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Extension to higher scattering angles through wide-angle X-ray scattering (WAXS) extends the resolution of solution measurements to single \u00e5ngstr\u00f6ms; however, interpretation of WAXS profiles is challenging. Here we establish a practical framework for interpreting WAXS measurements in terms of repeated real-space distances and specific structural motifs in nucleic acids. We compare experimentally measured scattering profiles from two 25-base-pair DNA duplexes, an AT-rich construct and a mixed-sequence duplex. Their SAXS profiles are similar but they exhibit strikingly different WAXS signatures. By systematically varying the reciprocal-space cutoff prior to indirect Fourier transforms, we show how increasing information content sharpens real-space resolution and reveals periodic features associated with base stacking and duplex geometry. Real-space distance distributions identify the dominant atomic contributors to these features and directly connect them to backbone separations, groove widths, and long-range stacking periodicities that vary in a sequence-dependent manner. Together, these results establish WAXS as a powerful tool for probing nucleic acid architecture and, in conjunction with time-resolved measurements, dynamics, and demonstrate that WAXS measurements provide a robust and generalizable route to extracting high-resolution structural information from solution measurements.<\/jats:p>","DOI":"10.1107\/s2052252526007700","type":"journal-article","created":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T19:47:39Z","timestamp":1787341659000},"page":"650-658","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":0,"title":["Interpreting wide-angle X-ray scattering data using DNA duplexes as model systems"],"prefix":"10.1107","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1611-0127","authenticated-orcid":true,"given":"Qingyue","family":"Hu","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05bnh6r87","id-type":"ROR","asserted-by":"publisher"}],"department":["School of Applied and Engineering Physics"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vera","family":"Amirbekian","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05bnh6r87","id-type":"ROR","asserted-by":"publisher"}],"department":["School of Applied and Engineering Physics"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3027-7882","authenticated-orcid":true,"given":"Sarah","family":"Uttormark","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05bnh6r87","id-type":"ROR","asserted-by":"publisher"}],"department":["School of Applied and Engineering Physics"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hugh Robert","family":"Higinbotham","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05bnh6r87","id-type":"ROR","asserted-by":"publisher"}],"department":["School of Applied and Engineering Physics"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lois","family":"Pollack","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05bnh6r87","id-type":"ROR","asserted-by":"publisher"}],"department":["School of Applied and Engineering Physics"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"329","published-online":{"date-parts":[[2026,9,1]]},"reference":[{"key":"ro5049_bb1","doi-asserted-by":"crossref","first-page":"821","DOI":"10.1038\/325821a0","volume":"325","author":"Alexeev","year":"1987","journal-title":"Nature"},{"key":"ro5049_bb2","first-page":"20230602543451","author":"Chamberlain","year":"2023","journal-title":"bioRxiv"},{"key":"ro5049_bb3","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1016\/j.bpj.2014.06.006","volume":"107","author":"Chen","year":"2014","journal-title":"Biophys. 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