{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T13:21:21Z","timestamp":1770038481772,"version":"3.49.0"},"reference-count":27,"publisher":"International Union of Crystallography (IUCr)","issue":"2","license":[{"start":{"date-parts":[[2014,1,28]],"date-time":"2014-01-28T00:00:00Z","timestamp":1390867200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},{"start":{"date-parts":[[2014,1,28]],"date-time":"2014-01-28T00:00:00Z","timestamp":1390867200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.iucr.org\/services\/copyrightpolicy.html#TDM"}],"content-domain":{"domain":["iucr.org","wiley.com","iucrj.org"],"crossmark-restriction":true},"short-container-title":["Acta Crystallogr F Struct Biol Commun","Acta Crystallogr F","Acta Crystallogr F Struct Biol Cryst Commun","Acta Cryst F","Acta Cryst F Struct Biol Commun","Acta Cryst Sect F","Acta Cryst Sect F Struct Biol Commun","Acta Crystallogr Sect F","Acta Crystallogr Sect F Struct Biol Commun","Acta Crystallogr Sect F Struct Biol Cryst Commun"],"accepted":{"date-parts":[[2013,12,30]]},"published-print":{"date-parts":[[2014,2,1]]},"abstract":"<jats:p>Endoglucanases are important enzymes that are involved in the modification and degradation of cellulose. Filamentous fungi such as <jats:italic>Aspergillus terreus<\/jats:italic> are effective biomass degraders in nature owing to their capacity to produce an enzymatic arsenal of glycoside hydrolases, including endoglucanase from glycoside hydrolase family 12 (GH12). The <jats:italic>A. terreus<\/jats:italic> GH12 endoglucanase was cloned and overexpressed in <jats:italic>A. nidulans<\/jats:italic>, purified and crystallized. A single crystal was obtained from a solution consisting of 2\u2005<jats:italic>M<\/jats:italic> ammonium sulfate, 5%(<jats:italic>v<\/jats:italic>\/<jats:italic>v<\/jats:italic>) 2-propanol. X-ray diffraction data were collected to a resolution of 1.85\u2005\u00c5 using synchrotron radiation and a preliminary molecular-replacement solution was obtained in the trigonal space group <jats:italic>P<\/jats:italic>3<jats:sub>2<\/jats:sub>21. The unit-cell parameters were <jats:italic>a<\/jats:italic> = <jats:italic>b<\/jats:italic> = 103.24, <jats:italic>c<\/jats:italic> = 48.96\u2005\u00c5.<\/jats:p>","DOI":"10.1107\/s2053230x13034936","type":"journal-article","created":{"date-parts":[[2014,1,27]],"date-time":"2014-01-27T19:50:52Z","timestamp":1390852252000},"page":"267-270","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":9,"title":["Expression, purification, crystallization and preliminary X-ray diffraction analysis of <i>Aspergillus terreus<\/i> endo-\u03b2-1,4-glucanase from glycoside hydrolase family 12"],"prefix":"10.1107","volume":"70","author":[{"given":"Fernando","family":"Segato","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriela L.","family":"Berto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Evandro","family":"Ares de Ara\u00fajo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jo\u00e3o Renato","family":"Muniz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9496-4174","authenticated-orcid":true,"given":"Igor","family":"Polikarpov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"329","published-online":{"date-parts":[[2014,1,28]]},"reference":[{"key":"dp5058_bb1","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1107\/S0907444909052925","volume":"66","author":"Adams","year":"2010","journal-title":"Acta Cryst. D"},{"key":"dp5058_bb2","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1093\/bioinformatics\/16.2.135","volume":"16","author":"Beitz","year":"2000","journal-title":"Bioinformatics"},{"key":"dp5058_bb3","first-page":"3939","volume":"7","author":"Chidi","year":"2008","journal-title":"Afr. J. Biotechnol."},{"key":"dp5058_bb4","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1107\/S0907444905036693","volume":"62","author":"Evans","year":"2006","journal-title":"Acta Cryst. D"},{"key":"dp5058_bb5","unstructured":"Gasteiger, E., Hoogland, C., Gattiker, A., Duvaud, S., Wilkins, M. R., Appel, R. D. & Bairoch, A. (2005). The Proteomics Protocols Handbook, edited by J. M. Walker, pp. 571-607. Totowa: Humana Press."},{"key":"dp5058_bb30","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1107\/S0909049508034870","volume":"16","author":"Guimar\u00e3es","year":"2009","journal-title":"J. Synchrotron Rad."},{"key":"dp5058_bb6","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1007\/s00294-010-0308-0","volume":"56","author":"Han","year":"2010","journal-title":"Curr. Genet."},{"key":"dp5058_bb7","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1042\/bj2800309","volume":"280","author":"Henrissat","year":"1991","journal-title":"J. Biochem."},{"key":"dp5058_bb8","doi-asserted-by":"crossref","first-page":"804","DOI":"10.1126\/science.1137016","volume":"315","author":"Himmel","year":"2007","journal-title":"Science"},{"key":"dp5058_bb9","first-page":"196264","volume":"2012","author":"Jahromi","year":"2012","journal-title":"J. Biomed. Biotechnol."