{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T22:41:12Z","timestamp":1793313672750,"version":"4.5.0"},"reference-count":22,"publisher":"International Union of Crystallography (IUCr)","issue":"2","license":[{"start":{"date-parts":[[2014,1,21]],"date-time":"2014-01-21T00:00:00Z","timestamp":1390262400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},{"start":{"date-parts":[[2014,1,21]],"date-time":"2014-01-21T00:00:00Z","timestamp":1390262400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.iucr.org\/services\/copyrightpolicy.html#TDM"}],"content-domain":{"domain":["iucr.org","wiley.com","iucrj.org"],"crossmark-restriction":true},"short-container-title":["Acta Crystallogr F Struct Biol Commun","Acta Crystallogr F","Acta Crystallogr F Struct Biol Cryst Commun","Acta Cryst F","Acta Cryst F Struct Biol Commun","Acta Cryst Sect F","Acta Cryst Sect F Struct Biol Commun","Acta Crystallogr Sect F","Acta Crystallogr Sect F Struct Biol Commun","Acta Crystallogr Sect F Struct Biol Cryst Commun"],"accepted":{"date-parts":[[2013,12,21]]},"published-print":{"date-parts":[[2014,2,1]]},"abstract":"<jats:p>The 1.90\u2005\u00c5 resolution X-ray crystal structure of glycerol dehydrogenase derived from contaminating bacteria present during routine <jats:italic>Escherichia coli<\/jats:italic> protein expression is presented. This off-target enzyme showed intrinsic affinity for Ni<jats:sup>2+<\/jats:sup>-Sepharose, migrated at the expected molecular mass for the target protein during gel filtration and was crystallized before it was realised that contamination had occurred. In this study, it is shown that liquid chromatography coupled to tandem mass spectrometry (LC-MS\/MS) can efficiently identify the protein composition of crystals in a crystallization experiment as part of a structure-determination pipeline for an unknown protein. The high-resolution X-ray data enabled sequencing directly from the electron-density maps, allowing the source of contamination to be placed within the <jats:italic>Serratia<\/jats:italic> genus. Incorporating additional protein-identity checks, such as tandem LC-MS\/MS, earlier in the protein expression, purification and crystallization workflow may have prevented the unintentional structure determination of this metabolic enzyme, which represents the first enterobacterial glycerol dehydrogenase reported to date.<\/jats:p>","DOI":"10.1107\/s2053230x13034444","type":"journal-article","created":{"date-parts":[[2014,1,20]],"date-time":"2014-01-20T16:49:10Z","timestamp":1390236550000},"page":"166-172","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":9,"title":["Structure of glycerol dehydrogenase from <i>Serratia<\/i>"],"prefix":"10.1107","volume":"70","author":[{"given":"Paul","family":"Musille","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Ortlund","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"329","published-online":{"date-parts":[[2014,1,21]]},"reference":[{"key":"gx5221_bb1","doi-asserted-by":"crossref","first-page":"1304","DOI":"10.1016\/j.bbagen.2006.03.027","volume":"1760","author":"Bolanos-Garcia","year":"2006","journal-title":"Biochim. 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