{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,1,14]],"date-time":"2025-01-14T05:11:48Z","timestamp":1736831508642,"version":"3.33.0"},"reference-count":16,"publisher":"International Union of Crystallography (IUCr)","issue":"4","license":[{"start":{"date-parts":[[2014,3,25]],"date-time":"2014-03-25T00:00:00Z","timestamp":1395705600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},{"start":{"date-parts":[[2014,3,25]],"date-time":"2014-03-25T00:00:00Z","timestamp":1395705600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html#TDM"}],"content-domain":{"domain":["iucr.org","wiley.com","iucrj.org"],"crossmark-restriction":true},"short-container-title":["Acta Crystallogr F Struct Biol Commun","Acta Crystallogr F","Acta Crystallogr F Struct Biol Cryst Commun","Acta Cryst F","Acta Cryst F Struct Biol Commun","Acta Cryst Sect F","Acta Cryst Sect F Struct Biol Commun","Acta Crystallogr Sect F","Acta Crystallogr Sect F Struct Biol Commun","Acta Crystallogr Sect F Struct Biol Cryst Commun"],"published-print":{"date-parts":[[2014,4,1]]},"abstract":"<jats:p><jats:italic>Acinetobacter baumannii<\/jats:italic>causes bacteraemia, pneumonia, other respiratory-tract and urinary-tract infections in humans. OXA-23 carbapenemase-producing<jats:italic>A. baumannii<\/jats:italic>K0420859 (<jats:italic>A. baumannii<\/jats:italic>OXA-23) is resistant to carbapenem, a common antibacterial drug. To develop an efficient and novel antibacterial drug against<jats:italic>A. baumannii<\/jats:italic>OXA-23, D-alanine-D-alanine ligase, which is essential in bacterial cell-wall synthesis, is of interest. Here, the D-alanine-D-alanine ligase (<jats:italic>AbDdl<\/jats:italic>) gene from<jats:italic>A. baumannii<\/jats:italic>OXA-23 was cloned and expressed, and the<jats:italic>Ab<\/jats:italic>Ddl protein was purified and crystallized; this enzyme can be used as a novel target for an antibacterial drug against<jats:italic>A. baumannii<\/jats:italic>OXA-23. The<jats:italic>Ab<\/jats:italic>Ddl crystal diffracted to a resolution of 2.8\u2005\u00c5 and belonged to the orthorhombic space group<jats:italic>P<\/jats:italic>2<jats:sub>1<\/jats:sub>2<jats:sub>1<\/jats:sub>2<jats:sub>1<\/jats:sub>, with unit-cell parameters<jats:italic>a<\/jats:italic>= 113.4,<jats:italic>b<\/jats:italic>= 116.7,<jats:italic>c<\/jats:italic>= 176.5\u2005\u00c5, a corresponding<jats:italic>V<\/jats:italic><jats:sub>M<\/jats:sub>of 2.8\u2005\u00c5<jats:sup>3<\/jats:sup>\u2005Da<jats:sup>\u22121<\/jats:sup>and a solvent content of 56.3%, and six protomers in the asymmetric unit.<\/jats:p>","DOI":"10.1107\/s2053230x14005081","type":"journal-article","created":{"date-parts":[[2014,3,24]],"date-time":"2014-03-24T19:52:33Z","timestamp":1395690753000},"page":"505-508","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":2,"title":["Expression, crystallization and preliminary X-ray crystallographic analysis of<scp>D<\/scp>-alanine-<scp>D<\/scp>-alanine ligase from OXA-23-producing<i>Acinetobacter baumannii<\/i>K0420859"],"prefix":"10.1107","volume":"70","author":[{"given":"Kim-Hung","family":"Huynh","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huyen-Thi","family":"Tran","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tan-Viet","family":"Pham","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ho-Phuong-Thuy","family":"Ngo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sun-Shin","family":"Cha","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyung Min","family":"Chung","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sang Hee","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lin-Woo","family":"Kang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"329","published-online":{"date-parts":[[2014,3,25]]},"reference":[{"key":"pu5420_bb1","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1107\/S0907444909052925","volume":"66","author":"Adams","year":"2010","journal-title":"Acta Cryst. 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Protein Crystallogr. 43, contribution 7."}],"container-title":["Acta Crystallographica Section F Structural Biology Communications"],"original-title":[],"link":[{"URL":"http:\/\/journals.iucr.org\/f\/issues\/2014\/04\/00\/pu5420\/pu5420.pdf","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/journals.iucr.org\/f\/issues\/2014\/04\/00\/pu5420\/pu5420.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,13]],"date-time":"2025-01-13T09:38:58Z","timestamp":1736761138000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.iucr.org\/paper?S2053230X14005081"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,3,25]]},"references-count":16,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2014,4]]}},"alternative-id":["S2053230X14005081"],"URL":"https:\/\/doi.org\/10.1107\/s2053230x14005081","relation":{},"ISSN":["2053-230X"],"issn-type":[{"type":"electronic","value":"2053-230X"}],"subject":[],"published":{"date-parts":[[2014,3,25]]},"assertion":[{"value":"Acta Crystallographica Section F: Structural Biology Communications","name":"journal","label":"Journal","explanation":{"URL":"http:\/\/journals.iucr.org\/f"}},{"value":"crystallization communications","name":"content_type","label":"Content type","explanation":{"URL":"http:\/\/journals.iucr.org\/f\/issues\/2011\/01\/00\/me0435\/index.html"}},{"value":"Yes","order":0,"name":"peer_reviewed","label":"Peer reviewed","group":{"name":"peer_review","label":"Peer review"}},{"value":"Single blind","order":1,"name":"review_process","label":"Review process","group":{"name":"peer_review","label":"Peer review"}},{"value":"24 January 2014","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication history"}},{"value":"5 March 2014","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication history"}},{"value":"25 March 2014","order":2,"name":"published_online","label":"Published online","group":{"name":"publication_history","label":"Publication history"}},{"value":"\u00a9 2014 International Union of Crystallography","order":0,"name":"copyright","label":"Copyright","explanation":{"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},"group":{"name":"copyright_and_licensing","label":"Copyright and licencing"}}]}}