},{"key":"dp5058_bb10","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1107\/S0907444909047337","volume":"66","author":"Kabsch","year":"2010","journal-title":"Acta Cryst. D"},{"key":"dp5058_bb11","doi-asserted-by":"crossref","first-page":"660","DOI":"10.1107\/S0907444902003360","volume":"58","author":"Khademi","year":"2002","journal-title":"Acta Cryst. D"},{"key":"dp5058_bb12","doi-asserted-by":"crossref","first-page":"506","DOI":"10.1128\/MMBR.66.3.506-577.2002","volume":"66","author":"Lynd","year":"2002","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"dp5058_bb13","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1016\/0022-2836(68)90205-2","volume":"33","author":"Matthews","year":"1968","journal-title":"J. Mol. Biol."},{"key":"dp5058_bb14","doi-asserted-by":"crossref","first-page":"658","DOI":"10.1107\/S0021889807021206","volume":"40","author":"McCoy","year":"2007","journal-title":"J. Appl. Cryst."},{"key":"dp5058_bb15","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1021\/ac60147a030","volume":"31","author":"Miller","year":"1959","journal-title":"Anal. Chem."},{"key":"dp5058_bb16","doi-asserted-by":"crossref","first-page":"785","DOI":"10.1038\/nmeth.1701","volume":"8","author":"Petersen","year":"2011","journal-title":"Nature Methods"},{"key":"dp5058_bb17","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1016\/j.funbio.2012.01.004","volume":"116","author":"Picart","year":"2012","journal-title":"Fungal Biol."},{"key":"dp5058_bb18","doi-asserted-by":"crossref","first-page":"734","DOI":"10.1107\/S0907444997007233","volume":"53","author":"Polikarpov","year":"1997","journal-title":"Acta Cryst. D"},{"key":"dp5058_bb19","doi-asserted-by":"crossref","first-page":"e59069","DOI":"10.1371\/journal.pone.0059069","volume":"8","author":"Prates","year":"2013","journal-title":"PLoS One"},{"key":"dp5058_bb20","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1006\/jmbi.2001.4583","volume":"308","author":"Sandgren","year":"2001","journal-title":"J. Mol. Biol."},{"key":"dp5058_bb21","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1016\/j.enzmictec.2012.04.008","volume":"51","author":"Segato","year":"2012","journal-title":"Enzyme Microb. Technol."},{"key":"dp5058_bb22","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1186\/1754-6834-5-21","volume":"5","author":"Segato","year":"2012","journal-title":"Biotechnol. Biofuels"},{"key":"dp5058_bb23","doi-asserted-by":"crossref","first-page":"815","DOI":"10.1016\/0006-291X(67)90391-9","volume":"28","author":"Shapiro","year":"1967","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"dp5058_bb24","doi-asserted-by":"crossref","first-page":"5199","DOI":"10.1128\/AEM.00199-11","volume":"77","author":"Wang","year":"2011","journal-title":"Appl. Environ. Microbiol."},{"key":"dp5058_bb25","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1107\/S0907444910045749","volume":"67","author":"Winn","year":"2011","journal-title":"Acta Cryst. D"},{"key":"dp5058_bb26","doi-asserted-by":"crossref","first-page":"797","DOI":"10.1002\/bit.20282","volume":"88","author":"Zhang","year":"2004","journal-title":"Biotechnol. Bioeng."}],"container-title":["Acta Crystallographica Section F Structural Biology Communications"],"original-title":[],"link":[{"URL":"https:\/\/journals.iucr.org\/f\/issues\/2014\/02\/00\/dp5058\/dp5058.pdf","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.iucr.org\/f\/issues\/2014\/02\/00\/dp5058\/dp5058.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,13]],"date-time":"2025-01-13T09:31:08Z","timestamp":1736760668000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.iucr.org\/paper?S2053230X13034936"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,1,28]]},"references-count":27,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2014,2]]}},"alternative-id":["S2053230X13034936"],"URL":"https:\/\/doi.org\/10.1107\/s2053230x13034936","relation":{},"ISSN":["2053-230X"],"issn-type":[{"value":"2053-230X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,1,28]]},"assertion":[{"value":"Acta Crystallographica Section F: Structural Biology Communications","order":0,"name":"journal","label":"Publication","explanation":{"URL":"https:\/\/journals.iucr.org\/f"},"group":{"name":"general","label":"General"}},{"value":"crystallization communications","order":1,"name":"content_type","label":"Content type","explanation":{"URL":"https:\/\/journals.iucr.org\/f\/services\/notesforauthors.html"},"group":{"name":"general","label":"General"}},{"value":"Available","URL":"https:\/\/scripts.iucr.org\/cgi-bin\/citedin?dp5058","order":2,"name":"article_metrics","label":"Article metrics","group":{"name":"general","label":"General"}},{"value":"Yes","order":0,"name":"peer_reviewed","label":"Peer reviewed","group":{"name":"peer_review","label":"Peer review"}},{"value":"Single blind","order":1,"name":"review_process","label":"Review process","group":{"name":"peer_review","label":"Peer review"}},{"value":"13 October 2013","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication history"}},{"value":"30 December 2013","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication history"}},{"value":"28 January 2014","order":2,"name":"published_online","label":"Published online","group":{"name":"publication_history","label":"Publication history"}},{"value":"\u00a9 2014 International Union of Crystallography","order":0,"name":"copyright","label":"Copyright","explanation":{"URL":"https:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},"group":{"name":"copyright_and_licensing","label":"Copyright and licencing"}}]}}