume of hundreds of picolitres deposited on a moving tape. In separate experiments on VMXi, we collected XES data from microcrystals of a copper enzyme delivered using a high-viscosity extruder. Together, these results demonstrate the feasibility of combined droplet-on-demand serial crystallography and XES experiments using a third-generation synchrotron beamline.<\/jats:p>","DOI":"10.1107\/s2052252526005658","type":"journal-article","created":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T15:12:18Z","timestamp":1783523538000},"page":"635-649","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":0,"title":["Droplet-on-demand tape drive and X-ray emission spectroscopy prototypes for time-resolved serial crystallography on VMXi at Diamond Light Source"],"prefix":"10.1107","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1793-7030","authenticated-orcid":true,"given":"Pierre","family":"Aller","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03gq8fr08","id-type":"ROR","asserted-by":"publisher"}],"department":["Research Complex at Harwell"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Sanchez-Weatherby","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03gq8fr08","id-type":"ROR","asserted-by":"publisher"}],"department":["Research Complex at Harwell"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abbey","family":"Telfer","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05etxs293","id-type":"ROR","asserted-by":"publisher"}]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Bosman","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05etxs293","id-type":"ROR","asserted-by":"publisher"}]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas","family":"Devenish","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05etxs293","id-type":"ROR","asserted-by":"publisher"}]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip","family":"Hinchliffe","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0524sp257","id-type":"ROR","asserted-by":"publisher"}],"department":["School of Biochemistry and Cellular and Molecular Medicine, Biomedical Sciences Building"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sam","family":"Horrell","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03gq8fr08","id-type":"ROR","asserted-by":"publisher"}],"department":["Research Complex at Harwell"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jophy","family":"Ip","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05etxs293","id-type":"ROR","asserted-by":"publisher"}]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard","family":"Littlewood","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05etxs293","id-type":"ROR","asserted-by":"publisher"}]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew","family":"Male","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05etxs293","id-type":"ROR","asserted-by":"publisher"}]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva","family":"Gimenez Navarro","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05etxs293","id-type":"ROR","asserted-by":"publisher"}]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Urszula","family":"Neuman","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05etxs293","id-type":"ROR","asserted-by":"publisher"}]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos J. 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