{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,1,14]],"date-time":"2025-01-14T05:11:18Z","timestamp":1736831478863,"version":"3.33.0"},"reference-count":17,"publisher":"International Union of Crystallography (IUCr)","issue":"2","license":[{"start":{"date-parts":[[2014,1,21]],"date-time":"2014-01-21T00:00:00Z","timestamp":1390262400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},{"start":{"date-parts":[[2014,1,21]],"date-time":"2014-01-21T00:00:00Z","timestamp":1390262400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.iucr.org\/services\/copyrightpolicy.html#TDM"}],"content-domain":{"domain":["iucr.org","wiley.com","iucrj.org"],"crossmark-restriction":true},"short-container-title":["Acta Crystallogr F Struct Biol Commun","Acta Crystallogr F","Acta Crystallogr F Struct Biol Cryst Commun","Acta Cryst F","Acta Cryst F Struct Biol Commun","Acta Cryst Sect F","Acta Cryst Sect F Struct Biol Commun","Acta Crystallogr Sect F","Acta Crystallogr Sect F Struct Biol Commun","Acta Crystallogr Sect F Struct Biol Cryst Commun"],"accepted":{"date-parts":[[2013,12,23]]},"published-print":{"date-parts":[[2014,2,1]]},"abstract":"<jats:p>\n                  <jats:italic>Staphylococcus aureus<\/jats:italic> is a prevalent microorganism that is capable of causing a wide range of infections and diseases. Several strains of this bacterial species have developed antibiotic resistance to methicillin and vancomycin, and higher death rates are still being reported each year owing to multidrug-resistant strains. Certain GCN5-related <jats:italic>N<\/jats:italic>-acetyltransferases (GNATs) exhibit a broad substrate range, including aminoglycosides, histones, other proteins and serotonin, and have been implicated in antibiotic drug resistance. Here, the expression, purification, crystallization and preliminary X-ray diffraction analysis of a GNAT from <jats:italic>S. aureus<\/jats:italic> (SaNAT) are reported. SaNAT was recombinantly expressed and crystallized by the hanging-drop vapour-diffusion method at 296\u2005K, and the crystals diffracted to 1.7\u2005\u00c5 resolution on the MX2 beamline at the Australian Synchrotron. The crystals belonged to space group <jats:italic>P<\/jats:italic>4<jats:sub>3<\/jats:sub>2<jats:sub>1<\/jats:sub>2, with unit-cell parameters <jats:italic>a<\/jats:italic> = <jats:italic>b<\/jats:italic> = 84.86, <jats:italic>c<\/jats:italic> = 49.06\u2005\u00c5, \u03b1 = \u03b2 = \u03b3 = 90\u00b0. A single molecule is likely to be present in the asymmetric unit. A full structural and functional analysis is currently being undertaken to provide novel insights into the protein function, which in turn may provide a basis for drug design.<\/jats:p>","DOI":"10.1107\/s2053230x13034493","type":"journal-article","created":{"date-parts":[[2014,1,20]],"date-time":"2014-01-20T16:49:10Z","timestamp":1390236550000},"page":"211-214","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":0,"title":["Purification, crystallization and preliminary X-ray diffraction analysis of the <i>N<\/i>-acetyltransferase SAV0826 from <i>Staphylococcus aureus<\/i>"],"prefix":"10.1107","volume":"70","author":[{"given":"Parul","family":"Srivastava","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yogesh B.","family":"Khandokar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jade K.","family":"Forwood","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"329","published-online":{"date-parts":[[2014,1,21]]},"reference":[{"key":"nj5177_bb1","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1107\/S0907444910048675","volume":"67","author":"Battye","year":"2011","journal-title":"Acta Cryst. 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Biol."},{"key":"nj5177_bb12","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1110\/ps.041163505","volume":"14","author":"Sandy","year":"2005","journal-title":"Protein Sci."},{"key":"nj5177_bb13","unstructured":"Shittu, A. O. & Lin, J. (2006). BMC Infect. Dis. 6, 125."},{"key":"nj5177_bb14","doi-asserted-by":"crossref","first-page":"S19","DOI":"10.1016\/j.ijid.2010.05.009","volume":"14","author":"Stefani","year":"2010","journal-title":"Int. J. Infect. Dis."},{"key":"nj5177_bb15","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/j.pep.2005.01.016","volume":"41","author":"Studier","year":"2005","journal-title":"Protein Expr. Purif."},{"key":"nj5177_bb16","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1007\/s10096-001-0643-7","volume":"21","author":"Trautmann","year":"2002","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"nj5177_bb17","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1016\/j.abb.2004.09.003","volume":"433","author":"Vetting","year":"2005","journal-title":"Arch. Biochem. Biophys."}],"container-title":["Acta Crystallographica Section F Structural Biology Communications"],"original-title":[],"link":[{"URL":"https:\/\/journals.iucr.org\/f\/issues\/2014\/02\/00\/nj5177\/nj5177.pdf","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.iucr.org\/f\/issues\/2014\/02\/00\/nj5177\/nj5177.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,13]],"date-time":"2025-01-13T09:31:21Z","timestamp":1736760681000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.iucr.org\/paper?S2053230X13034493"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,1,21]]},"references-count":17,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2014,2]]}},"alternative-id":["S2053230X13034493"],"URL":"https:\/\/doi.org\/10.1107\/s2053230x13034493","relation":{},"ISSN":["2053-230X"],"issn-type":[{"type":"electronic","value":"2053-230X"}],"subject":[],"published":{"date-parts":[[2014,1,21]]},"assertion":[{"value":"Acta Crystallographica Section F: Structural Biology Communications","order":0,"name":"journal","label":"Publication","explanation":{"URL":"https:\/\/journals.iucr.org\/f"},"group":{"name":"general","label":"General"}},{"value":"crystallization communications","order":1,"name":"content_type","label":"Content type","explanation":{"URL":"https:\/\/journals.iucr.org\/f\/services\/notesforauthors.html"},"group":{"name":"general","label":"General"}},{"value":"Available","URL":"https:\/\/scripts.iucr.org\/cgi-bin\/citedin?nj5177","order":2,"name":"article_metrics","label":"Article metrics","group":{"name":"general","label":"General"}},{"value":"Yes","order":0,"name":"peer_reviewed","label":"Peer reviewed","group":{"name":"peer_review","label":"Peer review"}},{"value":"Single blind","order":1,"name":"review_process","label":"Review process","group":{"name":"peer_review","label":"Peer review"}},{"value":"4 October 2013","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication history"}},{"value":"23 December 2013","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication history"}},{"value":"21 January 2014","order":2,"name":"published_online","label":"Published online","group":{"name":"publication_history","label":"Publication history"}},{"value":"\u00a9 2014 International Union of Crystallography","order":0,"name":"copyright","label":"Copyright","explanation":{"URL":"https:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},"group":{"name":"copyright_and_licensing","label":"Copyright and licencing"}}]}}